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The regulation of eukaryotic gene expression is a fundamental and highly active area of current research. This work brings together leading workers in the field for the first time to review the latest findings on all major aspects of transcription and processing, and their regulation. Knowledge of these events is increasing rapidly, with great significance for all those working in molecular biology, making this volume a timely source of invaluable information.
Molecular biology --- RNA Splicing --- Genetic transcription --- RNA splicing --- Eukaryotic cells --- Transcription génétique --- Epissage --- Cellules eucaryotes --- Transcription, Genetic --- Eukaryotic cells. --- RNA splicing. --- RNA Splicing. --- Transcription, Genetic. --- Basic Sciences. Molecular Biology -- Nucleic Acids --- Basic Sciences Molecular Biology -- Nucleic Acids. --- Rna splicing. --- Transcription, genetic. --- Basic sciences molecular biology -- nucleic acids. --- Genetic transcription.
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Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Electrophoresis, Polyacrylamide gel --- Immunoelectrophoresis --- Electrophoresis, Polyacrylamide Gel --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- isolation & purification --- Electrophoresis, Polyacrylamide Gel. --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Polyacrylamide gel electrophoresis. --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- Proteins. --- 577.112 --- 577.112 Proteins --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- 57.088 --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Analytical biochemistry --- Eiwitten. --- Elektroforese. --- methods --- laboratory manuals. --- Protein --- Electrophoresis, Polyacrylamide Gel - laboratory manuals --- Proteins - laboratory manuals
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The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2, covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.
Molecular biology --- DNA probes. --- Sondes ADN --- Basic Sciences. Genetics --- Genetics (General) --- Genetics (General). --- GENES --- MOLECULAR BIOLOGY --- PROBES --- TECHNIQUES --- LABORATORY MANUALS --- USES
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Research progress on the molecular biology of the immune system has been particularly rapid. This unique volume brings together five reviews by leading researchers on all major aspects of work in this area. These exciting advances have major implications not only for immunologists, but for all researchers studying gene structure and function.
Immunochemistry --- Molecular Biology --- Immunochemistry. --- Immunology. Immunopathology --- Molecular Biology.
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PCR is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. PCR: A Practical Approach Volume 2 is not a revised version of PCR:A Practical Approach, but sets out to address some of the new and exciting applications of PCR including direct sequencing, mRNA quantitation and expression, genomic DNA mapping, and mutation analysis.
General biochemistry --- DNA (deoxyribonucleic acid) --- biochemie --- PCR (polymerase chain reaction) --- genetische informatie --- Molecular biology --- Analytical biochemistry --- biologie --- moleculaire biologie --- polymeren --- Macromolecules --- Gene Amplification --- Réaction en chaîne de la polymérase --- Technique des traceurs --- Tracer techniques --- État physique --- physical states --- Polymerase Chain Reaction. --- -Chain reaction, Polymerase --- PCR (Biochemistry) --- DNA polymerases --- Amplification, Gene --- Anchored Polymerase Chain Reaction --- Inverse Polymerase Chain Reaction --- Nested Polymerase Chain Reaction --- Anchored PCR --- Inverse PCR --- Nested PCR --- PCR, Anchored --- PCR, Inverse --- PCR, Nested --- Polymerase Chain Reactions --- Reaction, Polymerase Chain --- Reactions, Polymerase Chain --- Ligase Chain Reaction --- -Molecular bases of life. Molecular biology --- -577.2 Molecular bases of life. Molecular biology --- Chain reaction, Polymerase --- Réaction en chaîne de la polymérase --- Polymerase chain reaction --- DNA Replication --- Polymerase Chain Reaction --- 577.2 --- 577.2 Molecular bases of life. Molecular biology --- Molecular bases of life. Molecular biology --- PCR --- Replication, Autonomous --- Autonomous Replication --- Autonomous Replications --- DNA Replications --- Replication, DNA --- Replications, Autonomous --- Replications, DNA --- DNA-Binding Proteins --- Virus Replication --- S Phase --- Laboratory manuals --- #WSCH:WBIO --- Polymerization --- methods --- Laboratory manuals. --- Polymerase chain reaction. --- Dna replication. --- Gene amplification. --- DNA Replication. --- Gene Amplification. --- klonen --- enzymen --- Polymerase chain reaction - Laboratory manuals. --- Genes
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This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
Biological techniques --- Biochemical engineering --- Molecular biology --- Molecular cloning --- Recombinant DNA --- Laboratory manuals. --- Cloning, Molecular --- DNA, Recombinant --- Clonage moléculaire --- laboratory manuals. --- Laboratory manuals --- Manuels de laboratoire --- Molecular cloning - Laboratory manuals. --- Recombinant DNA - Laboratory manuals.
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