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Laboratory techniques in biochemistry and molecular biology
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ISBN: 0444502025 1281715859 9786611715854 0080858961 0444502017 9780444502018 Year: 1999 Publisher: Amsterdam Oxford North-Holland Pub. Co.

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Abstract

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as


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Antibody drug discovery
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ISBN: 184816629X 9781848166295 1848166281 9781848166288 Year: 2012 Publisher: London Imperial College Press

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Monoclonal antibodies have become important treatments for cancer, inflammation and a wide range of other diseases, representing an increasing share of the most successful pharmaceutical markets. The technologies to discover these drugs have been developed by select centers of excellence in industry and academia, and are continually being fine tuned in the race to identify the best antibody-based drug candidates and accelerate their paths to patients. The objective of this volume is to provide a series of guides to those evaluating and preparing to enter particular areas within the field and t


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Enzyme-linked Immunosorbent Assay (ELISA) : From A to Z
Authors: --- --- ---
ISBN: 981106766X 9811067651 Year: 2018 Publisher: Singapore : Springer Singapore : Imprint: Springer,

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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .

Practice and theory of enzyme immunoassays
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ISBN: 9780444806345 0444806342 9786611759636 1281759635 0080858848 9780080858845 9781281759634 0444806334 9780444806338 Year: 1985 Volume: 15 Publisher: Amsterdam Elsevier

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Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.

Keywords

Immunoenzyme technique. --- Science. --- Chemistry. --- Natural history. --- Immunoenzyme Techniques --- Immunoenzyme Techniques. --- 577.15.083.3 --- Antibody Enzyme Technic, Unlabeled --- Enzyme-Labeled Antibody Technic --- Immunoenzyme Technics --- Immunoperoxidase Technics --- Peroxidase-Antiperoxidase Complex Technic --- Peroxidase-Labeled Antibody Technic --- Antibody Enzyme Technique, Unlabeled --- Enzyme Immunoassay --- Enzyme-Labeled Antibody Technique --- Immunoassay, Enzyme --- Immunoperoxidase Techniques --- Peroxidase-Antiperoxidase Complex Technique --- Peroxidase-Labeled Antibody Technique --- Antibody Technic, Enzyme-Labeled --- Antibody Technic, Peroxidase-Labeled --- Antibody Technics, Enzyme-Labeled --- Antibody Technics, Peroxidase-Labeled --- Antibody Technique, Enzyme-Labeled --- Antibody Technique, Peroxidase-Labeled --- Antibody Techniques, Enzyme-Labeled --- Antibody Techniques, Peroxidase-Labeled --- Enzyme Immunoassays --- Enzyme Labeled Antibody Technic --- Enzyme Labeled Antibody Technique --- Enzyme-Labeled Antibody Technics --- Enzyme-Labeled Antibody Techniques --- Immunoassays, Enzyme --- Immunoenzyme Technic --- Immunoenzyme Technique --- Immunoperoxidase Technic --- Immunoperoxidase Technique --- Peroxidase Antiperoxidase Complex Technic --- Peroxidase Antiperoxidase Complex Technique --- Peroxidase Labeled Antibody Technic --- Peroxidase Labeled Antibody Technique --- Peroxidase-Antiperoxidase Complex Technics --- Peroxidase-Antiperoxidase Complex Techniques --- Peroxidase-Labeled Antibody Technics --- Peroxidase-Labeled Antibody Techniques --- Technic, Enzyme-Labeled Antibody --- Technic, Immunoenzyme --- Technic, Immunoperoxidase --- Technic, Peroxidase-Antiperoxidase Complex --- Technic, Peroxidase-Labeled Antibody --- Technics, Enzyme-Labeled Antibody --- Technics, Immunoenzyme --- Technics, Immunoperoxidase --- Technics, Peroxidase-Antiperoxidase Complex --- Technics, Peroxidase-Labeled Antibody --- Technique, Enzyme-Labeled Antibody --- Technique, Immunoenzyme --- Technique, Immunoperoxidase --- Technique, Peroxidase-Antiperoxidase Complex --- Technique, Peroxidase-Labeled Antibody --- Techniques, Enzyme-Labeled Antibody --- Techniques, Immunoenzyme --- Techniques, Immunoperoxidase --- Techniques, Peroxidase-Antiperoxidase Complex --- Techniques, Peroxidase-Labeled Antibody --- Enzymes. Catalysts of biological reactions. Enzymology--?.083.3 --- 577.15.083.3 Enzymes. Catalysts of biological reactions. Enzymology--?.083.3 --- Cytochemistry. --- Cell chemistry --- Biochemistry --- Cytology --- Antibody enzyme techniques, Unlabeled --- Immunoperoxidase technique --- Peroxidase labeled antibody technique --- Enzymatic analysis --- Immunoassay --- Immunology --- Protein binding --- Technique --- Basic Sciences. Chemistry --- Biochemistry. --- Enzymimmunassay. --- Enzym immunoassay. --- Immunoenzyme Techniques - laboratory manuals --- Enzymes --- Immunochemical analysis

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