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Histology. Cytology --- Microbial biotechnology --- Recombinant proteins --- Cells --- Recombinant Proteins --- Cell Culture --- Genetics, Microbial --- Biotechnology --- Biotechnologie microbienne --- Protéines recombinantes --- Periodicals --- Synthesis --- microbiology --- Periodicals. --- utilization --- Périodiques --- Synthèse --- Microbial biotechnology. --- Synthesis. --- Recombinant Proteins. --- Culture Techniques --- Genetics, Microbial. --- Biotechnology. --- microbiology. --- utilization. --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Genetically engineered proteins --- Proteins --- Microorganisms --- Biotechnologies --- Genetic engineering --- applied microbiology --- Virus Physiological Phenomena --- Bacterial Physiological Phenomena --- Genome, Microbial --- Bioreactors --- Recombinant molecules --- Industrial microbiology --- Biotechnological microorganisms --- Histology. --- Microbiology. --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Diffusion Chambers, Culture --- General microbiology --- statistics & numerical data. --- Bioengineering --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant --- Biotecnologia microbiana --- Genètica microbiana --- Proteïnes recombinants

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Plant biotechnology --- Botanical chemistry --- Microbial biotechnology --- Biochemistry --- Genetic Engineering --- Genetics, Microbial --- Molecular Biology --- Plants --- Recombinant Proteins --- genetics --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Plant --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- Microbial Genetics --- Genetic, Microbial --- Microbial Genetic --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Microorganisms --- Crop biotechnology --- Crops --- Biotechnology --- Bioreactors --- Botany --- Plant Proteins --- Ethnopharmacology --- Genetic Phenomena --- Virus Physiological Phenomena --- Bacterial Physiological Phenomena --- Genome, Microbial --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Industrial microbiology --- Biotechnological microorganisms --- Agricultural biotechnology --- Chemistry --- biotechnologie --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant --- Biochemistry. --- Genetic Engineering. --- Genetics, Microbial. --- Molecular Biology. --- Plants. --- Recombinant Proteins. --- genetics.

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v(D)J recombination: for the community of immunologists and developmental biologists, the molecular route by which B and T lymphocytes acquire their unique function of affording adaptive immunity. Yet, for many-from experienced scientists to trainees-it represents a (rather too) sophisticated process whose true insight is excessively demanding. However, when not simplyconsidered as a private ground for a few aficionados, it can be seen as a way of understanding how maturelympho­ cytes carry on their basic functions. For the group of aficionados-which includes this editor-it is an elegant paradigm featuring many fascinating evolutionary achievements of which the biological world alone has the secret. These include a subtle biochemical principle most likelyhijacked some 470 million years ago from an ancestral gene invader and since then cleverly adapted by jawed vertebrates to precisely cleave and rearrange their antigen receptor (Ig andTCR)loci. This invader would itself have assigned the services of the nonhomologous end joining (NHEJ) DNArepair machinery as well as various DNApolymerases or transferases to work in concert with developmental clues in lymphoid cell lineages to generate an immune repertoire and efficient host surveillance while avoiding autoimmunity. Recently, important new refinements in these systems have emerged, continuing to challenge ourknowledge andbeliefs. These arejust thetopics covered by the senior authors-all established leaders in this field-and their colleagues, whilst writing the various chapters in V(D)J Recombination.

DNA-binding proteins. --- Genetic recombination. --- Genetic recombination --- DNA-binding proteins --- VDJ Recombinases --- Recombination, Genetic --- Recombinant Proteins --- DNA-Binding Proteins --- Nucleotidyltransferases --- Proteins --- Recombinases --- Genetic Processes --- Genetic Phenomena --- Amino Acids, Peptides, and Proteins --- Enzymes --- Phosphotransferases --- Enzymes and Coenzymes --- Transferases --- Chemicals and Drugs --- Phenomena and Processes --- Medical Research --- Genetics --- Medicine --- Biology --- Health & Biological Sciences --- Medicine. --- Biomedicine. --- Biomedicine general. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Chromosomes --- Recombinant DNA --- Health Workforce --- Biomedicine, general.

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This book is a practical review which focuses on computational analysis and on in silicoapproaches towards the systematic discovery of various key functional gene expression elements in microalgae as a model. So far, in this regard very little information is available. Efficient stepwise procedures for analysing the matrix attachment regions (MARs) are outlined, as well as for translation initiation sites (TIS), signal peptide (SP) sequences, gene optimization and transformation systems. These outlines can be efficiently deployed as practical models for the systematic discovery of key expression elements and for the optimization of cis/transgenes in other micro/organisms. The first chapter is an introduction on the key gene expression elements analysed in this book, including scaffold/matrix attachment regions, translation initiation sites, signal peptides as well as gene optimization. Chapter 2 focuses on systematic strategies and computational approaches toward in silico analysis of each factor. The analyses outcomes is assessed individually in chapter 3 followed by developing the specific conceptual models for each element in Chapter 4. The concluding remarks are discussed in Chapter 5. This work is of interest to computational and experimental biologists interested in transcriptional regulation analysis as well as to researchers and scientists who wish to consider the use of bioinformatics and computational biology in design, analysis, or regulatory reviews of key gene expression elements for the production of recombinant proteins experiments.

Life sciences. --- Bioinformatics. --- Proteomics. --- Systems biology. --- Computational biology. --- Life Sciences. --- Computer Appl. in Life Sciences. --- Systems Biology. --- Computational Biology/Bioinformatics. --- Recombinant proteins. --- Transgenes --- Genomics. --- Expression. --- Genome research --- Genomes --- Molecular genetics --- Expression of transgenes --- Transgene expression --- Gene expression --- Genetically engineered proteins --- Proteins --- Recombinant molecules --- Research --- Genetic engineering --- Biology --- Biological models. --- Data processing. --- Molecular biology --- Bio-informatics --- Biological informatics --- Information science --- Computational biology --- Systems biology --- Models, Biological --- Data processing --- Bioinformatics . --- Computational biology . --- Bioinformatics --- Biological systems

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Anne Kantardjieff and Weichang Zhou Mammalian Cell Cultures for Biologics Manufacturing Scott Estes and Mark Melville Mammalian Cell Line Developments in Speed and Efficiency Susan Abu-Absi, Sen Xu, Hugh Graham, Nimish Dalal, Marcus Boyer and Kedar Dave Cell Culture Process Operations for Recombinant Protein Production Sadettin S. Ozturk Equipment for Large-Scale Mammalian Cell Culture Daniel M. Marasco, Jinxin Gao, Kristi Griffiths, Christopher Froggatt, Tongtong Wang and Gan Wei Development and Characterization of a Cell Culture Manufacturing Process Using Quality by Design (QbD) Principles Michael J. Gramer Product Quality Considerations for Mammalian Cell Culture Process Development and Manufacturing Dayue Chen Safety Assurance for Biologics Manufactured in Mammalian Cell Cultures: A Multitiered Strategy Dawn M. Ecker and Thomas C. Ransohoff Mammalian Cell Culture Capacity for Biopharmaceutical Manufacturing Karthik P. Jayapal and Chetan T. Goudar Transcriptomics as a Tool for Assessing the Scalability of Mammalian Cell Perfusion Systems Enda Moran and Patrick Gammell Lifecycle Management for Recombinant Protein Production Using Mammalian Cell Culture Technology.

Chemistry. --- Pharmaceutical technology. --- Biotechnology. --- Cell culture. --- Cell Culture. --- Pharmaceutical Sciences/Technology. --- Biologicals --- Cell culture --- Vertebrates --- Proteins --- Complex Mixtures --- Culture Techniques --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Chordata --- Clinical Laboratory Techniques --- Investigative Techniques --- Animals --- Eukaryota --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Organisms --- Cell Culture Techniques --- Biological Products --- Mammals --- Recombinant Proteins --- Mechanical Engineering --- Engineering & Applied Sciences --- Bioengineering --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Mammalia --- Mammal --- Biologic Products --- Products, Biological --- Biologics --- Natural Products --- Products, Biologic --- Products, Natural --- Cell Culture --- Cell Culture Technique --- Cell Cultures --- Culture Technique, Cell --- Culture Techniques, Cell --- Eucarya --- Eukarya --- Eukaryotes --- Eukaryotas --- Eukaryote --- Animalia --- Animal --- Metazoa --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Diagnoses and Laboratory Examinations --- Diagnosis, Laboratory --- Laboratory Diagnosis --- Laboratory Examinations and Diagnoses --- Laboratory Techniques, Clinical --- Clinical Laboratory Technique --- Diagnoses, Laboratory --- Laboratory Diagnoses --- Laboratory Technique, Clinical --- Technique, Clinical Laboratory --- Techniques, Clinical Laboratory --- Chordates --- Chordate --- Culture Technique --- Technique, Culture --- Techniques, Culture --- Complex Extracts --- Crude Extracts --- Extracts, Complex --- Extracts, Crude --- Mixtures, Complex --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Vertebrate --- Pharmaceutical laboratory techniques --- Pharmaceutical laboratory technology --- Technology, Pharmaceutical --- Biologicals. --- Cultures (Biology) --- Cytology --- Technique --- Biological products --- Medical supplies --- Technology --- Chemical engineering --- Genetic engineering --- Chemistry --- Pharmaceutical technology --- Biotechnology