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This text is a guide to the basic techniques needed for molecular biology. It concentrates on the preparation, purification, and handling of nucleic acids.

Technique. --- Molecular cloning --- Recombinant DNA --- Molecular biology

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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Molecular cloning --- 575.5 --- Genetische manipulatie --- Klonen --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.

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The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.

Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Medical genetics

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Molecular genetics --- Molecular cloning --- Nucleic acids --- Biotechnology. --- Cloning, Molecular --- Genetic Techniques. --- Nucleic Acids --- Molecular cloning. --- Molecular genetics. --- Analysis --- methods. --- analysis. --- Analysis. --- DNA cloning --- Gene cloning --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Biotechnologies --- Polynucleotides --- methods --- Molecular biology --- Cloning --- Genetic engineering --- Clone cells --- Genetic Counseling --- Genetic Phenomena --- Biomolecules --- Genetic Techniques --- Biotechnology --- analysis --- Molecular Cloning --- Cosmids --- Cloning, Organism --- Acids, Nucleic --- Nucleic Acid --- Acid, Nucleic

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The ABCs of Gene Cloning, Second Edition Dominic W. S. Wong The ABCs of Gene Cloning serves as a clear and easy-to-use text that offers the reader an introductory course on the language of gene cloning. It assumes no prior knowledge on the part of the reader, while emphasizing the 'nuts and bolts' of gene cloning. The ABCs of Gene Cloning enables the reader to distinguish between a gene and a DNA, to read and write a gene sequence, to talk intelligently about gene cloning, to attend seminars without getting completely lost, and to comprehend materials in scientific publications. The ABCs of Gene Cloning contains updated information throughout, a tutorial on how to read a gene sequence, new topics on animal cloning and human genome sequencing, and rewritten chapters on human gene therapy, gene targeting, and DNA typing. Part I of this book outlines the very basic principles of biology and genetics, with emphasis only on the aspects relevant to gene cloning. The intent is to provide sufficient background materials without burdening the reader with unwarranted details. Part II describes the common techniques and approaches of gene cloning used in microbial, plant and mammalian systems. It includes topics ranging from the basic mechanics of DNA manipulation, vector construction, to genomic analysis, which is still a very new and rapidly evolving field. Part III presents selected applications of major impact on biomedicine, agriculture and related areas. Case examples are used to demonstrate how gene cloning is conducted and applied in biomedicine and agriculture. Key features: Emphasizes the vocabulary and language of gene cloning. Introduces the essentials of genetic processes relevant to cloning. Explains how to read a gene sequence. Presents basic techniques and strategies of gene cloning. Focuses on topics of applications impacting biomedicine and other fields Clear, concise, and extensively illustrated. Revised with updated information, new topics, and rewritten chapters. The ABCs of Gene Cloning is essential reading for students in biological science, biomedicine, and related disciplines, as well as others who have an interest in gaining proficiency in the language of gene cloning. The book assumes no prior knowledge of the subject, and uniquely provides the nuts and bolts for learning the art of gene cloning in a simple, logical, and thorough manner. The Author: Dominic W. S. Wong, Ph.D. is a scientist at the Western Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture. Dr. Wong is also the author of Mechanisms and Theory in Food Chemistry and Food Enzymes: Structure and Mechanism, and co-editor of Handbook of Food Enzymology.

Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Biochemistry. --- Human genetics. --- Food science. --- Plant physiology. --- Zoology. --- Biochemistry, general. --- Human Genetics. --- Food Science. --- Plant Physiology. --- Biology --- Natural history --- Animals --- Botany --- Plants --- Physiology --- Science --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Food—Biotechnology. --- Molecular cloning