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Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be d
Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Escherichia coli. --- E. coli (Bacterium) --- Escherichia
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The title of the book ""The Universe of Escherichia coli"" aims to present and emphasize the huge diversity of this bacterial species and our efforts to prevent the E. coli infections. As it is part of the gut microbiota, E. coli is a well-known commensal species, and probiotic E. coli strains are successfully used for improving host's health. Also many ""workhorse"" E. coli strain exist that are employed in laboratory and biotechnology settings. But certain E. coli strains can cause intestinal and also extraintestinal infections at many anatomical sites. Therefore many efforts are undertaken to prevent E. coli infections, among them food safety, vaccines, but also new antimicrobial agents are searched for.
Escherichia coli. --- E. coli (Bacterium) --- Escherichia --- Life Sciences --- Microbiology --- Genetics and Molecular Biology --- Biochemistry --- Bacteriology
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Molecular biology --- General microbiology --- Escherichia coli --- Salmonella typhimurium --- Escherichia Coli --- Salmonella Typhimurium --- Escherichia coli. --- Salmonella typhimurium. --- #abib:almm --- Bacillus typhimurium --- Salmonella --- E. coli (Bacterium) --- Escherichia --- Salmonella typhimurium LT2 --- Diffusely Adherent E. coli --- Enteroaggregative E. coli --- Enteroinvasive E. coli --- Enteroinvasive Escherichia coli --- Alkalescens-Dispar Group --- Diffusely Adherent Escherichia coli --- E coli --- EAggEC --- Enteroaggregative Escherichia coli --- Escherichia coli Proteins --- Escherichia Coli. --- Salmonella Typhimurium. --- Biology --- Microbiology & Immunology --- Health & Biological Sciences --- Salmonella tyhimurmium --- Escherichian coli --- Monograph --- Bacillus coli --- Bacterium coli --- Bacterium coli commune --- Enterococcus coli
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The 2e of Escherichia coli is a unique, comprehensive analysis of the biology and molecular mechanisms that enable this ubiquitous organism to thrive. Leading investigators in the field discuss the molecular basis of E. coli pathogenesis followed by chapters on genomics and evolution. Detailed descriptions of distinct strains reveal the molecular pathogenesis of each and the causes of intestinal and extra-intestinal infections in humans. This work concludes with a presentation of virulence factors common to two or more pathotypes. The book is a great resource for references and up-to-date know
Escherichia coli infections. --- Escherichia coli. --- Virulence (Microbiology) --- Microbial virulence --- Pathogenic microorganisms --- E. coli (Bacterium) --- Escherichia --- Colibacillosis --- Gram-negative bacterial infections
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Escherichia coli is a facultative anaerobic Gamma-proteobacterium, which belongs to the family Enterobacteriaceae. While being an important constituent of the normal gut microbiota, specialized E. coli clones have acquired genetic elements that allow them to compete with the endogenous commensals, colonise normally sterile niches and cause disease. E. coli pathotypes can cause intestinal and extra intestinal infections (e.g. UTI, sepsis) and associate with mammalian cells while being extra- or intra-cellular. In recent years, E. coli infections have become a serious clinical problem, due to the rapid spread of antibiotic resistance. Thus, infections with intestinal E. coli (e.g. E. coli O104) or extraintestinal pathogenic strains (e.g. E. coli ST131) are becoming difficult to treat and are often lethal. Consequently, there is a pressing need to develop alternative control measures, including the identification of new drug targets and development of vaccines that offer lasting protection. This volume focuses on several types of E. coli infections (intestinal and extraintestinal), virulence factors, and E. coli pandemics. It addresses the problem of antibiotic resistance, and a dedicated chapter discusses the need to develop alternative control measures. Given its depth and breadth of coverage, the book will benefit all those interested in the biology, genetics, physiology and pathogenesis of E. coli, and in related vaccine development.
Escherichia coli. --- E. coli (Bacterium) --- Escherichia --- Microbiology. --- Emerging infectious diseases. --- Bacteriology. --- Medical Microbiology. --- Infectious Diseases. --- Microbiology --- Emerging infections --- New infectious diseases --- Re-emerging infectious diseases --- Reemerging infectious diseases --- Communicable diseases --- Microbial biology --- Biology --- Microorganisms --- Medical microbiology. --- Infectious diseases.
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DescriptionAlthough most strains of E. coli bacteria are harmless and live in the intestines of healthy humans and animals, several strains can produce powerful toxins and cause severe illness in humans. This versatile pathogen is best known for being transmitted to humans through contaminated foods - such as undercooked meat and unpasteurized fruit juice - and has attracts much attention when serious outbreaks occur.Importance of TopicE. coli is capable of causing a wide variety of diseases - from urinary tract infections to meningitis. A considerable amount of m
Escherichia coli infections. --- Escherichia coli. --- Virulence (Microbiology) --- Microbial virulence --- Pathogenic microorganisms --- E. coli (Bacterium) --- Escherichia --- Colibacillosis --- Gram-negative bacterial infections --- ESCHERICHIA COLI --- ADHESINS, ESCHERICHIA COLI --- ANTIGENS, BACTERIAL --- SHIGA TOXINS --- HEMOLYSINS --- GENETICS --- PATHOGENICITY --- ULTRASTRUCTURE --- CLASSIFICATION
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579.842.11 --- 579.67 --- Escherichia coli --- Food --- -#abib:almm --- 579.2 --- 579.63 --- 579.84 --- 616-093 --- 616.98 --- E. coli --- bacteriën --- biologie --- geneeskunde --- gramnegatieve bacterien --- groeicurven --- hygiëne --- industriële microbiologie --- infectieziekten --- microbiologie --- parasitologie --- pathologie --- recht (wetgeving) --- testmethoden --- voeding --- voedingsmiddelen --- ziekten --- Foods --- Dinners and dining --- Home economics --- Table --- Cooking --- Diet --- Dietaries --- Gastronomy --- Nutrition --- E. coli (Bacterium) --- Escherichia --- Food microbiology --- Microbiology --- algemene microbiologie --- hygiënische microbiologie --- gram-negative bacteria --- Escherichia coli. --- Microbiology. --- 579.67 Food microbiology --- 579.842.11 Escherichia --- #abib:almm --- Sanitary microbiology --- Bacteriology
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Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.
genomically engineered E. coli --- n/a --- in vitro transcription-translation (TX-TL) --- tPa --- tissue plasminogen activator --- in vitro translation --- enzymes --- DNA origami --- antimicrobials --- cell-free metabolic engineering (CFME) --- cell-free transcription-translation --- in vitro protein synthesis --- cell-free metabolic engineering --- E. coli crude extract preparation --- chaperones --- ischemic stroke --- microsomes --- sonication --- cell-free protein synthesis --- growth factors --- artificial cell --- unnatural protein --- cell-free synthetic biology --- rapid prototyping --- drug development --- unnatural amino acid --- eukaryotic lysates --- cell-free protein expression (CFPE) --- riboregulator --- synthetic biology --- mathematical model --- CFPS --- cell-free protein synthesis (CFPS) --- bioconversion platform --- colicins --- protein production
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Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
Plasmids --- Escherichia coli --- Genetic vectors --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- E. coli (Bacterium) --- Escherichia --- Paragenes --- Cytoplasmic inheritance --- Mobile genetic elements --- Extrachromosomal DNA --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition
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Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics.
Escherichia coli --- Gene expression --- Genetics --- E. coli (Bacterium) --- Escherichia --- Genes --- Genetic regulation --- Expression --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Escherichia coli - Genetics
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