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This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.

Polymerase chain reaction.

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PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery

Gene amplification. --- Genomes. --- Genomics. --- Polymerase chain reaction. --- Polymerase chain reaction --- Gene amplification --- Genomics

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This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.Key Features* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies* Includes overviews of* Optical probes and reagents

Polymerase chain reaction. --- Cell physiology. --- Ions --- Physiological effect. --- Analysis.

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Polymerase chain reaction. --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases

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This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.

Polymerase chain reaction. --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Biochemistry