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MicroRNA: From Bench to Bedside provides an in-depth, expansive overview of microRNA from fundamentals to clinical practice. It presents researchers with detailed insights on the topic of microRNA, exploring foundational knowledge that is followed by methodologies and the latest technologies for research and potential theragnostic and therapeutic applications for specific diseases.

MicroRNA. --- MicroRNA --- Micro RNA --- miRNA (MicroRNA) --- pre-miRNA (Pre-MicroRNA) --- pri-miRNA (Primary MicroRNA) --- Primary MicroRNA --- Primary miRNA --- Small temporal RNA --- stRNA (Small temporal RNA) --- Antisense RNA --- Non-coding RNA --- Health aspects. --- MicroRNAs

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MicroRNA. --- MicroRNAs. --- Micro RNA --- MicroRNA --- Primary MicroRNA --- Primary miRNA --- miRNAs --- pre-miRNA --- pri-miRNA --- RNA, Small Temporal --- Small Temporal RNA --- miRNA --- stRNA --- MicroRNA, Primary --- RNA, Micro --- Temporal RNA, Small --- miRNA, Primary --- pre miRNA --- pri miRNA --- RNA, Small Interfering --- miRNA (MicroRNA) --- pre-miRNA (Pre-MicroRNA) --- pri-miRNA (Primary MicroRNA) --- Small temporal RNA --- stRNA (Small temporal RNA) --- Antisense RNA --- Non-coding RNA

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as riboswitch discovery and valid

Small interfering RNA. --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- Scan RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Antisense RNA

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The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications.

Gene expression regulation --- Gene silencing --- Micrornas --- Rna interference. --- Rna, small interfering --- Small interfering rna --- Genetics. --- Physiology. --- Small interfering RNA --- Gene inactivation --- Inactivation, Gene --- Silencing, Gene --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- Scan RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Genetic regulation --- Antisense RNA --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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In recent years Molecular Biology has experienced an unprecedented revolution by the discovery of functional small RNAs. The number of cellular processes in which non-coding RNAs are involved is growing rapidly and include gene regulation on the transcriptional, post-transcriptional and translational level. To complicate matters, these processes seem to be strongly interconnected on the one hand, and diverse among different organisms on the other. This volume describes strategies for the discovery and validation of small RNAs and provides a snapshot of our current understanding of the different mechanisms triggered by small RNAs.

Small interfering RNA. --- Small interfering RNA --- RNA, Antisense --- RNA, Small Nuclear --- Genetic Processes --- RNA, Small Untranslated --- RNA, Nuclear --- RNA --- Antisense Elements (Genetics) --- RNA, Untranslated --- Genetic Phenomena --- Nucleic Acids --- Nucleic Acids, Nucleotides, and Nucleosides --- Phenomena and Processes --- Chemicals and Drugs --- Gene Expression Regulation --- RNA, Small Nucleolar --- RNA, Small Interfering --- Human Anatomy & Physiology --- Health & Biological Sciences --- Animal Biochemistry --- Biochemistry --- Biology - General --- Biology --- Chemistry --- Physical Sciences & Mathematics --- Antisense RNA. --- Complementary RNA --- piRNA (Piwi-interacting RNA) --- Piwi-interacting RNA --- Piwi protein-interacting RNA --- rasiRNA (Repeat-associated small interfering RNA) --- Repeat-associated siRNA --- Repeat-associated small interfering RNA --- Scan RNA --- scnRNA (Small scan RNA) --- Short hairpin RNA --- Short interfering RNA --- shRNA (Short hairpin RNA) --- siRNA (Small interfering RNA) --- Small hairpin RNA --- Small scan RNA --- tasiRNA (Trans-acting small interfering RNA) --- Trans-acting siRNA --- Trans-acting small interfering RNA --- Life sciences. --- Biochemistry. --- Cell biology. --- Developmental biology. --- Life Sciences. --- Biochemistry, general. --- Cell Biology. --- Developmental Biology. --- Antisense nucleic acids --- Antisense RNA --- Cytology. --- Development (Biology) --- Growth --- Ontogeny --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition --- Physiology. --- Genetics. --- Analysis.