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PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
Molecular biology --- Polymerase Chain Reaction --- Polymerase chain reaction --- Réaction en chaîne de la polymérase --- methods --- Methodology --- Méthodologie --- Electronic books. -- local. --- Polymerase chain reaction -- Methodology. --- Polymerization -- Methodology. --- Nucleic Acid Amplification Techniques --- Genetic Techniques --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- -57.088 --- 577.2 --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- methods. --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Molecular bases of life. Molecular biology --- Methodology. --- 577.2 Molecular bases of life. Molecular biology --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Réaction en chaîne de la polymérase --- Méthodologie --- 57.088 --- Polymerisation --- Polymers --- Polymers and polymerization --- Synthesis of polymers --- Chemical reactions --- Synthesis --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Polymerase chain reaction - Methodology --- Polymerase Chain Reaction - methods
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PCR is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. PCR: A Practical Approach Volume 2 is not a revised version of PCR:A Practical Approach, but sets out to address some of the new and exciting applications of PCR including direct sequencing, mRNA quantitation and expression, genomic DNA mapping, and mutation analysis.
General biochemistry --- DNA (deoxyribonucleic acid) --- biochemie --- PCR (polymerase chain reaction) --- genetische informatie --- Molecular biology --- Analytical biochemistry --- biologie --- moleculaire biologie --- polymeren --- Macromolecules --- Gene Amplification --- Réaction en chaîne de la polymérase --- Technique des traceurs --- Tracer techniques --- État physique --- physical states --- Polymerase Chain Reaction. --- -Chain reaction, Polymerase --- PCR (Biochemistry) --- DNA polymerases --- Amplification, Gene --- Anchored Polymerase Chain Reaction --- Inverse Polymerase Chain Reaction --- Nested Polymerase Chain Reaction --- Anchored PCR --- Inverse PCR --- Nested PCR --- PCR, Anchored --- PCR, Inverse --- PCR, Nested --- Polymerase Chain Reactions --- Reaction, Polymerase Chain --- Reactions, Polymerase Chain --- Ligase Chain Reaction --- -Molecular bases of life. Molecular biology --- -577.2 Molecular bases of life. Molecular biology --- Chain reaction, Polymerase --- Réaction en chaîne de la polymérase --- Polymerase chain reaction --- DNA Replication --- Polymerase Chain Reaction --- 577.2 --- 577.2 Molecular bases of life. Molecular biology --- Molecular bases of life. Molecular biology --- PCR --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Laboratory manuals --- #WSCH:WBIO --- Polymerization --- methods --- Laboratory manuals. --- Polymerase chain reaction. --- Dna replication. --- Gene amplification. --- DNA Replication. --- Gene Amplification. --- klonen --- enzymen --- Polymerase chain reaction - Laboratory manuals. --- Genes
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