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CARBOHYDRATES --- Carbohydrate Metabolism. --- Metabolism, Carbohydrate --- Carbohydrate metabolism. --- Carbohydrates. --- Carbohydrate metabolism --- Carbohydrates --- Carbs (Carbohydrates) --- Biomolecules --- Organic compounds --- Glycomics --- Carbohydrate --- metabolism --- Metabolism. --- Carbohydrate Metabolism --- Carbohydrates - metabolism

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Hypoglycaemia --- drugs --- Carbohydrate metabolism --- therapy

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Potato UDP-Glucose : protein transglucosylase (UPTG ; EC 2.4.1.112) is an enzyme capable of autoglycosylation in vitro but its precise role in vivo is still unknown. Based on the biochemical characterization of UPTG two hypotheses have been formulated : a role in the initiation of starch biosynthesis or a role in cell wall polysaccharide biosynthesis. First the sequences of two cDNAs, called UPTG1 and UPTG2 and coding for potato UPTG were characterized in more detail and their expression was analysed in this work. A subcellular localization using antibodies against the purified potato UPTG indicates a Golgi localization of UPTG. In order to test in planta the intervention of UPTG in polysaccharide metabolism of Solanum tuberosum L., transgenic plants were produced. Some transgenic lines showed an important reduction of UPTG protein levels but they did not show any difference in their development, in the amylose/amylopectin ratio or during the cell wall signature analysis as compared to the wild type. According to the results obtained, an UPTG intervention in starch synthesis seems unlikely. Whereas indications pointing towards a role of UPTG in cell wall polysaccharide synthesis exist, the analysis of our transgenic plants did not allow to confirm this.

Solanum tuberosum --- Enzymes --- Glycosidases --- Polysaccharides --- Carbohydrate metabolism

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Carbohydrates --- Metabolism. --- Carbohydrates in human nutrition. --- Nutrition --- Carbohydrate metabolism

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plants --- Storage organs --- Starch --- Polymers --- Enzyme activity --- Carbohydrate metabolism --- Models