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Molecular biology --- General microbiology --- Escherichia coli --- Salmonella typhimurium --- Escherichia Coli --- Salmonella Typhimurium --- Escherichia coli. --- Salmonella typhimurium. --- #abib:almm --- Bacillus typhimurium --- Salmonella --- E. coli (Bacterium) --- Escherichia --- Salmonella typhimurium LT2 --- Diffusely Adherent E. coli --- Enteroaggregative E. coli --- Enteroinvasive E. coli --- Enteroinvasive Escherichia coli --- Alkalescens-Dispar Group --- Diffusely Adherent Escherichia coli --- E coli --- EAggEC --- Enteroaggregative Escherichia coli --- Escherichia coli Proteins --- Escherichia Coli. --- Salmonella Typhimurium. --- Biology --- Microbiology & Immunology --- Health & Biological Sciences --- Salmonella tyhimurmium --- Escherichian coli --- Monograph --- Bacillus coli --- Bacterium coli --- Bacterium coli commune --- Enterococcus coli
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Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
Plasmids --- Escherichia coli --- Genetic vectors --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- E. coli (Bacterium) --- Escherichia --- Paragenes --- Cytoplasmic inheritance --- Mobile genetic elements --- Extrachromosomal DNA --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition
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Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.
Escherichia coli --- Gene expression --- Genetics --- E. coli (Bacterium) --- Escherichia --- Genes --- Genetic regulation --- Expression --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Escherichia coli - Genetics
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Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. Coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. Coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. Coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.
Escherichia coli. --- Systems biology. --- Escherichia coli --- Systems biology --- Systems Biology --- Escherichia --- Computational Biology --- Biology --- Enterobacteriaceae --- Biological Science Disciplines --- Gram-Negative Facultatively Anaerobic Rods --- Gammaproteobacteria --- Natural Science Disciplines --- Proteobacteria --- Gram-Negative Bacteria --- Bacteria --- Disciplines and Occupations --- Organisms --- Biochemistry --- Biology - General --- Microbiology & Immunology --- Chemistry --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Biochemistry. --- Biotechnology. --- Life sciences. --- Microbial genetics. --- Microorganisms --- Biosciences --- Sciences, Life --- Biological chemistry --- Chemical composition of organisms --- Physiological chemistry --- E. coli (Bacterium) --- Genetics --- Composition --- Microbiology. --- Microbial genomics. --- Life Sciences. --- Biochemistry, general. --- Microbial Genetics and Genomics. --- Applied Microbiology. --- Microbiology --- Science --- Chemical engineering --- Genetic engineering --- Medical sciences --- Genomics --- Microbial genetics --- Microbial biology
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Escherichia coli --- Pathogenic bacteria --- Vegetation monitoring --- Runoff --- Manures --- Vegetation monitoring. --- Measurement --- Health aspects --- Data processing --- Monitoring, Vegetation --- Plants --- Environmental monitoring --- Dung --- Dungs --- Fertilizers and manures --- Manure --- Animal waste --- Crop residues --- Fertilizers --- Organic fertilizers --- Flowoff --- Melt runoff --- Meltwater runoff --- Rainfall runoff --- Run-off --- Snow cover runoff --- Snow dump runoff --- Snow melt runoff --- Snowmelt-induced runoff --- Snowmelt runoff --- Snowmelt water runoff --- Storm runoff --- Storm water runoff --- Stormwater runoff --- Water runoff, Snowmelt --- Water runoff, Storm --- Hydrologic cycle --- Bacteria, Pathogenic --- Disease germs --- Bacteria --- Pathogenic microorganisms --- Bacterial diseases --- Medical bacteriology --- E. coli (Bacterium) --- Escherichia --- Monitoring
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Enterobacteriaceae --- Watershed ecology --- Escherichia coli --- Salmonella --- Enterobacteriaceae. --- Escherichia coli. --- Salmonella. --- Watershed ecology. --- Middle Atlantic States. --- United States. --- Ecology --- Salmonellae --- Salmonellas --- E. coli (Bacterium) --- Escherichia --- Coliform bacilli --- Coliform bacteria --- Coliforms --- Colon bacilli --- Enteric bacilli --- Enteric bacteria --- Enterics (Bacteria) --- Enterobacteria --- Fecal coliform bacteria --- Fecal coliforms --- Gram-negative bacteria --- ABŞ --- ABSh --- Ameerika Ühendriigid --- America (Republic) --- Amerika Birlăshmish Shtatlary --- Amerika Birlăşmi Ştatları --- Amerika Birlăşmiş Ştatları --- Amerika ka Kelenyalen Jamanaw --- Amerika Qūrama Shtattary --- Amerika Qŭshma Shtatlari --- Amerika Qushma Shtattary --- Amerika (Republic) --- Amerikai Egyesült Államok --- Amerikanʹ Veĭtʹsėndi͡avks Shtattnė --- Amerikări Pĕrleshu̇llĕ Shtatsem --- Amerikas Forenede Stater --- Amerikayi Miatsʻyal Nahangner --- Ameriketako Estatu Batuak --- Amirika Carékat --- AQSh --- Ar. ha-B. --- Arhab --- Artsot ha-Berit --- Artzois Ha'bris --- Bí-kok --- Ē.P.A. --- É.-U. --- EE.UU. --- Egyesült Államok --- ĒPA --- Estados Unidos --- Estados Unidos da América do Norte --- Estados Unidos de América --- Estaos Xuníos --- Estaos Xuníos d'América --- Estatos Unitos --- Estatos Unitos d'America --- Estats Units d'Amèrica --- Ètats-Unis d'Amèrica --- États-Unis d'Amérique --- ÉU --- Fareyniḳṭe Shṭaṭn --- Feriene Steaten --- Feriene Steaten fan Amearika --- Forente stater --- FS --- Hēnomenai Politeiai Amerikēs --- Hēnōmenes Politeies tēs Amerikēs --- Hiwsisayin Amerikayi Miatsʻeal Tērutʻiwnkʻ --- Istadus Unidus --- Jungtinės Amerikos valstybės --- Mei guo --- Mei-kuo --- Meiguo --- Mî-koet --- Miatsʻyal Nahangner --- Miguk --- Na Stàitean Aonaichte --- NSA --- S.U.A. --- SAD --- Saharat ʻAmērikā --- SASht --- Severo-Amerikanskie Shtaty --- Severo-Amerikanskie Soedinennye Shtaty --- Si͡evero-Amerikanskīe Soedinennye Shtaty --- Sjedinjene Američke Države --- Soedinennye Shtaty Ameriki --- Soedinennye Shtaty Severnoĭ Ameriki --- Soedinennye Shtaty Si͡evernoĭ Ameriki --- Spojené obce severoamerické --- Spojené staty americké --- SShA --- Stadoù-Unanet Amerika --- Stáit Aontaithe Mheiriceá --- Stany Zjednoczone --- Stati Uniti --- Stati Uniti d'America --- Stâts Unîts --- Stâts Unîts di Americhe --- Steatyn Unnaneysit --- Steatyn Unnaneysit America --- SUA --- Sŭedineni amerikanski shtati --- Sŭedinenite shtati --- Tetã peteĩ reko Amérikagua --- U.S. --- U.S.A. --- United States of America --- Unol Daleithiau --- Unol Daleithiau America --- Unuiĝintaj Ŝtatoj de Ameriko --- US --- USA --- Usono --- Vaeinigte Staatn --- Vaeinigte Staatn vo Amerika --- Vereinigte Staaten --- Vereinigte Staaten von Amerika --- Verenigde State van Amerika --- Verenigde Staten --- VS --- VSA --- Wááshindoon Bikéyah Ałhidadiidzooígíí --- Wilāyāt al-Muttaḥidah --- Wilāyāt al-Muttaḥidah al-Amirīkīyah --- Wilāyāt al-Muttaḥidah al-Amrīkīyah --- Yhdysvallat --- Yunaeted Stet --- Yunaeted Stet blong Amerika --- ZDA --- Združene države Amerike --- Zʹi͡ednani Derz͡havy Ameryky --- Zjadnośone staty Ameriki --- Zluchanyi͡a Shtaty Ameryki --- Zlucheni Derz͡havy --- ZSA --- Mid-Atlantic States --- Middle Atlantic Region --- Middle Colonies --- Middle States --- United States
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