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This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science.

Proteins. --- Cytology—Technique. --- Cytological Techniques. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics

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Biological techniques --- Histology. Cytology --- Chromosomes --- Cytological Technics --- Cytology --- Cytologie --- Technique --- CYTOLOGICAL TECHNIQUES --- CHROMOSOMES --- chromosomes --- Code génétique --- genetic code --- Chromosomes. --- Cytological Techniques. --- 575'3 --- 576.316 --- 57.086 --- Cytologic Technics --- Cytological Technic --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Chromosome --- methods --- Cytology - Technique --- Basic Sciences. Genetics --- Cytogenetics --- Cytology - Technique. --- Cytogenetics.

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Cytochalasins --- Cytology --- Physiological effect --- Technique --- -Cytology --- -Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Antibiotics --- Heterocyclic compounds --- Mycotoxins --- Physiological effects --- Cytochalasins. --- Physiological effect. --- Technique. --- -Physiological effects --- Cytochalasins - Physiological effect --- Cytology - Technique

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This book explores the regenerative properties of fetal stem cells, from feto-maternal cell traffic through perinatal stem cells, with a discussion of key topics including stem cell banking, drug screening, in utero stem cell transplantation and ethical considerations. The expertly authored chapters also delve into embryonic, amniotic membrane, and umbilical cord blood stem cells; fetal development models; fetal cell reprogramming; culture methods; disease models; perinatal gene therapy, and more. These chapters are grouped into four sections, each discussing a separate prenatal stem cell population and providing fascinating historical contexts for our knowledge of these systems. Featuring a foreword written by the renowned Dr. Joseph Vacanti of the Harvard Stem Cell Institute, Fetal Stem Cells in Regenerative Medicine: Principles and Translational Strategies is a welcome and timely contribution to the Stem Cell Biology and Regenerative Medicine series. It is essential reading for scientists and researchers, clinicians and residents, and advanced students involved in stem cells, regenerative medicine, tissue engineering, and related disciplines such as embryology.

Life sciences. --- Stem cells. --- Cell culture. --- Regenerative medicine. --- Tissue engineering. --- Embryology. --- Life Sciences. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Cell Culture. --- Stem cells --- Therapeutic use. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Medicine --- Regeneration (Biology) --- Cells --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Cultures (Biology) --- Cytology --- Embryology --- Development --- Technique --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Cytology—Technique. --- Stem Cell Biology. --- Regenerative Medicine and Tissue Engineering. --- Cytological Techniques.