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Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Analytical biochemistry --- Gene expression --- Polymerase chain reaction --- Reverse transcriptase --- Research --- Methodology --- Biochemical Processes --- Nucleic Acid Amplification Techniques --- Gene Expression --- Chemical Processes --- Genetic Techniques --- Biochemical Phenomena --- Genetic Processes --- Chemical Phenomena --- Investigative Techniques --- Genetic Phenomena --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Polymerase Chain Reaction --- Transcription, Genetic --- Biology --- Health & Biological Sciences --- Genetics --- Cell biology. --- Biochemistry. --- Cell Biology. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Cell biology --- Cellular biology --- Cells --- Composition --- Gene expression - Research - Methodology