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The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented byimmunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the

Molecular genetics. --- Lungs --- Breast --- Immunohistochemistry. --- In situ hybridization. --- Cancer. --- Nucleic acid hybridization --- Immunohistology --- Histochemistry --- Immunochemistry --- Lung cancer --- Genetics --- Molecular biology

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Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods

Cancer --- Immunohistochemistry. --- In situ hybridization. --- Fluorescence in situ hybridization. --- Immunodiagnosis. --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization --- Nucleic acid hybridization --- Immunohistology --- Histochemistry --- Immunochemistry --- Diagnosis --- Immunological aspects

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The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes in vitro. The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

Analytical biochemistry --- Neoplastic Processes --- Pathologic Processes --- Neoplasms --- Pathological Conditions, Signs and Symptoms --- Diseases --- Neoplasm Metastasis --- Metastases, Neoplasm --- Metastasis, Neoplasm --- Neoplasm Metastases --- Metastase --- Metastasis --- Metastases --- Symptoms and General Pathology --- Benign Neoplasms --- Malignancy --- Malignant Neoplasms --- Neoplasia --- Neoplasm --- Neoplasms, Benign --- Cancer --- Tumors --- Benign Neoplasm --- Cancers --- Malignancies --- Malignant Neoplasm --- Neoplasias --- Neoplasm, Benign --- Neoplasm, Malignant --- Neoplasms, Malignant --- Tumor --- Medical Oncology --- Pathological Processes --- Processes, Pathologic --- Processes, Pathological --- Processes, Neoplastic --- secondary --- Inositol phosphates. --- Inositol phosphates - Laboratory manuals. --- Phospholipids - Laboratory manuals. --- Phospholipids. --- Glycerophospholipids --- Phosphotransferases (Alcohol Group Acceptor) --- Investigative Techniques --- Phosphotransferases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Phosphatidic Acids --- Transferases --- Glycerophosphates --- Enzymes --- Phospholipids --- Enzymes and Coenzymes --- Membrane Lipids --- Chemicals and Drugs --- Lipids --- Phosphatidylinositol 3-Kinases --- 1-Phosphatidylinositol 4-Kinase --- Phosphatidylinositols --- Clinical Laboratory Techniques --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Clinical Laboratory Diagnoses --- Clinical Laboratory Testings --- Clinical Laboratory Tests --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Clinical Laboratory Test --- Clinical Laboratory Testing --- Diagnose, Clinical Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Laboratory Test, Clinical --- Laboratory Testing, Clinical --- Technique, Clinical Laboratory --- Test, Clinical Laboratory --- Testing, Clinical Laboratory --- Laboratories --- Medical Laboratory Science --- Clinical Laboratory Services --- Inositide Phospholipids --- Inositol Phospholipids --- Phosphatidyl Inositol --- Phosphatidylinositol --- Inositide Phospholipid --- Inositol Phosphoglyceride --- Inositol Phosphoglycerides --- Inositol Phospholipid --- Phosphoinositide --- Phosphoinositides --- PtdIns --- Inositol, Phosphatidyl --- Phosphoglyceride, Inositol --- Phosphoglycerides, Inositol --- Phospholipid, Inositide --- Phospholipid, Inositol --- Phospholipids, Inositide --- Phospholipids, Inositol --- PI 4-Kinase --- Phosphatidylinositiol Kinase --- Phosphatidylinositol Kinase Type II --- Phosphoinositide Kinase --- Phosphatidylinositol 4-Kinase --- PtdIns 4-Kinase --- 1 Phosphatidylinositol 4 Kinase --- Kinase, Phosphatidylinositiol --- Kinase, Phosphoinositide --- PI 4 Kinase --- Phosphatidylinositol 4 Kinase --- PtdIns 4 Kinase --- PI 3-Kinase --- PI-3K --- PI3 Kinases --- PI3-Kinase --- Phosphoinositide 3 Kinases --- Phosphoinositide 3-Hydroxykinase --- PtdIns 3-Kinases --- PI-3 Kinase --- Phosphatidylinositol-3-OH Kinase --- PtdIns 3-Kinase --- 3-Hydroxykinase, Phosphoinositide --- Kinase, PI-3 --- Kinase, Phosphatidylinositol-3-OH --- Kinases, PI3 --- Kinases, Phosphoinositide 3 --- PI 3 Kinase --- PI3 Kinase --- Phosphatidylinositol 3 Kinases --- Phosphatidylinositol 3 OH Kinase --- Phosphoinositide 3 Hydroxykinase --- PtdIns 3 Kinase --- PtdIns 3 Kinases --- Lipid --- Cell Membrane Lipid --- Cell Membrane Lipids --- Membrane Lipid --- Lipid, Cell Membrane --- Lipid, Membrane --- Lipids, Cell Membrane --- Lipids, Membrane --- Membrane Lipid, Cell --- Membrane Lipids, Cell --- Cell Membrane --- Membranes --- Coenzymes and Enzymes --- Phosphatides --- Phospholipid --- Biocatalysts --- Biocatalyst --- Enzyme --- Molecular Mechanisms of Pharmacological Action --- Calcium Glycerophosphate --- Glycerolphosphates --- Glycerolphosphate --- Glycerophosphate --- Glycerophosphate, Calcium --- Transferase --- Ammonium Phosphatidate --- Diacylglycerophosphates --- Phosphatidic Acid --- Acid, Phosphatidic --- Acids, Phosphatidic --- Phosphatidate, Ammonium --- Kinase --- Kinases --- Phosphotransferases, ATP --- Transphosphorylases --- ATP Phosphotransferases --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Glycerophospholipid --- Phosphoglyceride --- Phosphoglycerides --- 616-006.6 --- 616-006.6 Malignant epithelial tumours. Carcinomata. Cancer --- Malignant epithelial tumours. Carcinomata. Cancer --- Cancer metastasis --- Dissemination of cancer --- Metastatic cancer --- Neoplasm metastasis --- Spread of cancer --- Tumor dissemination --- Tumor metastasis --- Tumor spread --- Pathology --- Cancer invasiveness --- Cancer of unknown primary origin --- Research --- Methodology --- Dissemination --- 57.08 --- 57.08 Biological techniques. Experimental methods. Equipment --- Biological techniques. Experimental methods. Equipment --- Isoelectric focusing. --- Hydrogen-ion concentration. --- Hydrogen-ion activity --- pH (Chemistry) --- Acidity function --- Electrofocusing --- Focusing, Isoelectric --- Analytical chemistry --- Chemistry, Organic --- Electrophoresis --- Qualitative --- Nucleic acid hybridization. --- Nucleic acid probes. --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Cytogenetics --- Cytology --- Hybridization --- Technique --- RNA-protein interactions --- Interactions, RNA-protein --- Protein-RNA interactions --- RNA-protein binding --- Protein binding --- RNA-protein interactions. --- Nucleic Acids --- Amino Acids, Peptides, and Proteins --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemical Phenomena --- Proteins --- RNA --- Chemistry --- Physical Sciences & Mathematics --- Biochemistry --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Gene Products, Protein --- Gene Proteins --- Protein --- Protein Gene Products --- Proteins, Gene --- Chemical Phenomenon --- Chemical Process --- Physical Chemistry Phenomena --- Physical Chemistry Process --- Physicochemical Phenomenon --- Physicochemical Process --- Chemical Concepts --- Chemical Processes --- Physical Chemistry Concepts --- Physical Chemistry Processes --- Physicochemical Concepts --- Physicochemical Phenomena --- Physicochemical Processes --- Chemical Concept --- Chemistry Process, Physical --- Chemistry Processes, Physical --- Concept, Chemical --- Concept, Physical Chemistry --- Concept, Physicochemical --- Concepts, Chemical --- Concepts, Physical Chemistry --- Concepts, Physicochemical --- Phenomena, Chemical --- Phenomena, Physical Chemistry --- Phenomena, Physicochemical --- Phenomenon, Chemical --- Phenomenon, Physicochemical --- Physical Chemistry Concept --- Physicochemical Concept --- Process, Chemical --- Process, Physical Chemistry --- Process, Physicochemical --- Processes, Chemical --- Processes, Physical Chemistry --- Processes, Physicochemical --- Nucleic Acid --- Acid, Nucleic --- Acids, Nucleic --- Diagnostic Techniques and Procedures --- Analysis.