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The application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Microbial enzymes --- Microbial biotechnology --- Biotransformation (Metabolism) --- Biotechnology --- Biotransformation (Metabolism) -- Laboratory manuals. --- Microbial biotechnology -- Laboratory manuals. --- Microbial enzymes -- Biotechnology -- Laboratory manuals. --- Biotransformation --- Activation, Metabolic --- Metabolic activation --- Microorganisms --- Industrial microbiology --- Biotechnological microorganisms --- Bioactivation (Metabolism) --- Biodegradation (Metabolism) --- Metabolism --- Pharmacology --- Microbiology. --- Microbial biology --- Biology

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The development of recombinant DNA techniques over the last 20 years has greatly expanded the opportunities for using microorganisms to produce a broad range of valuable substances. In Microbial Processes and Products, outstanding leaders in using microorganisms as cell factories describe in detail their best laboratory procedures for many processes and products mediated by microorganisms. An overview chapter describes how to develop strain improvement programs and strategies to optimize fermentation processes. Taking advantage of the most recent developments in such processes, the authors offer step-by-step experimental methods for the optimal design of microbial metabolite production, including semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination, L-lysine, vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids b-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation. Additionally, the use of phenylacetyl-CoA catabolon for enzymatic synthesis of penicillins, aromatic biotransformations, synthesis of new bioplastics, biosensor design, or synthesis of drug vehicles, and the development of a phosphate encoding gene as a reporter and to monitor gene expression are illustrated. The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection. Additional chapters offer techniques for analysis of antimicrobial metabolites and carotenoids, volatile sulfur compounds, metabolic pathway fluxes, gene expression arrays, proteome analysis, bacterial modulation of the innate immune response, bioleaching activity, and heavy metal remediation. Finally, three overview chapters on transport of biological material, deposit of biological material for patent purposes, and protection of biotechnological inventions are shown. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Enzymes and Biotransformations, describes in detail cutting-edge techniques for the screening, evolution, production, immobilization, and use of enzymes. Wide-ranging and practical, Microbial Processes and Products offers laboratory and industrial scientists a wealth of readily reproducible techniques for the successful microbial generation of biochemical products to serve the needs of human health, nutrition, and environmental protection.

biotechnologie --- recombinant DNA --- micro-organismen --- Biotechnology --- Microbial biotechnology --- Industrial Microbiology --- Methodology --- methods --- Biotechnology. --- Electronic books. -- local. --- Microbial biotechnology -- Methodology. --- Microbiology --- Biology --- Technology --- Biological Science Disciplines --- Technology, Industry, and Agriculture --- Natural Science Disciplines --- Technology, Industry, Agriculture --- Disciplines and Occupations --- Bioengineering --- Mechanical Engineering --- Engineering & Applied Sciences --- Methodology. --- Microorganisms --- Industrial microbiology --- Biotechnological microorganisms --- Chemical engineering --- Genetic engineering --- Microbiology. --- Microbial biology

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The application of microbiological methods to the extraction of metals from minerals has definitely gained a prominent role and is supported by the several bioleaching and biooxidation processes operating in different sites over the world. In recent years new techniques and new results about proteomic and bioinformatics are bringing a new perspective on the microbial processes. This book focuses on the basic aspects of the process with special emphasis on recent contributions regarding the chemical and microbial aspects of the bioleaching process and the use of microorganisms in the treatment of complex ores and concentrates. It has been written by recognized researchers in order to introduce the main subjects of microbial processing to undergraduate and postgraduate students, scientists working in this field as well as interested industrialists.

Sulfides --- Minerals --- Microbial biotechnology. --- Metallurgy. --- Biotechnology. --- Microorganisms --- Biotechnology --- Industrial microbiology --- Biotechnological microorganisms --- Biomining --- Mineral bioprocessing --- Mineral biotechnology --- Metallurgy --- Mining engineering --- Materials. --- Microbiology. --- Biochemical engineering. --- Mineral resources. --- Bioinformatics. --- Microbial ecology. --- Metallic Materials. --- Applied Microbiology. --- Biochemical Engineering. --- Mineral Resources. --- Microbial Ecology. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Deposits, Mineral --- Mineral deposits --- Mineral resources --- Mines and mining --- Mining --- Natural resources --- Geology, Economic --- Bio-process engineering --- Bioprocess engineering --- Biochemistry --- Chemical engineering --- Environmental microbiology --- Ecology --- Microbiology --- Microbial biology --- Engineering --- Engineering materials --- Industrial materials --- Engineering design --- Manufacturing processes --- Data processing --- Materials --- Metals. --- Metallic elements --- Chemical elements --- Ores --- Sulfures (minéralogie) --- Minéraux --- Microorganismes --- Biotechnologie

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Plant biotechnology --- Botanical chemistry --- Microbial biotechnology --- Biochemistry --- Genetic Engineering --- Genetics, Microbial --- Molecular Biology --- Plants --- Recombinant Proteins --- genetics --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Plant --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Microorganisms --- Crop biotechnology --- Crops --- Biotechnology --- Bioreactors --- Botany --- Plant Proteins --- Ethnopharmacology --- Genetic Phenomena --- Virus Physiological Phenomena --- Bacterial Physiological Phenomena --- Genome, Microbial --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Industrial microbiology --- Biotechnological microorganisms --- Agricultural biotechnology --- Chemistry --- biotechnologie --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant --- Biochemistry. --- Genetic Engineering. --- Genetics, Microbial. --- Molecular Biology. --- Plants. --- Recombinant Proteins. --- genetics.

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Microbial biotechnology --- Lignocellulose --- Ethanol as fuel --- Biomass energy --- Renewable energy sources --- Agriculture and energy --- Agriculture and energy. --- Biomass energy. --- Ethanol as fuel. --- Microbial biotechnology. --- Renewable energy sources. --- Industrial applications --- United States. --- Alternate energy sources --- Alternative energy sources --- Energy sources, Renewable --- Sustainable energy sources --- Power resources --- Renewable natural resources --- Microorganisms --- Biotechnology --- Industrial microbiology --- Biotechnological microorganisms --- Ligno-cellulose --- Lignocellulosics --- Cellulose --- Lignin --- Ethanol fuel --- Fuel ethanol --- Fuel --- Bio-energy (Biomass energy) --- Bioenergy (Biomass energy) --- Biofuels --- Biological fuels --- Energy, Biomass --- Microbial energy conversion --- Energy conversion --- Microbial fuel cells --- Refuse as fuel --- Waste products as fuel --- Energy and agriculture --- Energy in agriculture --- Biomass as fuel --- Renewable fuels --- ABŞ --- ABSh --- Ameerika Ühendriigid --- America (Republic) --- Amerika Birlăshmish Shtatlary --- Amerika Birlăşmi Ştatları --- Amerika Birlăşmiş Ştatları --- Amerika ka Kelenyalen Jamanaw --- Amerika Qūrama Shtattary --- Amerika Qŭshma Shtatlari --- Amerika Qushma Shtattary --- Amerika (Republic) --- Amerikai Egyesült Államok --- Amerikanʹ Veĭtʹsėndi͡avks Shtattnė --- Amerikări Pĕrleshu̇llĕ Shtatsem --- Amerikas Forenede Stater --- Amerikayi Miatsʻyal Nahangner --- Ameriketako Estatu Batuak --- Amirika Carékat --- AQSh --- Ar. ha-B. --- Arhab --- Artsot ha-Berit --- Artzois Ha'bris --- Bí-kok --- Ē.P.A. --- É.-U. --- EE.UU. --- Egyesült Államok --- ĒPA --- Estados Unidos --- Estados Unidos da América do Norte --- Estados Unidos de América --- Estaos Xuníos --- Estaos Xuníos d'América --- Estatos Unitos --- Estatos Unitos d'America --- Estats Units d'Amèrica --- Ètats-Unis d'Amèrica --- États-Unis d'Amérique --- ÉU --- Fareyniḳṭe Shṭaṭn --- Feriene Steaten --- Feriene Steaten fan Amearika --- Forente stater --- FS --- Hēnomenai Politeiai Amerikēs --- Hēnōmenes Politeies tēs Amerikēs --- Hiwsisayin Amerikayi Miatsʻeal Tērutʻiwnkʻ --- Istadus Unidus --- Jungtinės Amerikos valstybės --- Mei guo --- Mei-kuo --- Meiguo --- Mî-koet --- Miatsʻyal Nahangner --- Miguk --- Na Stàitean Aonaichte --- NSA --- S.U.A. --- SAD --- Saharat ʻAmērikā --- SASht --- Severo-Amerikanskie Shtaty --- Severo-Amerikanskie Soedinennye Shtaty --- Si͡evero-Amerikanskīe Soedinennye Shtaty --- Sjedinjene Američke Države --- Soedinennye Shtaty Ameriki --- Soedinennye Shtaty Severnoĭ Ameriki --- Soedinennye Shtaty Si͡evernoĭ Ameriki --- Spojené obce severoamerické --- Spojené staty americké --- SShA --- Stadoù-Unanet Amerika --- Stáit Aontaithe Mheiriceá --- Stany Zjednoczone --- Stati Uniti --- Stati Uniti d'America --- Stâts Unîts --- Stâts Unîts di Americhe --- Steatyn Unnaneysit --- Steatyn Unnaneysit America --- SUA --- Sŭedineni amerikanski shtati --- Sŭedinenite shtati --- Tetã peteĩ reko Amérikagua --- U.S. --- U.S.A. --- United States of America --- Unol Daleithiau --- Unol Daleithiau America --- Unuiĝintaj Ŝtatoj de Ameriko --- US --- USA --- Usono --- Vaeinigte Staatn --- Vaeinigte Staatn vo Amerika --- Vereinigte Staaten --- Vereinigte Staaten von Amerika --- Verenigde State van Amerika --- Verenigde Staten --- VS --- VSA --- Wááshindoon Bikéyah Ałhidadiidzooígíí --- Wilāyāt al-Muttaḥidah --- Wilāyāt al-Muttaḥidah al-Amirīkīyah --- Wilāyāt al-Muttaḥidah al-Amrīkīyah --- Yhdysvallat --- Yunaeted Stet --- Yunaeted Stet blong Amerika --- ZDA --- Združene države Amerike --- Zʹi͡ednani Derz͡havy Ameryky --- Zjadnośone staty Ameriki --- Zluchanyi͡a Shtaty Ameryki --- Zlucheni Derz͡havy --- ZSA