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Bioconjugates --- Biochemistry --- Proteins --- Peptides --- Nucleic Acids --- Synthesis. --- methods. --- chemistry. --- Basic Sciences. Chemistry --- Organic Chemistry. --- #WDIR:vfm2 --- ELSEVIER-B EPUB-LIV-FT --- Bioconjugate chemistry --- Conjugated biomolecules --- Conjugation biochemistry --- Conjugation chemistry --- Biomolecules --- Metabolic conjugation --- Synthesis --- Methods. --- Chemistry. --- Bioconjugates - Synthesis. --- Biochemistry - methods. --- Proteins - chemistry. --- Peptides - chemistry. --- Nucleic Acids - chemistry.

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Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.

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Recent major advances in our understanding of modulating protein functions has led to the development of new methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. Other topics of discussion include: design of structural elementary motifs, entire proteins, and interfaces of protein complexes, and of amyloidogenic polypeptides and amyloid inhibitors. Authoritative and cutting-edge, Protein Design: Methods and Applications will be of major interest to protein scientists, biochemists, and all experimentalists selecting the strategy most adaptable for their design problem.

Protein engineering --- Protein Engineering --- Proteins --- Protein Conformation --- Protein Folding --- methods --- chemistry --- Protein engineering. --- Biophysical Processes --- Biophysical Phenomena --- Engineering, Protein --- Protein design --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biochemical engineering --- Genetic engineering --- Design --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- 577.122 --- 577.122 Protein metabolism --- Protein metabolism --- Protein Folding, Globular --- Folding, Globular Protein --- Folding, Protein --- Foldings, Globular Protein --- Foldings, Protein --- Globular Protein Folding --- Globular Protein Foldings --- Protein Foldings --- Protein Foldings, Globular --- Proteostasis --- Protein Multimerization --- Intrinsically Disordered Proteins --- Conformation, Protein --- Conformations, Protein --- Protein Conformations --- Protein Engineering - methods --- Proteins - chemistry