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The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Medical genetics
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Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Clonatge molecular
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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Molecular cloning --- 575.5 --- Genetische manipulatie --- Klonen --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.
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Hybridomas --- Monoclonal antibodies --- Antibodies, Monoclonal --- Hybridomas. --- Monoclonal antibodies. --- Monoclonal immunoglobulins --- Myeloma-spleen cell hybrids --- Biotechnology. --- Immunology. --- Medical Technology. --- Immunoglobulins --- Molecular cloning --- Clone cells --- Lymphocytes --- Plasmacytoma --- Somatic hybrids --- Agriculture Sciences --- Engineering --- Soil Chemistry, Microbiology, Fertility & Fertilizers --- Biotechnology
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Georges Köhler (1946-1995) was one of the most prominent German scientists of recent history. In 1984, at an age of 38, he received the Nobel Prize in Physiology or Medicine, together with N.K. Jerne and C. Milstein, for inventing the technique for generating monoclonal antibodies. This method and its subsequent applications had an enormous impact on basic research, medicine and the biotech industry. In the same year, Köhler became one of the directors of the Max-Planck-Institute of Immunobiology in Freiburg; his unfortunate premature death in 1995 set an end to his extraordinary career. Prof. Klaus Eichmann, who had joined the institute as director in 1981, and had invited Köhler to become his codirector, is one of the people who were closest to Georges Köhler. This scientific biography commemorates the 10th anniversary of Köhler's untimely death. It describes his scientific and personal biography, based on 10 years of close personal and professional relationship between Eichmann and Köhler, as well as interviews with many colleagues and friends, including his "Doktorvater" and mentor Fritz Melchers. Köhler's scientific achievements are explained in a way to make them understandable for the general public and discussed in the historical context of the immunological research at the time.
Immunologists --- Hybridomas. --- Monoclonal antibodies. --- Köhler, Georges. --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Myeloma-spleen cell hybrids --- Clone cells --- Lymphocytes --- Monoclonal antibodies --- Plasmacytoma --- Somatic hybrids --- Immunology --- Medical scientists --- Immunology. --- Medicine. --- Biomedicine general. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Immunobiology --- Serology --- Health Workforce --- Biomedicine, general. --- Köhler, Georges --- Kohler, Georges.
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The ABCs of Gene Cloning, Second Edition Dominic W. S. Wong The ABCs of Gene Cloning serves as a clear and easy-to-use text that offers the reader an introductory course on the language of gene cloning. It assumes no prior knowledge on the part of the reader, while emphasizing the 'nuts and bolts' of gene cloning. The ABCs of Gene Cloning enables the reader to distinguish between a gene and a DNA, to read and write a gene sequence, to talk intelligently about gene cloning, to attend seminars without getting completely lost, and to comprehend materials in scientific publications. The ABCs of Gene Cloning contains updated information throughout, a tutorial on how to read a gene sequence, new topics on animal cloning and human genome sequencing, and rewritten chapters on human gene therapy, gene targeting, and DNA typing. Part I of this book outlines the very basic principles of biology and genetics, with emphasis only on the aspects relevant to gene cloning. The intent is to provide sufficient background materials without burdening the reader with unwarranted details. Part II describes the common techniques and approaches of gene cloning used in microbial, plant and mammalian systems. It includes topics ranging from the basic mechanics of DNA manipulation, vector construction, to genomic analysis, which is still a very new and rapidly evolving field. Part III presents selected applications of major impact on biomedicine, agriculture and related areas. Case examples are used to demonstrate how gene cloning is conducted and applied in biomedicine and agriculture. Key features: Emphasizes the vocabulary and language of gene cloning. Introduces the essentials of genetic processes relevant to cloning. Explains how to read a gene sequence. Presents basic techniques and strategies of gene cloning. Focuses on topics of applications impacting biomedicine and other fields Clear, concise, and extensively illustrated. Revised with updated information, new topics, and rewritten chapters. The ABCs of Gene Cloning is essential reading for students in biological science, biomedicine, and related disciplines, as well as others who have an interest in gaining proficiency in the language of gene cloning. The book assumes no prior knowledge of the subject, and uniquely provides the nuts and bolts for learning the art of gene cloning in a simple, logical, and thorough manner. The Author: Dominic W. S. Wong, Ph.D. is a scientist at the Western Regional Research Center, Agricultural Research Service, U. S. Department of Agriculture. Dr. Wong is also the author of Mechanisms and Theory in Food Chemistry and Food Enzymes: Structure and Mechanism, and co-editor of Handbook of Food Enzymology.
Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Biochemistry. --- Human genetics. --- Food science. --- Plant physiology. --- Zoology. --- Biochemistry, general. --- Human Genetics. --- Food Science. --- Plant Physiology. --- Biology --- Natural history --- Animals --- Botany --- Plants --- Physiology --- Science --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Food—Biotechnology. --- Molecular cloning
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Genetic engineering --- Molecular genetics --- Molecular cloning --- Nucleic acids --- Génie génétique --- Génétique moléculaire --- Clonage moléculaire --- Acides nucléiques --- Genetic engineering. --- Molecular cloning. --- Molecular genetics. --- Analysis --- Analyse --- Analysis. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Polynucleotides --- Genetics --- Molecular biology --- Cloning --- Clone cells --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Biomolecules
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Gene Probes
Biological techniques --- Neuropathology --- Molecular biology --- Neurobiology --- DNA probes --- Gene expression --- Neurobiologie --- Biologie moléculaire --- Sondes ADN --- Expression génique --- Technique --- Biologie moléculaire --- Expression génique --- Mammals --- Gene expression. --- Hybridization. --- Molecular cloning. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Hybridisation --- Hybridism --- Breeding --- Heredity --- Mendel's law --- Genes --- Genetic regulation --- Nervous system. --- Expression
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MEDICAL --- Immunology --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Monoclonal antibodies --- Hybridomas. --- Mice as laboratory animals. --- Synthesis --- Methodology. --- Myeloma-spleen cell hybrids --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Laboratory animals --- Clone cells --- Lymphocytes --- Plasmacytoma --- Somatic hybrids --- Immunoglobulins --- Molecular cloning --- Therapeutic use. --- Hybridomas --- Mice as laboratory animals --- Methodology
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Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. Part III & IV present application technologies of major impact in agriculture, biomedicine, and related areas. The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning.
Medicine. --- Human genetics. --- Food --- Plant physiology. --- Zoology. --- Biomedicine. --- Human Genetics. --- Food Science. --- Plant Physiology. --- Biotechnology. --- Molecular cloning --- Methodology. --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Food science. --- Biology --- Natural history --- Animals --- Botany --- Plants --- Physiology --- Science --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Food—Biotechnology.
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