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A manual that details the techniques of electrofusion and electroporation by researchers who were the first to show that the platelet membrane glycoproteins GP IIb and GP IIIa are associated in a complex which triggered interest in electrofusion.

Hybridomas. --- Cell hybridization.

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Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods

Cancer --- Immunohistochemistry. --- In situ hybridization. --- Fluorescence in situ hybridization. --- Immunodiagnosis. --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization --- Nucleic acid hybridization --- Immunohistology --- Histochemistry --- Immunochemistry --- Diagnosis --- Immunological aspects

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Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer.This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step

Pathology, Molecular. --- Prostate --- Rectum --- Immunochemistry. --- In situ hybridization. --- Cancer. --- Diseases. --- Nucleic acid hybridization --- Biochemistry --- Immunity --- Proctology --- Molecular pathology --- Molecular biology --- Physiology, Pathological

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In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical

Immunohistochemistry. --- Medicine. --- Pathology. --- Immunohistochemistry --- Pathology, Molecular --- Immunochemistry --- Genetic Techniques --- Histocytochemistry --- Nucleic Acid Hybridization --- Immunologic Tests --- Staining and Labeling --- Histocytological Preparation Techniques --- Immunologic Techniques --- Biochemistry --- Histology --- Laboratory Techniques and Procedures --- Histological Techniques --- Investigative Techniques --- Cytological Techniques --- Clinical Laboratory Techniques --- Diagnosis --- Chemistry --- Biological Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anatomy --- Natural Science Disciplines --- Disciplines and Occupations --- In Situ Hybridization --- Gene Expression Profiling --- Biology --- Medicine --- Health & Biological Sciences --- Pathology --- Microbiology & Immunology --- Technique --- Pathology, Molecular. --- In situ hybridization. --- Nucleic acid hybridization --- Molecular pathology --- Molecular biology --- Physiology, Pathological --- Technique.

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This book is an extended argument for abandoning the species rank. Instead, the author proposes that the rank of "species" be replaced by a pluralistic and multi-level view. In such a view, all clades including the smallest identifiable one would be named and studied within a phylogenetic context. What are currently called "species" represent different sorts of things depending on the sort of organisms and processes being considered. This is already the case, but is not formally recognized by those scientists using the species rank in their work. Adopting a rankless taxonomy at all levels would enhance academic studies of evolution and ecology and yield practical benefits in areas of public concern such as conservation. KEY FEATURES Proposes the replacement of restrictive species concepts with a pluralistic view Suggests abandoning the formal taxonomic rank of "species" Considers zoological, botanical, and microbiological aspects of the species level Deals with practical issues such as conservation, inventories, and field guides.

Species. --- NATURE / Animals. --- Speciation (Biology) --- Biology --- Genetics --- Hybridization --- Organisms --- Animals. --- Animal kingdom --- Beasts --- Fauna --- Native animals --- Native fauna --- Wild animals --- Wildlife --- Human-animal relationships --- Zoology