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In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results.Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today.

Transcription Factors --- Sequence Analysis, DNA --- Transfection --- Transcription factors --- Facteurs de transcription --- analysis --- methods --- Laboratory manuals --- Manuels de laboratoire --- Laboratory manuals. --- analysis. --- methods. --- Sequence analysis, dna --- Methods. --- Analysis. --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Genetic transcription factors --- Proteins --- Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology

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Genes --- Genome --- Sequence Analysis, DNA --- Genomes --- Genomics --- DNA --- Gènes --- Génomes --- Génomique --- ADN --- Periodicals --- Research --- Périodiques --- Recherche --- Genes. --- Genome. --- Sequence Analysis, DNA. --- Chemistry --- Engineering --- Health Sciences --- Life Sciences --- Biochemistry --- Biotechnology --- Genetics --- Immunology --- Biology --- General and Others --- genomic structure --- genomic function --- genomes --- gene analysis --- bioinformatic analysis --- genomic data --- Genomes. --- Genomics. --- Research. --- Deoxyribonucleic acid --- Desoxyribonucleic acid --- Thymonucleic acid --- TNA (Nucleic acid) --- Deoxyribose --- Nucleic acids --- Units of heredity --- Units of inheritance --- Heredity --- Molecular genetics --- Proteome --- Analysis, DNA Sequence --- DNA Sequence Determination --- DNA Sequence Determinations --- DNA Sequencing --- Determination, DNA Sequence --- Determinations, DNA Sequence --- Sequence Determinations, DNA --- DNA Sequence Analysis --- Sequence Determination, DNA --- Analyses, DNA Sequence --- DNA Sequence Analyses --- Sequence Analyses, DNA --- Sequencing, DNA --- Molecular Sequence Annotation --- Cistron --- Gene --- Genetic Materials --- Cistrons --- Genetic Material --- Material, Genetic --- Materials, Genetic --- Genome research --- Haploidy --- Sequence Analysis, DNA/ --- Gènes. --- Génomes. --- Gènes. --- Génomes. --- Génomique --- Gènes --- Génomes

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Retroelements are ancient mobile DNA found in most organisms. Long dismissed as useless, selfish or "junk" DNA, they were thought to be mere intracellular parasites from our distant evolutionary past. Together with their mutant relatives, L1 sequences constitute almost 50% of the mammalian genome. L1s can retrotranspose in a defined window of the neuronal differentiation, changing the genetic information in single neurons in a "random" fashion, allowing the brain to develop in distinct different ways. Such strategy contributes to expand the number of functionally distinct neurons that could be produced from a given stem cell gene pool. This characteristic of variety and flexibility may contribute to the uniqueness of an individual brain, even between genetically identical twins. These mobile elements may be part of conserved core process responsible for evoking facilitated complex non-random phenotypical variation on which selection may act. A detailed understanding of the basic mechanisms of L1 activity may shed light on one possible mechanism for generating neural diversity. The book results from a fascinating and stimulating exchange of ideas at the interface of the complexity of brain organization and function, the mechanisms for generating diversity and genetic mobility. This meeting of leading geneticists, molecular biologists and neuroscientists was organized by the Fondation IPSEN. Its ambitious goal was to expand the current limits of research in neurobiology not only to the benefit of those interested in the cellular and molecular processes but also for the understanding of high-level cognitive functions and the understanding of complex mental diseases. The reader can judge how far the book achieves this.

genetica --- Neuropathology --- Human genetics --- neurologie --- medische genetica --- Retroelements --- Sequence Analysis, DNA. --- Biodiversity. --- Brain --- Genetic Variation. --- Neurogenetics. --- Neurogénétique --- genetics. --- physiology. --- Medicine. --- Human genetics. --- Neurosciences. --- Neurology. --- Medicine & Public Health. --- Human Genetics. --- Nervous system --- Genetics --- Neurosciences --- Genetic aspects --- Heredity, Human --- Human biology --- Physical anthropology --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Medicine --- Neuropsychiatry --- Diseases --- Neurology .

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Advances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Molecular biology --- Analytical biochemistry --- Polymerase Chain Reaction --- Sequence Analysis, DNA --- Polymerase chain reaction --- Réaction en chaîne de la polymérase --- methods --- laboratory manuals --- Laboratory manuals --- Manuels de laboratoire --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals. --- Réaction en chaîne de la polymérase --- Polymerization --- Polymerisation --- Polymers --- Polymers and polymerization --- Synthesis of polymers --- Chemical reactions --- Synthesis --- laboratory manuals. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cell biology.

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DNA --- Molecular genetics --- Mobile genetic elements --- ADN --- Génétique moléculaire --- Eléments génétiques mobiles --- Periodicals --- Périodiques --- Genomics --- Genomics. --- Sequence Analysis, DNA. --- Mobile genetic elements. --- Genetic elements, Mobile --- Genetic mobile elements --- Mobile DNA --- Mobile DNA sequences --- Mobile elements, Genetic --- Genome research --- Genomes --- Analysis, DNA Sequence --- DNA Sequence Determination --- DNA Sequence Determinations --- DNA Sequencing --- Determination, DNA Sequence --- Determinations, DNA Sequence --- Sequence Determinations, DNA --- DNA Sequence Analysis --- Sequence Determination, DNA --- Analyses, DNA Sequence --- DNA Sequence Analyses --- Sequence Analyses, DNA --- Sequencing, DNA --- Research --- DNA rearrangements --- DNA recombination --- Molecular Sequence Annotation --- Human Genome Project --- Genome --- DNA. --- Comparative Genomics --- Comparative Genomic --- Genomic, Comparative --- Genomics, Comparative --- Genetics --- dna rearrangements --- dna recombination