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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester,
Molecular biology --- Technique --- DNA, Recombinant. --- Molecular Biology --- Protein Engineering. --- methods. --- Genetic Engineering, Protein --- Proteins, Genetic Engineering --- Genetic Engineering of Proteins --- Engineering, Protein --- Engineering, Protein Genetic --- Protein Genetic Engineering --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- Recombinant DNA --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- Molecular biochemistry --- Molecular biophysics --- Genetic Engineering --- Directed Molecular Evolution --- DNA Shuffling --- Molecular Farming --- Cloning, Molecular --- DNA Transposable Elements --- Genetic Vectors --- Genomic Library --- Gene Library --- Artificial Gene Fusion --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology
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Protein folding --- RNA --- Biomolecules --- Protein Engineering. --- Protein Folding. --- Proteins --- Protéines --- ARN --- Biomolécules --- Conformation --- Design --- analysis. --- Repliement --- Protein Folding, Globular --- Folding, Globular Protein --- Folding, Protein --- Foldings, Globular Protein --- Foldings, Protein --- Globular Protein Folding --- Globular Protein Foldings --- Protein Foldings --- Protein Foldings, Globular --- Genetic Engineering, Protein --- Proteins, Genetic Engineering --- Genetic Engineering of Proteins --- Engineering, Protein --- Engineering, Protein Genetic --- Protein Genetic Engineering --- Biological molecules --- Ribonucleic acid --- Ribose nucleic acid --- Folding of proteins --- Folding --- Protein Engineering --- Protein Folding --- analysis --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- Proteostasis --- Protein Multimerization --- Intrinsically Disordered Proteins --- Genetic Engineering --- Directed Molecular Evolution --- DNA Shuffling --- Molecular Farming --- Molecules --- Molecular biology --- Nucleic acids --- Ribose --- Protéines --- Biomolécules --- Protein folding. --- Protein --- Protein engineering. --- Technologie des protéines. --- Protein design --- Biochemical engineering --- Genetic engineering --- Conformació de proteïnes. --- Enginyeria de proteïnes. --- Revistes electròniques. --- Enginyeria de proteïnes --- Proteïnes --- Biomolècules --- Anàlisi --- Disseny --- Conformació --- Technologie des protéines. --- Enginyeria de proteïnes --- Anàlisi --- Conformació
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Molecular biology --- Molecular structure --- Cytology --- Protein folding --- RNA --- Biomolecules --- Conformation --- Design --- Biologie moléculaire --- Structure moléculaire --- Cytologie --- Molecular Structure --- Cells --- Protein Conformation --- Protein Engineering --- Protein Folding --- ultrastructure --- Molecular Structure. --- Protein Conformation. --- Protein Engineering. --- Protein Folding. --- ultrastructure. --- Protein Folding, Globular --- Folding, Globular Protein --- Folding, Protein --- Foldings, Globular Protein --- Foldings, Protein --- Globular Protein Folding --- Globular Protein Foldings --- Protein Foldings --- Protein Foldings, Globular --- Genetic Engineering, Protein --- Proteins, Genetic Engineering --- Genetic Engineering of Proteins --- Engineering, Protein --- Engineering, Protein Genetic --- Protein Genetic Engineering --- Conformation, Protein --- Conformations, Protein --- Protein Conformations --- Structure, Molecular --- Molecular Structures --- Structures, Molecular --- Biological molecules --- Ribonucleic acid --- Ribose nucleic acid --- Folding of proteins --- Proteins --- Folding --- Cytology. --- Molecular biology. --- Molecular structure. --- Protein folding. --- Proteostasis --- Protein Multimerization --- Intrinsically Disordered Proteins --- Genetic Engineering --- Directed Molecular Evolution --- DNA Shuffling --- Molecular Farming --- Models, Molecular --- Chemical structure --- Structural bioinformatics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Molecules --- Nucleic acids --- Ribose --- Biologie moléculaire --- Structure moléculaire --- Cell --- Cell Biology --- Protein engineering. --- Protéines --- ARN --- Structure moléculaire. --- Technologie des protéines. --- molecular structure. --- Conformation. --- Repliement --- Protein design --- Biochemical engineering --- Genetic engineering --- Protein conformation --- Enginyeria de proteïnes --- Biologia molecular --- Estructura molecular --- Citologia
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