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Cell Imaging Techniques : Methods and Protocols
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ISBN: 1627030565 1627030557 Year: 2013 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters  covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls  Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

Cell imaging techniques : methods and protocols
Authors: ---
ISBN: 9781588291578 158829157X 9781592599936 1592599931 128083191X 9786610831913 Year: 2006 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Keywords

Cells --- Microscopy --- Cytological Techniques --- Cytology --- Molecular biology --- Imaging systems in biology --- Cytologie --- Microscopie --- Biologie moléculaire --- Imagerie en biologie --- Laboratory Manuals --- methods --- Cytology. --- Imaging systems in biology. --- Microscopy. --- Molecular biology. --- Electronic books. -- local. --- Clinical Laboratory Techniques --- Anatomy --- Investigative Techniques --- Publication Formats --- Phenomena and Processes --- Diagnostic Imaging --- Optics and Photonics --- Diagnostic Techniques and Procedures --- Physics --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Publication Characteristics --- Natural Science Disciplines --- Diagnosis --- Disciplines and Occupations --- Cell Physiological Phenomena --- Methods --- Biology --- Health & Biological Sciences --- Biological imaging systems --- Molecular biochemistry --- Molecular biophysics --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Cell biology --- Cellular biology --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Cytologists --- Optics --- Human anatomy. --- Biochemistry. --- Anatomy. --- Cell Biology. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Anatomy, Human --- Human biology --- Human body --- Composition

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