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La genèse des nodules de manganèse
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ISBN: 2222024633 Year: 1979 Volume: 289 Publisher: Paris : Éditions du C.N.R.S.,

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Dissertation
Contribution à l'étude génétique des nodules polymétalliques des fonds océaniques
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Year: 1991

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Dissertation
Contribution à l'étude génétique des nodules polymétalliques des fonds océaniques
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Year: 1991

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Marine manganese deposits
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ISBN: 0444415246 9786611772314 1281772313 0080870511 9780444415240 9780080870519 Year: 1977 Volume: 15 Publisher: Amsterdam ; New York : New York : Elsevier Scientific Pub. Co. ; distributors for the U.S. and Canada, Elsevier/North-Holland,

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Marine Manganese Deposits

Manganese nodules : research data and methods of investigation
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ISBN: 0306651866 Year: 1979 Publisher: New York : IFI/Plenum,

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Test No. 442B: Skin Sensitization : Local Lymph Node Assay: BrdU-ELISA or –FCM
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ISBN: 9789264090996 Year: 2018 Publisher: Paris : OECD Publishing,

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The Local Lymph Node Assay: BrdU-ELISA (LLNA:BrdU-ELISA) is a non-radioactive modification to the LLNA method for identifying potential skin sensitizing test substances and measuring the proliferation of lymphocytes they induce in the auricular lymph nodes. The method described in mouse  is based on the use of measuring 5-bromo-2-deoxyuridine (BrdU) content, an analogue of thymidine, as an indicator of this proliferation. A minimum of four animals is used per dose group, with a minimum of three concentrations of the test substance, plus a concurrent negative control group and a positive control group. The experimental schedule is during 6 days. Thereafter, the animals are killed and a single cell suspension of lymph node cells (LNC) is prepared. The procedure for preparing the LNC is crucial, in particular for the small lymph nodes in NC animals. Then the BrdU content in DNA of lymphocytes is measured by ELISA using a commercial kit of by Flow Cytometry (FCM). This study includes: measurements (weighing, BrdU) and clinical daily observations. The results are expressed as the Stimulation Index (SI) obtained by calculation from the mean BrdU labelling index. The SI should be ≥1.6 for the ELISA method or ≥2.7 for the FCM method for identifying the test material as a potential skin sensitizer.  


Book
Test No. 442A: Skin Sensitization : Local Lymph Node Assay: DA
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ISBN: 9789264090972 Year: 2010 Publisher: Paris : OECD Publishing,

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The Local Lymph Node Assay: DA (LLNA: DA) is a non-radioactive modification to the LLNA method for identifying potential skin sensitizing test substances and measuring the proliferation of lymphocytes they induce in the auricular lymph nodes. The method, described in mouse (CBA/J strain), is based on measurement of the adenosine triphosphate (ATP) content by bioluminescence as an indicator of this proliferation. A minimum of four animals is used per dose group, with a minimum of three concentrations of the test substance, plus a concurrent negative control group and, if appropriate, a positive control group. The experimental schedule is during 8 days. The time from animal sacrifice to measurement of ATP should not exceed 30 min. The procedure from excision of lymph nodes to ATP measurement should be kept uniform for each animal and completed within 20 minutes. The luciferin/luciferase method is applied to measure the bioluminescence in Relative Luminescence Units (RLU). This study includes: measurements (weighing, RLU), and clinical daily observations. The results are expressed as the Stimulation Index (SI) obtained by calculation. The SI should be ¡Ý1.8 before further evaluation of the test material as a potential skin sensitizer is warranted.


Book
Les nodules polymétalliques dans les grands fonds océaniques : une extraordinaire aventure minière et scientifique sous-marine
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ISBN: 9782711771660 2711771660 Year: 2008 Publisher: Paris : Société géologique de France : Vuibert,

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The Indian Ocean nodule field : geology and resource potential
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ISBN: 9780444637628 0444637621 0128054743 9780128054741 Year: 2018 Publisher: Amsterdam, Netherlands : Elsevier,

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The Indian Ocean Nodule Field: Geology and Resource Potential, Second Edition, provides a view of the most recent studies along with classical theories starting from the 1960s. New concepts, hypotheses and critical appreciation of the state-of-the-art knowledge on nodule formation and resource management are featured in this new edition. The economy of the Indian Ocean has vast potential and yet it is one of the least studied oceans. Garnering economic advantage out of mineral resources from deep oceans has been a long cherished dream for the mining community. The availability of 5-metal rich polymetallic (manganese) nodules in specific areas of the Indian Ocean has lured researchers to discover more about their processes of formation, growth, distribution and enrichment. Collates, discusses and succinctly presents voluminous and scattered information from various books and journals for easy understanding and disseminationFeatures two new chapters: statistical modeling of the formation, growth, distribution and metal enrichment of nodules; and biogeochemistry of the Central Indian Ocean BasinIncludes an assessment of Indian Ocean nodules, one of the least studied oceans in comparison to samples analyzed from the world's oceans.


Dissertation
Genetic and biochemical analysis of the flagellation of Azospirillum brasilense Sp7.
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Year: 1996 Publisher: Leuven : KUL, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen,

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