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How to design, execute, and interpret experiments for protein sequencing using mass spectrometryThe rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation.Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99uccess rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes:* Peptide fragmentation and interpretation of product ion spectra* Basic polyacrylamide gel electrophoresis* Preparation of protein digests for sequencing experiments* Mass spectrometric analysis using capillary liquid chromatography* Techniques for protein identification by database searches* Characterization of modified peptides using tandem mass spectrometryAnd much more

Spectrometric and optical chemical analysis --- Organic spectroscopy --- Mass spectrometry. --- Nucleotide sequence. --- Proteins --- Analysis. --- Mass spectrometry --- Nucleotide sequence --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Analysis

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Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.

Nucleotide sequence --- Nucleotides --- Analysis. --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleic acids --- Sequence alignment (Bioinformatics) --- Analysis --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

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It is widely recognized that analytical technologies and techniques are playing a pioneering role in a range of today's foremost challenging scientific endeavours, including especially biological and biomedical research. Worthy of mention, for example, are the role that high performance separation techniques played in mapping the human genome and the pioneering work done within mass spectrometry. It is also apparent that state-of-the-art pharmaceutical and biomedical research is the major driving force of the development of new analytical techniques. Advancements in genomics research h

Proteins --- Nucleotide sequence. --- Chromatographic analysis. --- Mass spectrometry. --- Analysis. --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Analysis, Chromatographic --- Chromatography --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Chemistry, Analytic --- Phase partition --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Analysis --- Analytical chemistry

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Since the independent invention of DNA sequencing by Sanger and by Gilbert 30 years ago, it has grown from a small scale technique capable of reading several kilobase-pair of sequence per day into today's multibillion dollar industry. This growth has spurred the development of new sequencing technologies that do not involve either electrophoresis or Sanger sequencing chemistries. Sequencing by Synthesis (SBS) involves multiple parallel micro-sequencing addition events occurring on a surface, where data from each round is detected by imaging. New High Throughput Technologies for DNA Se

Nucleotide sequence --- Genomics --- Genetic engineering --- Technological innovations. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Genome research --- Genomes --- Molecular genetics --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Research --- Analysis

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Human genetics --- DNA --- Nucleotide sequence. --- Genetic disorders. --- Variation. --- Analysis. --- Diseases --- Medical genetics --- Congenital diseases --- Disorders, Genetic --- Disorders, Inherited --- Genetic diseases --- Hereditary diseases --- Inherited diseases --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Variation (Biology) --- Genetic variation in humans --- Analysis