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This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach.  The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time.  This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.

Agriculture - General --- Plant Sciences --- Agriculture --- Earth & Environmental Sciences --- Botanical microtechnique. --- Plant microtechnique --- Life sciences. --- Plant anatomy. --- Plant development. --- Plant breeding. --- Life Sciences. --- Plant Breeding/Biotechnology. --- Plant Anatomy/Development. --- Plant Systematics/Taxonomy/Biogeography. --- Botanical microscopy --- Botany --- Microtechnique --- Technique --- Plant structure --- Plants --- Structural botany --- Vegetable anatomy --- Anatomy --- Crops --- Breeding --- Structure --- Plant systematics. --- Plant taxonomy. --- Botanical classification --- Botanical systematics --- Botanical taxonomy --- Classification --- Plant biosystematics --- Plant classification --- Plant systematics --- Plant taxonomy --- Systematic botany --- Systematics (Botany) --- Taxonomy, Plant --- Plant taxonomists --- Development of plants --- Plant development --- Developmental biology --- Growth (Plants) --- Ontogeny

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Unraveling the functional properties of structural elements in the brain is one of the fundamental goals of neuroscientific research. In the cerebral cortex this is no mean feat, since cortical areas are defined microstructurally in post-mortem brains but functionally in living brains with electrophysiological or neuroimaging techniques – and cortical areas vary in their topographical properties across individual brains. Being able to map both microstructure and function in the same brains noninvasively in vivo would represent a huge leap forward. In recent years, high-field magnetic resonance imaging (MRI) technologies with spatial resolution below 0.5 mm have set the stage for this by detecting structural differences within the human cerebral cortex, beyond the Stria of Gennari. This provides the basis for an in vivo microanatomical brain map, with the enormous potential to make direct correlations between microstructure and function in living human brains. This book starts with Brodmann’s post-mortem map published in the early 20th century, moves on to the almost forgotten microstructural maps of von Economo and Koskinas and the Vogt-Vogt school, sheds some light on more recent approaches that aim at mapping cortical areas noninvasively in living human brains, and culminates with the concept of “in vivo Brodmann mapping” using high-field MRI, which was introduced in the early 21st century.

Cerebral cortex -- Imaging -- Atlases. --- Human physiology. --- Magnetic resonance imaging. --- Medicine. --- Neurosciences. --- Radiology, Medical. --- Stereotaxic techniques. --- Medicine --- Health & Biological Sciences --- Neurology --- Cerebral cortex --- Brain mapping. --- Microtomy. --- Research --- Technique. --- Physiology. --- Section-cutting (Microscopy) --- Sectioning (Microscopy) --- Connectome mapping --- Mapping of the brain --- Topographic brain mapping --- Brain mantle --- Cortex, Cerebral --- Cortex cerebri --- Mantle of brain --- Pallium (Brain) --- Neuroradiology. --- Biomedicine. --- Human Physiology. --- Brain --- Telencephalon --- Histology --- Microtechnique --- Localization of functions --- Technique --- Clinical radiology --- Radiology, Medical --- Radiology (Medicine) --- Medical physics --- Human biology --- Medical sciences --- Physiology --- Human body --- Neural sciences --- Neurological sciences --- Neuroscience --- Nervous system --- Neuroradiography --- Neuroradiology --- Diseases

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This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, EditorOriginally published in 1999.The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Mounting of microscope specimens. --- Transmission electron microscopy --- Electron microscopy --- Microscope specimens, Mounting of --- Microtechnique --- Technique. --- Acetonitrile (data page). --- Acrylic resin. --- Aldehyde. --- Animal testing. --- Antibody. --- Antigen-antibody interaction. --- Aqueous solution. --- Araldite. --- Autolysis (biology). --- Biochemist. --- Biological activity. --- Biological specimen. --- Biologist. --- Biology. --- Biopsy. --- Carbohydrazide. --- Catalysis. --- Cathode ray. --- Cell (biology). --- Cell culture. --- Cell membrane. --- Chemical formula. --- Chemical polarity. --- Chemistry. --- Chloroplast. --- Cryogenics. --- Curing (chemistry). --- Cyanide. --- Cytochemistry. --- Cytochrome c oxidase. --- Cytoplasm. --- Denaturation (biochemistry). --- Division (military). --- Electromagnetic radiation. --- Electron energy loss spectroscopy. --- Electron microscope. --- Electron tomography. --- Embedding. --- Enzyme catalysis. --- Epoxy. --- Erythrocytes. --- Ethanol. --- Ethylene glycol (data page). --- Ethylene glycol dimethacrylate. --- Ethylene glycol. --- Extraction (chemistry). --- Fixation (histology). --- Fixative (drawing). --- Formaldehyde. --- Formic acid (data page). --- Glutaraldehyde. --- Laboratory. --- Lipid. --- Macrophage (ecology). --- Micrograph. --- Microscope slide. --- Microscopy Society of America. --- Microscopy. --- Mixture. --- Moiety (chemistry). --- Mold. --- Molecular mass. --- Molecular sieve. --- Myoepithelial cell. --- Nuclear warfare. --- Nuclear weapon. --- Nuclear weapons delivery. --- Nucleic acid. --- Organic acid anhydride. --- Organic peroxide. --- Osmium tetroxide. --- Perfusion. --- Phosphate. --- Polymer. --- Polymerization. --- Polypropylene glycol. --- Propylene oxide. --- Protein structure. --- Protein. --- Radical (chemistry). --- Recrystallization (chemistry). --- Recrystallization (metallurgy). --- Resin. --- Ribosome. --- Room temperature. --- Scanning electron microscope. --- Solution. --- Solvent. --- Staining. --- Sterilization (microbiology). --- Tetrahydrofuran (data page). --- Tissue culture. --- Transmission electron microscopy. --- Triethylene glycol. --- Ultrastructure. --- Ultraviolet germicidal irradiation. --- Uranyl acetate. --- Vial. --- Viscosity. --- X-ray photoelectron spectroscopy.