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DNA-protein interactions --- DNA-protein interactions.

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The pivotal role played by transcription factors in regulating gene expression has ensured that their study is a major area of current research activity. This book provides a comprehensive description of all the approaches and methods required to identify, isolate and characterize transcription factors. Starting with a specific DNA sequence, these protocols allow the detection and identification of proteins that bind to it and detailed analysis of the DNA binding site. This is followed by procedures for the functional assay of transcription factors and their purification. Also included are methods for the characterization of transcription factor genes, including DNA cloning. The analysis of transcription factors is of central interest to all molecular biologists involved in studying gene expression, making this book an important text for a wide range of researchers from postgraduate students to established scientists.

Transcription Factors --- DNA-protein interactions --- Genetic regulation --- Transcription factors --- Régulation génétique --- Facteurs de transcription --- physiology --- DNA-protein interactions. --- Genetic regulation. --- Transcription factors. --- physiology. --- Régulation génétique

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Conferences - Meetings --- Zinc-finger proteins --- DNA-protein interactions --- Transcription factors --- Genetic regulation --- Carcinogenesis --- Zinc fingers --- Zinc proteins --- Genetic transcription factors --- Proteins --- DNA-protein binding --- Interactions, DNA-protein --- Protein-DNA interactions --- DNA-ligand interactions --- Protein binding --- Congresses --- DNA-Binding Proteins. --- Zinc-finger proteins - Congresses. --- DNA-protein interactions - Congresses. --- Carcinogenesis - Congresses. --- Transcription factors - Congresses. --- Genetic regulation - Congresses. --- Transcription, genetic

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The study of protein-nucleic acid interactions is currently one of the most rapidly growing areas of molecular biology. DNA binding proteins are at the very heart of the regulation and control of gene expression, replication, and recombination: Enzymes that recognize and either modify or cleave specific DNA sequences are equally important to the cell. Some of the techniques reported in this volume can be used to identify previously unknown DNA binding proteins from crude cell extracts. Virtually all are capable of giving direct information on the molecular basis of the interaction—the location of the DNA binding site; the strength and specificity of binding; the identities of individual groups on specific bases involved in binding; the specific amino acid residues of the protein that interact with the DNA; or the effects of protein binding on gross conformation and local structure of DNA. The recognition of DNA sequences by proteins is a complex phenomenon, involving specific hydrogen bonding contacts to the DNA bases ("direct readout") and/or interactions with the sugar-phos­ phate backbone ("indirect readout"). The latter interactions can also be highly specific because of sequence-dependent conformational changes in the DNA. In addition, intercalation of planar aromatic amino acid side-chains between the DNA bases can occur, most notably with single-stranded DNA binding proteins. Furthermore, when bound, many DNA binding proteins induce drastic structural changes in the DNA as an integral part of their function.

DNA-protein interactions --- ADN --- DNA --- Protéine --- proteins --- Acide nucléique --- Nucleic acids --- Protéine de liaison --- Binding proteins --- Synthèse protéique --- protein synthesis --- DNA-protein interactions. --- Electronic books. -- local. --- Protein binding. --- Genetic Techniques --- DNA-Binding Proteins --- Proteins --- Investigative Techniques --- Amino Acids, Peptides, and Proteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- DNA-protein binding --- Interactions, DNA-protein --- Protein-DNA interactions --- DNA-ligand interactions --- Protein binding --- Binding, Protein --- Biochemistry --- Allosteric proteins --- Radioligand assay --- DNA. --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition