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Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.
Confocal microscopy. --- Fluorescence microscopy. --- Image processing -- Digital techniques. --- Microbiology -- Technique. --- Microscopy, Confocal. --- Microscopy, Electron, Scanning. --- Microscopy. --- Confocal microscopy --- Biology --- Health & Biological Sciences --- Microscopy --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Life sciences. --- Biotechnology. --- Cell biology. --- Life Sciences. --- Biological Microscopy. --- Cell Biology. --- Optics --- Cytology. --- Chemical engineering --- Genetic engineering --- Cell biology --- Cellular biology --- Cells --- Cytologists
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This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.
Biological microscopy --- Confocal microscopy. --- Microscopie confocale --- Confocal microscopy --- Microscopy --- Diagnostic Imaging --- Image Processing, Computer-Assisted --- Diagnostic Techniques and Procedures --- Computing Methodologies --- Investigative Techniques --- Imaging, Three-Dimensional --- Microscopy, Confocal --- Information Science --- Diagnosis --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemistry --- Biology --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Biology - General --- Biochemistry --- EPUB-LIV-FT LIVBIOLO LIVBIOMO LIVMEDEC SPRINGER-B --- Life sciences. --- Biotechnology. --- Biochemistry. --- Life Sciences. --- Biochemistry, general. --- Chemical engineering --- Genetic engineering --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Medical sciences --- Composition
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In recent years the relevance of non-invasive bioimaging techniques in the field of melanoma screening has steadily increased. In the new era of “clinicoimaging” diagnosis, reflectance confocal microscopy (RCM) will have a major impact on the diagnosis and management of neoplastic and inflammatory skin diseases. This book focuses on the use and significance of in vivo RCM for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated with numerous confocal images and structured in a reader-friendly way. The contents include: • detailed information on the most relevant and up-to-date aspects of RCM, • schematic drawings summarizing and explaining the most important RCM criteria, and • a chapter specifically devoted to bridging the gap between dermoscopy, RCM, and histopathology. At the end of each chapter, core messages recapitulate the most pertinent aspects. Reflectance Confocal Microscopy for Skin Diseases will be a valuable resource for all physicians involved in the diagnosis and treatment of neoplastic and inflammatory skin diseases.
Laser Therapy -- methods. --- Skin -- Laser surgery. --- Skin Diseases -- surgery. --- Skin and Connective Tissue Diseases --- Microscopy --- Investigative Techniques --- Diagnostic Imaging --- Diseases --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Diagnostic Techniques and Procedures --- Diagnosis --- Microscopy, Confocal --- Skin Diseases --- Medicine --- Health & Biological Sciences --- Dermatology --- Skin --- Microscopy. --- Spectrum analysis. --- Diagnosis. --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectroscopy --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Medicine. --- Dermatology. --- Oncology. --- Otorhinolaryngology. --- Pediatrics. --- Plastic surgery. --- Medicine & Public Health. --- Plastic Surgery. --- Chemistry, Analytic --- Interferometry --- Optics --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Light --- Spectroscope --- Qualitative --- Oncology . --- Surgery. --- Paediatrics --- Pediatric medicine --- Children --- Ear, nose, and throat diseases --- ENT diseases --- Otorhinolaryngology --- Surgery, Primitive --- Tumors --- Health and hygiene --- Aesthetic surgery --- Cosmetic surgery --- Plastic surgery --- Reconstructive surgery --- Surgery, Aesthetic --- Surgery, Cosmetic --- Surgery, Reconstructive --- Transplantation of organs, tissues, etc. --- Plastic surgeons
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This atlas will illustrate the distribution and morphological features of interstitial cells of Cajal (ICC) which are the key cells to understanding of the regulatory mechanism of gastrointestinal motility, since ICC act as both pacemaker and as intermediates in neural transmission, and since ICC show specific distribution patterns depending on their anatomical positions. All subtypes of ICC located in the different tissue layers and different levels of the gastrointestinal tract will be revealed by immunohistochemistry for Kit receptors and nerves by using mainly whole-mount stretch preparation of the guinea-pig tissues. Three-dimensional reconstruction of confocal images will particularly help the readers to understand the peculiar arrangement of ICC networks in situ and the correlation between ICC and nerves. Electron micrographs will help illustrate the characteristic features of ICC and their ultrastructural differences from fibroblasts, smooth muscles and other interstitial cells.
Biomedicine. --- Gastrointestinal system -- Cytology -- Atlases. --- Medicine. --- Histocytochemistry --- Muscle Cells --- Microscopy --- Immunologic Tests --- Immunochemistry --- Biochemistry --- Laboratory Techniques and Procedures --- Diagnostic Imaging --- Cytological Techniques --- Investigative Techniques --- Histological Techniques --- Immunologic Techniques --- Cells --- Histology --- Clinical Laboratory Techniques --- Diagnosis --- Anatomy --- Diagnostic Techniques and Procedures --- Biological Science Disciplines --- Chemistry --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Natural Science Disciplines --- Disciplines and Occupations --- Immunohistochemistry --- Interstitial Cells of Cajal --- Microscopy, Confocal --- Biology --- Medicine --- Health & Biological Sciences --- Cytology --- Urology & Nephrology --- Interstitial cystitis. --- Gastrointestinal system --- Diseases. --- Bladder pain syndrome --- BPS (Bladder pain syndrome) --- Hunner's ulcer --- IC (Interstitial cystitis) --- IC/PBS (Interstitial cystitis) --- Painful bladder syndrome --- PBS (Painful bladder syndrome) --- Biomedicine general. --- Cystitis --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Health Workforce --- Biomedicine, general.
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Microscopy, Confocal --- Microscopy, Fluorescence --- Fluorescence Recovery After Photobleaching --- Luminescent Proteins --- Spectrometry, Fluorescence --- Microscopy --- Microscopie --- methods --- analysis --- Technique --- Analytical biochemistry --- Biochemistry --- Biotechnology --- Technique. --- Chemistry. --- Analytical biochemistry. --- Biotechnology. --- Biochemistry. --- Microscopy. --- Biological Microscopy. --- Analytical Chemistry. --- Biochemistry, general. --- Methods --- Spectrum Analysis --- Investigative Techniques --- Fluorometry --- Proteins --- Luminescent Measurements --- Diagnostic Imaging --- Chemistry Techniques, Analytical --- Amino Acids, Peptides, and Proteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Photometry --- Chemicals and Drugs --- Diagnostic Techniques and Procedures --- Diagnosis --- Bioengineering --- Biology --- Mechanical Engineering --- Engineering & Applied Sciences --- Health & Biological Sciences --- Antemortem Diagnosis --- Diagnoses and Examinations --- Examinations and Diagnoses --- Postmortem Diagnosis --- Antemortem Diagnoses --- Diagnoses --- Diagnoses, Antemortem --- Diagnoses, Postmortem --- Diagnosis, Antemortem --- Diagnosis, Postmortem --- Postmortem Diagnoses --- Disease --- Diagnostic Technics and Procedures --- Technics and Procedures, Diagnostic --- Techniques and Procedures, Diagnostic --- Photometries --- Analytical Chemistry Methods --- Analytical Chemistry Techniques --- Analytical Chemistry Method --- Analytical Chemistry Technique --- Chemistry Method, Analytical --- Chemistry Methods, Analytical --- Chemistry Technique, Analytical --- Method, Analytical Chemistry --- Methods, Analytical Chemistry --- Technique, Analytical Chemistry --- Techniques, Analytical Chemistry --- Imaging, Diagnostic --- Imaging, Medical --- Medical Imaging --- Bioluminescence Measurements --- Bioluminescent Assays --- Bioluminescent Measurements --- Chemiluminescence Measurements --- Chemiluminescent Assays --- Chemiluminescent Measurements --- Chemoluminescence Measurements --- Luminescence Measurements --- Luminescent Assays --- Luminescent Techniques --- Phosphorescence Measurements --- Phosphorescent Assays --- Phosphorescent Measurements --- Assay, Bioluminescent --- Assay, Chemiluminescent --- Assay, Luminescent --- Assay, Phosphorescent --- Assays, Bioluminescent --- Assays, Chemiluminescent --- Assays, Luminescent --- Assays, Phosphorescent --- Bioluminescence Measurement --- Bioluminescent Assay --- Bioluminescent Measurement --- Chemiluminescence Measurement --- Chemiluminescent Assay --- Chemiluminescent Measurement --- Chemoluminescence Measurement --- Luminescence Measurement --- Luminescent Assay --- Luminescent Measurement --- Luminescent Technique --- Measurement, Bioluminescence --- Measurement, Bioluminescent --- Measurement, Chemiluminescence --- Measurement, Chemiluminescent --- Measurement, Chemoluminescence --- Measurement, Luminescence --- Measurement, Luminescent --- Measurement, Phosphorescence --- Measurement, Phosphorescent --- Measurements, Bioluminescence --- Measurements, Bioluminescent --- Measurements, Chemiluminescence --- Measurements, Chemiluminescent --- Measurements, Chemoluminescence --- Measurements, Luminescence --- Measurements, Luminescent --- Measurements, Phosphorescence --- Measurements, Phosphorescent --- Phosphorescence Measurement --- Phosphorescent Assay --- Phosphorescent Measurement --- Technique, Luminescent --- Techniques, Luminescent --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Fluorometric Analysis --- Fluorimetry --- Analysis, Fluorometric --- Investigative Technics --- Investigative Technic --- Investigative Technique --- Technic, Investigative --- Technics, Investigative --- Technique, Investigative --- Techniques, Investigative --- Microscopies --- Analysis, Spectrum --- Spectrometry --- Spectroscopy --- Fluorescence Microscopy --- Immunofluorescence Microscopy --- Microscopy, Immunofluorescence --- Fluorescence Microscopies --- Immunofluorescence Microscopies --- Microscopies, Fluorescence --- Microscopies, Immunofluorescence --- Methodological Studies --- Methodological Study --- Procedures --- Studies, Methodological --- Study, Methodological --- Method --- Procedure --- Bioluminescent Proteins --- Photoproteins --- Proteins, Bioluminescent --- Proteins, Luminescent --- Fluorescence Spectrophotometry --- Fluorescence Spectroscopy --- Spectrofluorometry --- Fluorescence Spectrometry --- Spectrophotometry, Fluorescence --- Spectroscopy, Fluorescence --- Confocal Laser Scanning Microscopy --- Confocal Microscopy --- Confocal Microscopy, Scanning Laser --- Laser Microscopy --- Laser Scanning Confocal Microscopy --- Laser Scanning Microscopy --- Microscopy, Confocal, Laser Scanning --- Confocal Microscopies --- Laser Microscopies --- Laser Scanning Microscopies --- Microscopies, Confocal --- Microscopies, Laser --- Microscopies, Laser Scanning --- Microscopy, Laser --- Microscopy, Laser Scanning --- Scanning Microscopies, Laser --- Scanning Microscopy, Laser --- Microscopical technique --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Analytic biochemistry --- diagnosis --- Composition --- FRAP (Fluorescence Recovery After Photobleaching) --- Fluorescence Photobleaching Recovery --- FRAPs (Fluorescence Recovery After Photobleaching) --- Analytical chemistry. --- Life sciences. --- Life Sciences, general. --- Optics --- Chemistry --- Medical sciences --- Chemical engineering --- Genetic engineering --- Physical sciences --- Biosciences --- Sciences, Life --- Science --- Analysis, Chemical --- Analytical chemistry --- Chemical analysis --- Metallurgical analysis --- Mineralogy, Determinative --- Chemistry, Analytic --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytic chemistry --- Microscopy - Technique.
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