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This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during ‘physiological’ resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.

Immunology. --- Immunobiology --- Life sciences --- Serology --- Cèl·lules T --- Motilitat cel·lular --- Moviment cel·lular --- Fisiologia cel·lular --- Cèl·lules timodependents --- Limfòcits T --- Limfòcits timodependents --- Timòcits --- Limfòcits

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Stem cells --- Therapeutic use. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Cèl·lules mare --- Teràpia cel·lular --- Citoteràpia --- Histoteràpia --- Terapèutica cel·lular --- Ús terapèutic de les cèl·lules --- Organoteràpia --- Trasplantament d'òrgans --- Cèl·lules --- Cèl·lules mare embrionàries

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343.81 --- Gevangeniswezen. Penitentiaire inrichting. Gevangenis. Cel. Dwangarbeid --- 343.81 Gevangeniswezen. Penitentiaire inrichting. Gevangenis. Cel. Dwangarbeid

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This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. .

Cèl·lules K --- Mastòcits --- Cèl·lules encebades --- Labròcits --- Histamina --- Cèl·lules assassines --- Cèl·lules citocides --- Cèl·lules citocides naturals --- Cèl·lules NK --- Cèl·lules immunocompetents --- Macròfags --- Leucòcits --- Citotoxicitat per mediació cel·lular --- Immunology. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Immunobiology --- Life sciences --- Serology

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This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Immune system. --- Immunotherapy. --- Therapeutic immunology --- Clinical immunology --- Therapeutics --- Immunological system --- Anatomy --- Immunology --- Immunoteràpia --- Sistema immunitari --- Cèl·lules --- Biologia --- Fisiologia --- Reproducció --- Astròcits --- Blastoderma --- Cèl·lules eucariotes --- Cèl·lules canceroses --- Cèl·lules epitelials --- Cèl·lules germinals --- Cèl·lules hepàtiques --- Cèl·lules i teixits vegetals --- Cèl·lules mare --- Cèl·lules musculars --- Cèl·lules sanguínies --- Citoplasma --- Cromatina --- Fecundació --- Fibroblasts --- Histologia --- Irritabilitat --- Mort cel·lular --- Neurones --- Neurones motores --- Patologia cel·lular --- Protoplasts --- Protozous --- Ou (Biologia) --- Ultraestructura (Biologia) --- Citologia --- Clonatge --- Embriologia --- Protoplasma --- Regeneració (Biologia) --- Sistema immunològic --- Anatomia --- Immunologia --- Cèl·lules immunocompetents --- Medul·la òssia --- Sistema limfàtic --- Terapèutica immunològica --- Immunologia clínica --- Terapèutica --- Adjuvants immunològics --- Seroteràpia --- Timosina --- Immunology. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Immunobiology --- Life sciences --- Serology