Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Analytical biochemistry --- Proteins --- Protéines --- analysis --- handbooks --- Analysis --- Handbooks, manuals, etc --- Analyse --- Guides, manuels, etc. --- -#WDIR:vfm2 --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Mass spectrometry. --- Proteins -- Analysis -- Handbooks, manuals, etc. --- Proteins -- Analysis. --- Proteins. --- Proteomics. --- Handbooks --- Clinical Laboratory Techniques --- Peptides --- Amino Acids, Peptides, and Proteins --- Investigative Techniques --- Publication Formats --- Chemicals and Drugs --- Publication Characteristics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- handbooks. --- Handbooks. --- Protéines --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- #WDIR:vfm2 --- Proteins - Analysis - Handbooks, manuals, etc. --- Proteins - analysis - handbooks

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

From Reviews of the First Edition "...a must for researchers in protein chemistry and especially those with less experience..." -Doody's Health Sciences Book Review Journal "...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field."-Bioseparation "...a very useful tool for daily work." -Nahrung-Food Since publication of the bestselling first edition of John Walker's widely acclaimed Protein Protocols Handbook in 1996, there have been many highly significant developments in protein biochemistry. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.

Analytical biochemistry --- Proteins --- Peptides --- Protéines --- analysis --- Analysis --- Laboratory manuals --- Laboratory Manuals --- Analyse --- Manuels de laboratoire --- Biochemistry. --- Life sciences. --- Proteins -- Analysis -- Laboratory manuals. --- Proteomics. --- Science. --- Amino Acids, Peptides, and Proteins --- Molecular Conformation --- Investigative Techniques --- Molecular Structure --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Biochemical Phenomena --- Chemical Phenomena --- Phenomena and Processes --- Protein Conformation --- Chemistry Techniques, Analytical --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Protéines --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- Immunology. --- Immunobiology --- Life sciences --- Serology --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- PROTEINS --- ANALYSIS --- LABORATORY MANUALS

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!

Proteins --- Peptides --- Analysis --- Methodology. --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Methodology --- Moleculaire biologie --- Moleculaire biologie. --- Life sciences. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

577.112 --- Molecular biology --- -Proteins --- -#WSCH:WBIO --- 577.112 Proteins --- Proteins --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Research --- -Methodology --- Methodology. --- #WSCH:WBIO --- Research&delete& --- Methodology --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and robust results.

Biochemistry. --- Post-translational modification . --- Protein Science. --- Posttranslational Modification. --- Co-translational modification --- Cotranslational modification --- Modification, Post-translational --- Post-translation protein modification --- Post-translational protein modification --- Posttranslation protein modification --- Posttranslational modification --- Posttranslational protein modification --- Genetic translation --- Proteins --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Synthesis --- Composition --- Post-translational modification.