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DNA-protein interactions. --- RNA-protein interactions.

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The pivotal role played by transcription factors in regulating gene expression has ensured that their study is a major area of current research activity. This book provides a comprehensive description of all the approaches and methods required to identify, isolate and characterize transcription factors. Starting with a specific DNA sequence, these protocols allow the detection and identification of proteins that bind to it and detailed analysis of the DNA binding site. This is followed by procedures for the functional assay of transcription factors and their purification. Also included are methods for the characterization of transcription factor genes, including DNA cloning. The analysis of transcription factors is of central interest to all molecular biologists involved in studying gene expression, making this book an important text for a wide range of researchers from postgraduate students to established scientists.

Transcription Factors --- DNA-protein interactions --- Genetic regulation --- Transcription factors --- Régulation génétique --- Facteurs de transcription --- physiology --- DNA-protein interactions. --- Genetic regulation. --- Transcription factors. --- physiology. --- Régulation génétique

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Molecular biology --- DNA --- Bacterial Proteins --- RNA --- DNA-protein interactions --- ultrastructure --- Basic Sciences. Chemistry --- DNA-protein interactions. --- DNA-BINDING PROTEINS --- Eukaryotic cells --- Rna polymerases --- Transcription --- Biochemistry --- Proteins and Enzymes --- genetic --- DNA-BINDING PROTEINS. --- Eukaryotic cells. --- Rna polymerases. --- Proteins and Enzymes. --- genetic. --- Basic sciences. chemistry --- Dna-binding proteins. --- Dna-protein interactions. --- Proteins and enzymes. --- Genetic. --- Biochemie. --- DNA - ultrastructure --- RNA - ultrastructure

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DNA-protein interactions. --- Eukaryotic Cells. --- Eukaryotic cells. --- Gene Expression Regulation. --- Genetic regulation. --- Genetic transcription --- Transcription Factors. --- Transcription factors. --- Transcription, Genetic. --- Regulation.

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DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.

DNA-protein interactions --- DNA-protein interactions. --- Electronic books. -- local. --- Protein binding. --- Nuclease Protection Assays --- Proteins --- Genetic Techniques --- Amino Acids, Peptides, and Proteins --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- DNA Footprinting --- DNA-Binding Proteins --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology.