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Microorganismes et biologie moléculaire
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ISBN: 2705658319 9782705658311 Year: 1976 Publisher: Paris : Hermann,

E. coli gene expression protocols
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ISBN: 1588290085 9786610841882 1280841885 1592593011 9781588290083 Year: 2003 Volume: 205 Publisher: Totowa, NJ : Humana Press : Imprint: Humana,

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Abstract

Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.

The periplasm
Authors: ---
ISBN: 1555813984 9781555813987 9781555815806 1555815804 1555913984 1283034093 9786613034090 Year: 2007 Publisher: Washington, D.C. : ASM Press,

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Abstract

Written by an international group of eminent scientists, this new treatise is the very first in the field to provide a thorough, state-of-the-art review of the periplasm, the extracytoplasmic compartment found in gram-negative bacteria.

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