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Molecular biology --- General microbiology --- Escherichia coli --- Salmonella typhimurium --- Escherichia Coli --- Salmonella Typhimurium --- Escherichia coli. --- Salmonella typhimurium. --- #abib:almm --- Bacillus typhimurium --- Salmonella --- E. coli (Bacterium) --- Escherichia --- Salmonella typhimurium LT2 --- Diffusely Adherent E. coli --- Enteroaggregative E. coli --- Enteroinvasive E. coli --- Enteroinvasive Escherichia coli --- Alkalescens-Dispar Group --- Diffusely Adherent Escherichia coli --- E coli --- EAggEC --- Enteroaggregative Escherichia coli --- Escherichia coli Proteins --- Escherichia Coli. --- Salmonella Typhimurium. --- Biology --- Microbiology & Immunology --- Health & Biological Sciences --- Salmonella tyhimurmium --- Escherichian coli --- Monograph --- Bacillus coli --- Bacterium coli --- Bacterium coli commune --- Enterococcus coli
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Gene Expression Regulation, Bacterial. --- Escherichia coli --- 575.1 --- Diffusely Adherent E. coli --- Enteroaggregative E. coli --- Enteroinvasive E. coli --- Enteroinvasive Escherichia coli --- Alkalescens-Dispar Group --- Diffusely Adherent Escherichia coli --- E coli --- EAggEC --- Enteroaggregative Escherichia coli --- Escherichia coli Proteins --- Regulation, Gene Expression, Bacterial --- Bacterial Gene Expression Regulation --- Regulation of Gene Expression, Bacterial --- Heredity. Inheritance --- Escherichia coli. --- 575.1 Heredity. Inheritance --- Molecular biology --- Monera --- Bacillus coli --- Bacterium coli --- Bacterium coli commune --- Enterococcus coli --- Gene Expression Regulation, Bacterial
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Microbiology --- Molecular Biology --- Bacteria --- Escherichia coli --- Physiology --- -Escherichia coli --- Molecular biology --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- E. coli (Bacterium) --- Escherichia --- Germs --- Microbes --- Prokaryotes --- Bacterial physiology --- Bacteria - Physiology
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Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.
Escherichia coli --- Gene expression --- Genetics --- E. coli (Bacterium) --- Escherichia --- Genes --- Genetic regulation --- Expression --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Escherichia coli - Genetics
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Written by an international group of eminent scientists, this new treatise is the very first in the field to provide a thorough, state-of-the-art review of the periplasm, the extracytoplasmic compartment found in gram-negative bacteria.
Bacterial proteins. --- Proteins --- Escherichia coli. --- Microbiology. --- Periplasmic Proteins --- Bacterial Outer Membrane Proteins --- Escherichia coli --- Membrane Transport Proteins --- Periplasm --- Synthesis. --- physiology. --- Bacterial proteins --- Microbiology --- Synthesis --- physiology --- Microbial proteins --- Protein biosynthesis --- Protein synthesis --- Microbial biology --- Biology --- Microorganisms --- E. coli (Bacterium) --- Escherichia --- Metabolism --- Proteins - Synthesis --- Periplasmic Proteins - physiology --- Bacterial Outer Membrane Proteins - physiology --- Escherichia coli - physiology --- Membrane Transport Proteins - physiology --- Periplasm - physiology --- Acqui 2006
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