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Phytohistology. Phytocytology --- Plant cell culture --- Cell culture --- Plant cell culture. --- Plant tissue culture. --- Basic Sciences. Biotechnology -- Micropropagation --- ALLW. --- Plant tissue culture --- Tissue culture

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Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.

Plant cell culture --- Plant tissue culture --- Plant cell culture -- Laboratory manuals. --- Plant tissue culture -- Laboratory manuals. --- Life sciences. --- Cell biology. --- Plant science. --- Botany. --- Plant physiology. --- Life Sciences. --- Cell Biology. --- Plant Sciences. --- Plant Physiology. --- Botany --- Plants --- Physiology --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Tissue culture --- Cell culture --- Cytology. --- Floristic botany --- Plant cell culture - Laboratory manuals --- Plant tissue culture - Laboratory manuals --- Acqui 2006

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Applications of Plant Cell and Tissue Culture Chairman: Y. Yamada 1988 Interest in all aspects of plant cell and tissue culture is expanding rapidly as the technologies available improve and the commercial potential of these processes becomes apparent. This book covers basic plant physiology and cytology and extends to the practical exploitation of plants, both as crops per se and as sources of useful compounds produced as secondary metabolites. The reduction of plants to single cells or tissues that can be maintained in culture facilitates their manipulation by the techniques of molecular biology. Genes encoding desirable traits, for example herbicide or pathogan resistance or early flowering, can be introduced into domesticated crops by transformation of normal plant cells using a natural vector such as Agrobacterium, by the uptake of DNA into protoplasts using techniques developed for animal cells, or by direct injection of DNA into intact cells with cell walls. Application of these technologies to forest trees is especially important because the long lifespan of these species makes their improvement by conventional breeding programmes very slow. Problems of commercial exploitation considered include the storage of genetically engineered material until progeny evaluation has been completed, the stability of traits in culture, and the feasibility of adapting laboratory methods to large-scale production plants. There is also some discussion of the sociolegal aspects of genetic engineering of crop plants, and of the difficulties of marketing 'natural' compounds produced by cells under artificial conditions. Related Ciba Foundation Symposia: No 97 Better crops for food Chairman: E. A. Bell 1983 ISBN 0 272 79729 4 No 133 Plant resistance to viruses Chairman: B. D. Harris 1987 ISBN 0 471 91263 8

Plant micropropagation --- Plant Molecular Biology --- Plant Molecular Biology. --- Plant biotechnology --- Plant cell culture --- Plant tissue culture --- Congresses --- Basic Sciences. Molecular Biology --- Congresses. --- Plant propagation --- In vitro --- Plant biotechnology - Congresses --- Plant cell culture - Congresses --- Plant tissue culture - Congresses --- Plant micropropagation - Congresses --- CELLS, CULTURED --- PLANTS --- TISSUE CULTURE --- GENETICS --- GENETIC CODE --- GENETIC VECTORS --- CONGRESSES

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Histology. Cytology --- General embryology. Developmental biology --- Plant cell culture --- Plant cell development --- Plant cells and tissues --- Plant micropropagation --- Plant tissue culture --- Plants --- Plantes --- Periodicals. --- Periodicals --- Development --- Cellules et tissus --- Culture --- Périodiques --- Développement --- Micropropagation --- Cytology --- Developmental Biology --- periodicals --- Tissue Culture --- Cell Biology --- Développement --- JEX6 --- Cell Biology. --- Developmental Biology. --- Plants. --- 57 --- 576 --- 58 --- Plant --- Botany --- Plant Proteins --- Ethnopharmacology --- Biology, Developmental --- Embryonic and Fetal Development --- Growth and Development --- Cellular Biology --- Biologies, Cell --- Biologies, Cellular --- Biology, Cell --- Biology, Cellular --- Cell Biologies --- Cellular Biologies --- Biological sciences in general --- Cellular and subcellular biology. Cytology --- Agriculture Sciences --- Engineering --- Life Sciences --- Plant Pathology --- Biotechnology --- General and Others --- periodical --- in vitro culture --- USA --- 58 Botany --- 576 Cellular and subcellular biology. Cytology --- Périodiques --- EJBIOLO EJBIOMO EPUB-ALPHA-I EPUB-PER-FT JSTOR-E SPRINGER-E --- Cell development, Plant --- Flora --- Plant kingdom --- Plantae --- Vascular plants --- Vegetable kingdom --- Vegetation --- Wildlife --- In vitro plant propagation --- Micropropagation, Plant --- Plant propagation --- Plant tissues --- In vitro --- Developmental cytology --- Organisms --- Vegetative propagation --- Cells --- Plant anatomy --- Tissues --- Cell culture --- Tissue culture --- Auxology --- 58 Botanie --- Botanie