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Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro­ teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech­ nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato­ ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom­ ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.

Proteins --- Protéines --- isolation & purification --- laboratory manuals --- Purification --- Laboratory manuals --- Manuels de laboratoire --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- 57.088.3 --- 577.112 --- -Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Methods and techniques for separation,fractionation and purification. --- -Laboratory manuals --- Laboratory manuals. --- -Methods and techniques for separation,fractionation and purification. --- 577.112 Proteins --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- -Biomolecules --- Proteids --- -577.112 Proteins --- Protéines --- Purification&delete& --- Methods and techniques for separation,fractionation and purification --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- laboratory manuals. --- Cytology. --- Proteins. --- Protéines.

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This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.

Analytical biochemistry --- Isoelectric focusing. --- Analytical biochemistry. --- Analytic biochemistry --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Biochemistry --- Electrofocusing --- Focusing, Isoelectric --- Chemistry, Organic --- Electrophoresis --- Qualitative --- Isoelectric focusing --- 57.088.3 --- 57.08 --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- Methods and techniques for separation,fractionation and purification. --- 57.08 Biological techniques. Experimental methods. Equipment --- Biological techniques. Experimental methods. Equipment

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In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.

Animal protein --- Protéine végétale --- Plant protein --- Extraction --- Purification --- Proteolysis --- Enzymes --- Chromatographie --- Chromatography --- Électrophorèse --- Electrophoresis --- Biomolecules. --- Electronic books. -- local. --- Proteins -- Purification -- Laboratory manuals. --- Proteins --- Publication Formats --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Publication Characteristics --- Laboratory Manuals --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- 577.112.083 --- 57.088.3 --- 577.112 --- -Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Proteins--?.083 --- Methods and techniques for separation,fractionation and purification. --- -Laboratory manuals --- -Proteins--?.083 --- 577.112 Proteins --- 57.088.3 Methods and techniques for separation,fractionation and purification. --- 577.112.083 Proteins--?.083 --- -577.112 Proteins --- Proteids --- Methods and techniques for separation,fractionation and purification --- Biological molecules --- Molecules --- Molecular biology --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition