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Manganese nodules --- Congresses --- Nodules polymétalliques. --- Manganèse. --- Manganese nodules. --- Manganese.
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Marine Manganese Deposits
Manganese nodules --- Deep-sea manganese nodules --- Manganese ores --- Marine mineral resources --- Marine sediments --- Manganese nodules.
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Manganese nodules --- Nodules polymétalliques. --- Manganèse --- Ressources minérales sous-marines. --- Manganese nodules. --- Manganese ores. --- Marine mineral resources. --- Minerais.
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The Local Lymph Node Assay: BrdU-ELISA (LLNA:BrdU-ELISA) is a non-radioactive modification to the LLNA method for identifying potential skin sensitizing test substances and measuring the proliferation of lymphocytes they induce in the auricular lymph nodes. The method described in mouse is based on the use of measuring 5-bromo-2-deoxyuridine (BrdU) content, an analogue of thymidine, as an indicator of this proliferation. A minimum of four animals is used per dose group, with a minimum of three concentrations of the test substance, plus a concurrent negative control group and a positive control group. The experimental schedule is during 6 days. Thereafter, the animals are killed and a single cell suspension of lymph node cells (LNC) is prepared. The procedure for preparing the LNC is crucial, in particular for the small lymph nodes in NC animals. Then the BrdU content in DNA of lymphocytes is measured by ELISA using a commercial kit of by Flow Cytometry (FCM). This study includes: measurements (weighing, BrdU) and clinical daily observations. The results are expressed as the Stimulation Index (SI) obtained by calculation from the mean BrdU labelling index. The SI should be ≥1.6 for the ELISA method or ≥2.7 for the FCM method for identifying the test material as a potential skin sensitizer.
Lymph Nodes --- Lymph glands --- Lymph nodules --- Nodes, Lymph --- Nodules, Lymph --- Nodus lymphaticus --- Lymphatics --- Lymph nodes.
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The Local Lymph Node Assay: DA (LLNA: DA) is a non-radioactive modification to the LLNA method for identifying potential skin sensitizing test substances and measuring the proliferation of lymphocytes they induce in the auricular lymph nodes. The method, described in mouse (CBA/J strain), is based on measurement of the adenosine triphosphate (ATP) content by bioluminescence as an indicator of this proliferation. A minimum of four animals is used per dose group, with a minimum of three concentrations of the test substance, plus a concurrent negative control group and, if appropriate, a positive control group. The experimental schedule is during 8 days. The time from animal sacrifice to measurement of ATP should not exceed 30 min. The procedure from excision of lymph nodes to ATP measurement should be kept uniform for each animal and completed within 20 minutes. The luciferin/luciferase method is applied to measure the bioluminescence in Relative Luminescence Units (RLU). This study includes: measurements (weighing, RLU), and clinical daily observations. The results are expressed as the Stimulation Index (SI) obtained by calculation. The SI should be ¡Ý1.8 before further evaluation of the test material as a potential skin sensitizer is warranted.
Lymph nodes --- Lymph glands --- Lymph nodules --- Nodes, Lymph --- Nodules, Lymph --- Nodus lymphaticus --- Lymphatics --- Lymph nodes.
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Manganese nodules. --- Manganese mines and mining, Submarine. --- Oceanography --- Mineralogy --- Geology
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The Indian Ocean Nodule Field: Geology and Resource Potential, Second Edition, provides a view of the most recent studies along with classical theories starting from the 1960s. New concepts, hypotheses and critical appreciation of the state-of-the-art knowledge on nodule formation and resource management are featured in this new edition. The economy of the Indian Ocean has vast potential and yet it is one of the least studied oceans. Garnering economic advantage out of mineral resources from deep oceans has been a long cherished dream for the mining community. The availability of 5-metal rich polymetallic (manganese) nodules in specific areas of the Indian Ocean has lured researchers to discover more about their processes of formation, growth, distribution and enrichment. Collates, discusses and succinctly presents voluminous and scattered information from various books and journals for easy understanding and disseminationFeatures two new chapters: statistical modeling of the formation, growth, distribution and metal enrichment of nodules; and biogeochemistry of the Central Indian Ocean BasinIncludes an assessment of Indian Ocean nodules, one of the least studied oceans in comparison to samples analyzed from the world's oceans.
Geology. Earth sciences --- Geology --- Manganese nodules --- Marine mineral resources --- Indian Ocean.
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Azospirillum --- Locomotion --- genetic code --- Root nodules --- Glycoproteins --- Enzyme activity --- mutation. --- mutation --- Azospirillum brasilense --- Mastigophora
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