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This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Human genetics. --- RNA Viruses --- Reverse Genetics --- Coronavirus --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- genetics --- methods --- genetics. --- methods.

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Kant in Brazil is a collected volume of essays conceived at the 2005 International Kant Congress in Sao Paulo as a way to make accessible to Anglophone Kant scholars some of the best work on Kant produced by Brazilian scholars. The availability of this material in English for the first time will promote interaction between North American and Brazilian scholars as well as enable Anglophone readers worldwide to incorporate excellent but previously neglected work into their own debates about Kant. The book contains an editor's introduction providing an overview of the institutional structure of Kant studies in Brazil. The essays that follow, translated from Portuguese, include a survey of the history of Kant studies in Brazil over the past two centuries as well as interpretive essays that span the corpus of Kant's work in theoretical philosophy, ethics, political philosophy, history, aesthetics, and teleology. Various styles of philosophy are put into practice as well: analytical, philological, reflective, comparative, displaying the broad and diverse nature of Brazilian philosophy. Frederick Rauscher is associate professor of philosophy at Michigan State University. Daniel Omar Perez is professor of philosophy at the Pontifical Catholic University of Parana, Brazil.

Philosophie --- Philosophy, Brazilian. --- Influence (Literary, artistic, etc.) --- Artistic impact --- Artistic influence --- Impact (Literary, artistic, etc.) --- Literary impact --- Literary influence --- Literary tradition --- Tradition (Literature) --- Art --- Influence (Psychology) --- Literature --- Intermediality --- Intertextuality --- Originality in literature --- Brazilian philosophy --- Kant, Immanuel --- Kant, Immanuel, --- Influence. --- Kant, I. --- Kānt, ʻAmmānūʼīl, --- Kant, Immanouel, --- Kant, Immanuil, --- Kʻantʻŭ, --- Kant, --- Kant, Emmanuel, --- Ḳanṭ, ʻImanuʼel, --- Kant, E., --- Kant, Emanuel, --- Cantơ, I., --- Kant, Emanuele, --- Kant, Im. --- קאנט --- קאנט, א. --- קאנט, עמנואל --- קאנט, עמנואל, --- קאנט, ע. --- קנט --- קנט, עמנואל --- קנט, עמנואל, --- كانت ، ايمانوئل --- كنت، إمانويل، --- カントイマニユエル, --- Kangde, --- 康德, --- Kanṭ, Īmānwīl, --- كانط، إيمانويل --- Kant, Manuel, --- Brazilian Kant scholars. --- Brazilian philosophy. --- Daniel Omar Perez. --- Frederick Rauscher. --- International Kant Congress. --- Kant scholars. --- North American scholars. --- analytical philosophy. --- philosophy in Brazil. --- philosophy.

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Information Technology --- Computer Science (Hardware & Networks)