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The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission. These GTPases act as molecular switches and their activities are controlled by a large number of regulatory molecules that affect either GTP loading (guanine nucleotide exchange factors or GEFs) or GTP hydrolysis (GTPase activating proteins or GAPs). In their active state, they interact with a continua

Guanosine Triphosphate. --- Rho GTPases. --- rho GTP-Binding Proteins. --- Rho G proteins --- Rho GTP-binding proteins --- Guanosine triphosphatase. --- G proteins --- Guanosine triphosphatase --- GTP phosphohydrolase --- GTPase --- Guanosine triphosphate phosphohydrolase --- Guanosinetriphosphatase --- Phosphatases

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The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325, 329,

Guanosine triphosphatase. --- ras Proteins. --- ras Proteins --- Monomeric GTP-Binding Proteins --- GTP-Binding Proteins --- Intracellular Signaling Peptides and Proteins --- Peptides --- Carrier Proteins --- GTP Phosphohydrolases --- Proteins --- Amino Acids, Peptides, and Proteins --- Acid Anhydride Hydrolases --- Chemicals and Drugs --- Hydrolases --- Enzymes --- Enzymes and Coenzymes --- Ras proteins. --- G proteins. --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Guanosine triphosphatase --- Guanosine-trifosfatase --- Guanosinetriphosphatase --- Trifosfatase [Guanosine-] --- Triphosphatase [Guanosine] --- rho GTP-Binding Proteins --- Binding Protein --- Binding Proteins --- Transport Proteins --- Protein, Binding --- Proteins, Binding --- Proteins, Carrier --- Proteins, Transport --- G-Protein --- GTP-Binding Protein --- GTP-Regulatory Protein --- Guanine Nucleotide Coupling Protein --- G-Proteins --- GTP-Regulatory Proteins --- Guanine Nucleotide Regulatory Proteins --- G Protein --- G Proteins --- GTP Binding Protein --- GTP Binding Proteins --- GTP Regulatory Protein --- GTP Regulatory Proteins --- Protein, GTP-Binding --- Protein, GTP-Regulatory --- Proteins, GTP-Binding --- Proteins, GTP-Regulatory --- Intracellular Signaling Peptides --- Intracellular Signaling Proteins --- Peptides, Intracellular Signaling --- Proteins, Intracellular Signaling --- Signaling Peptides, Intracellular --- Signaling Proteins, Intracellular --- P21 (rho)Protein --- rho GTP-Binding Protein --- rho Protein P21 --- rho G-Proteins --- rho GTPases --- rho Small GTP-Binding Proteins --- G-Proteins, rho --- GTP-Binding Protein, rho --- GTP-Binding Proteins, rho --- GTPases, rho --- P21, rho Protein --- rho G Proteins --- rho GTP Binding Protein --- rho GTP Binding Proteins --- rho Small GTP Binding Proteins --- GTP Phosphohydrolase --- GTPase --- GTPases --- Guanosine Triphosphate Phosphohydrolases --- Guanosinetriphosphatases --- Phosphohydrolase, GTP --- Phosphohydrolases, GTP --- Phosphohydrolases, Guanosine Triphosphate --- Triphosphate Phosphohydrolases, Guanosine --- ras-Related G-Proteins --- ras-Related GTP-Binding Proteins --- G-Proteins, Monomeric --- GTP-Binding Proteins, Monomeric --- Monomeric G-Proteins --- Small G-Proteins --- Small GTPases --- ras-Related GTPases --- G Proteins, Monomeric --- G-Proteins, Small --- G-Proteins, ras-Related --- GTP Binding Proteins, Monomeric --- GTP-Binding Proteins, ras-Related --- GTPases, Small --- GTPases, ras-Related --- Monomeric G Proteins --- Monomeric GTP Binding Proteins --- Proteins, ras-Related GTP-Binding --- Small G Proteins --- ras Related G Proteins --- ras Related GTP Binding Proteins --- ras Related GTPases --- Anhydride Hydrolases, Acid --- Hydrolases, Acid Anhydride --- Biochemistry -- Periodicals. --- Biochemistry -- Technique. --- Enzymes -- Collections. --- Enzymes -- Periodicals. --- Enzymologie -- Technique -- Collections. --- GTP Phosphohydrolases. --- GTP phosphohydrolase --- Guanosine triphosphate phosphohydrolase --- GTP phosphohydrolases. --- Cytoplasmic Vesicles --- Physiological effect --- G proteins --- Membrane proteins --- Phosphatases --- Coenzymes and Enzymes --- Biocatalysts --- Molecular Mechanisms of Pharmacological Action --- Protein Binding --- Receptors, Transferrin --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Polypeptides --- Biocatalyst --- Enzyme --- Carrier Protein --- Transport Protein --- Protein, Carrier --- Protein, Transport --- Protein --- Peptide --- Polypeptide --- Hydrolase --- Small GTPase --- GTPase, Small --- rho GTPase --- GTPase, rho --- Gene expression. --- Gtp phosphohydrolases --- Map kinase signaling system --- Adp-ribosylation factors --- Gtp-binding proteins --- Gtp phosphohydrolase activators --- Gtp-binding protein regulators

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Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.

Cellular signal transduction --- G proteins --- Cell receptors --- GTP-Binding Proteins --- Signal Transduction --- analysis --- physiology --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cellular signal transduction - Laboratory manuals --- G proteins - Laboratory manuals --- Cell receptors - Laboratory manuals --- GTP-Binding Proteins - analysis - laboratory manuals --- Signal Transduction - physiology - Laboratory Manuals

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G Proteins --- G Proteïnen --- Guanosine triphosphatase --- Guanosine-trifosfatase --- Guanosinetriphosphatase --- Proteins [G ] --- Proteïnen [G ] --- Protéines G --- Trifosfatase [Guanosine-] --- Triphosphatase [Guanosine] --- Physiological effect --- Protéines G monomériques --- GENE EXPRESSION --- RECOMBINANT PROTEINS --- PROTEIN PROCESSING, POST-TRANSLATIONAL --- TRANSLATION, GENETIC --- GUANINE NUCLEOTIDES --- CHEMICAL ACTIONS --- PREPARATION --- Gtp phosphohydrolases --- Gtp-binding proteins --- Oncogene protein p21(ras) --- Ras genes --- Guanine nucleotides

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The G protein-coupled receptors (GPCRs), known to be one of the largest superfamilies in the human genome, underpin a multitude of physiological processes and represent a significant target for bioactive and drug discovery programs. In G Protein-Coupled Receptors in Drug Discovery: Methods and Protocols, experts in the field provide a range of classic and cutting-edge laboratory protocols developed for GPCR research, as well as several current review articles relevant to the area of high throughput GPCR screening, with discussion on GPCR structure and GPCR signalling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, G Protein-Coupled Receptors in Drug Discovery: Methods and Protocols serves to aid all those who wish to further the vital research in their GPCR drug discovery programs.

Molecular biology --- Computational Biology. --- Sequence Analysis, DNA. --- Base Sequence. --- Sequence Analysis, RNA. --- Genetics --- Bioinformatics. --- Nucleotide sequence. --- Amino acid sequence. --- Génétique --- Bio-informatique --- Séquence nucléotidique --- Séquence des acides aminés --- Data processing. --- Informatique --- Bioinformatics --- Amino acid sequence --- Data processing --- -Nucleotide sequence --- #TELE:SISTA --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Sequence, Nucleotide --- Nucleic acids --- Nucleotides --- Sequence alignment (Bioinformatics) --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Bio-informatics --- Biological informatics --- Information science --- Computational biology --- Systems biology --- Amino acid sequence analysis --- Analysis, Amino acid sequence --- Peptide sequence --- Peptide sequencing --- Protein sequence --- Protein sequencing --- Sequence, Amino acid --- Amino acids --- Proteins --- Analysis --- Génétique --- Séquence nucléotidique --- Séquence des acides aminés --- Nucleotide sequence --- 57.087 --- 57.087 Methods and techniques for parameter estimation. Recording of biological data --- Methods and techniques for parameter estimation. Recording of biological data --- G proteins --- Drug development --- Receptors, G-Protein-Coupled --- Drug Discovery --- 577.122 --- 577.122 Protein metabolism --- Protein metabolism --- Drug Prospecting --- Discovery, Drug --- Prospecting, Drug --- High-Throughput Screening Assays --- Bioprospecting --- G Protein Coupled Receptor --- G-Protein-Coupled Receptor --- G-Protein-Coupled Receptors --- G Protein Coupled Receptors --- Receptor, G-Protein-Coupled --- Receptors, G Protein Coupled --- Development of drugs --- Drugs --- New drug development --- Pharmacology --- Pharmacy --- GTP-binding proteins --- GTP regulatory proteins --- Guanine nucleotide-binding proteins --- Guanine nucleotide regulatory proteins --- Membrane proteins --- Receptors --- Development --- Toxicology. --- Pharmacology/Toxicology. --- Chemicals --- Medicine --- Poisoning --- Poisons --- Toxicology --- Genetics - Data processing