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Book
Recombinant protein expression in microbial systems
Authors: --- ---
Year: 2014 Publisher: Frontiers Media SA

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Abstract

With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.


Book
Dehors ! : le graffiti a Bruxelles
Authors: --- ---
ISBN: 9782930018874 2930018879 Year: 2011 Volume: *3 Publisher: Bruxelles : CFC Musée d'Ixelles,

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Quelle est l'histoire du graffiti à Bruxelles ? Quelles sont ses origines, ses règles et ses pratiques ? Qui sont ses artistes de l'ombre ? Dehors ! Le graffiti à Bruxelles tente de répondre à ces questions et met à l'épreuve le double problème de la place du graffiti dans l'art et dans la ville, en interrogeant son rôle dans l'enrichissement artistique de la capitale de l'Europe. Adrien Grimmeau a analysé tous les parcours, de la rue aux galeries, en s'appuyant sur une documentation rare et des témoignages originaux, riches et colorés, dans l'esprit même des nouveaux codes de lecture nés de cette esthétique irrégulière. Pour la première fois, des graffeurs des générations successives ont ouvert leurs archives et partagé leur passion commune pour l'art urbain. Ce premier ouvrage de référence sur le sujet fait passer « l'art de la rue » de l'éphémère à l'indélébile. L'exposition inspirée du livre "L'art du graffiti à Bruxelles" se tient au Musée d'Ixelles (rue J. Van Volsem 71, 1050 Bruxelles) du 16 juin au 4 septembre 2011.


Book
Shadow exchanges along the New Silk Roads
Authors: ---
ISBN: 9789048541348 9048541344 9789462988934 9462988935 Year: 2020 Publisher: Amsterdam : Amsterdam University Press,

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Long before China promulgated the official One Belt One Road initiatives, vast networks of cross-border exchanges already existed across Asia and Eurasia. The dynamics of such trade and resource flows have largely been outside state control, and are pushed to the realm of the shadow economy. The official initiative is a state-driven attempt to enhance the orderly flow of resources across countries along the Belt and Road, hence extending the reach of the states to the shadow economies. This volume offers a bottom-up view of the transborder informal exchanges across Asia and Eurasia, and analyses its clash and mesh with the state-orchestrated Belt and Road cooperation. By undertaking a comparative study of country cases along the new silk roads, the book underlines the intended and unintended consequences of such competing routes of connectivity on the socio-economic conditions of local communities.


Book
Banksy : you are an acceptable level of threat and if you were not you would know about it.
Authors: --- ---
ISBN: 9781908211088 Year: 2012 Publisher: Darlington Carpet bombing culture


Periodical
Animal biotelemetry.
ISSN: 20503385 Year: 2013 Publisher: London : Biomed Central,

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"Animal Biotelemetry is an open access peer-reviewed journal that publishes the results of studies utilizing telemetric techniques (including biologgers) to understand physiological, behavioural, and ecological mechanisms in a broad range of environments (e.g. terrestrial, freshwater and marine) and taxa. The journal also welcomes descriptions and validations of newly developed tagging techniques and tracking technologies, as well as methods for analyzing telemetric data."


Book
Current Advances and Challenges in Fisheries and Aquaculture Science: Feature Papers for the New Journey of Fishes
Authors: --- ---
Year: 2022 Publisher: Basel MDPI - Multidisciplinary Digital Publishing Institute

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This addresses current advances and challenges in fisheries and aquaculture science. Exposure of larval fish to elevated temperatures during embryological development may induce craniofacial and morphological alterations, which are suggested possible impacts of global warming. Molecular markers shed new light on the ontogenetic migration of stream fishes. Fast growth rates, early age at maturity, moderate fecundity, and diverse diet explain the potential for introduced fishes to dominate fish communities in their native and introduced range. Taking videos of marine benthic habitats supports low-impact, real-time monitoring of species occurrence. Among heavily fished species, almost half had outdated demographic assessments that would benefit from the integration of data from fisheries sources and improved collaboration among fishery stakeholders and managers. The continued growth of aquaculture will depend upon developing feeds that improve the growth, oxidative status, and immune response of fed cultured organisms. New aquaculture feedstuffs might be derived from plants or microbes, and new additives would include ghrelins and dietary symbiotics. The effects of these constituents on survival, growth, gut histomorphology, immune response were assessed for cultured freshwater and marine species. The results provide suggestions for advances in aquafeeds for the species studied and for cultured fishes more generally. The scientific advances realized with the use of new tools provide the basis for addressing global challenges to fisheries, aquaculture and for ongoing scientific research.


Book
In het teken van de kunst : x manieren van tekenen in de hedendaagse kunst
Authors: --- --- --- ---
ISBN: 9038201192 Year: 1997 Publisher: Gent Academia Press Universiteit Gent


Book
Racial Reconciliation and Privilege : The Debate Within the Seventh-Day Adventist Church on Regional Conferences
Author:
ISBN: 1946230383 1946230367 Year: 2017 Publisher: Claremont, California : Claremont Press,


Book
Women in New Religions
Author:
ISBN: 1479841498 1479822493 9781479822492 9781479847990 1479847992 9781479816026 1479816027 9781479841493 Year: 2015 Publisher: New York : Baltimore, Md. : New York Univ. Press, Project MUSE,

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Women in New Religions offers an engaging look at women’s evolving place in the birth and development of new religious movements. It focuses on four disparate new religions—Mormonism, Seventh-day Adventism, The Family International, and Wicca—to illuminate their implications for gender socialization, religious leadership and participation, sexuality, and family ideals. Religious worldviews and gender roles interact with one another in complicated ways. This is especially true within new religions, which frequently set roles for women in ways that help the movements to define their boundaries in relation to the wider society. As new religious movements emerge, they often position themselves in opposition to dominant society and concomitantly assert alternative roles for women. But these religions are not monolithic: rather than defining gender in rigid and repressive terms, new religions sometimes offer possibilities to women that are not otherwise available. Vance traces expectations for women as the religions emerge, and transformation of possibilities and responsibilities for women as they mature. Weaving theory with examination of each movement’s origins, history, and beliefs and practices, this text contextualizes and situates ideals for women in new religions. The book offers an accessible analysis of the complex factors that influence gender ideology and its evolution in new religious movements, including the movements’ origins, charismatic leadership and routinization, theology and doctrine, and socio-historical contexts. It shows how religions shape definitions of women’s place in a way that is informed by response to social context, group boundaries, and identity. Additional Resources


Book
Plant Proteomic Research 2.0
Author:
ISBN: 3039210637 3039210629 Year: 2019 Publisher: MDPI - Multidisciplinary Digital Publishing Institute

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Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research. Proteomics offers one of the best options for the functional analysis of translated regions of the genome, generating a wealth of detailed information regarding the intrinsic mechanisms of plant stress responses. Various proteomic approaches are being exploited extensively for elucidating master regulator proteins which play key roles in stress perception and signaling, and these approaches largely involve gel-based and gel-free techniques, including both label-based and label-free protein quantification. Furthermore, post-translational modifications, subcellular localization, and protein–protein interactions provide deeper insight into protein molecular function. Their diverse applications contribute to the revelation of new insights into plant molecular responses to various biotic and abiotic stressors.

Keywords

14-3-3 proteins --- n/a --- targeted two-dimensional electrophoresis --- somatic embryogenesis --- nitrogen metabolism --- subtilase --- Sporisorium scitamineum --- non-orthodox seed --- antioxidant activity --- sweet potato plants infected by SPFMV --- photosynthesis --- B. acuminata petals --- chlorophyll deficiency --- seed proteomics --- imbibition --- pollination --- Sarpo Mira --- qRT-PCR --- holm oak --- tuber phosphoproteome --- isobaric tags for relative and absolute quantitation (iTRAQ) --- Quercus ilex --- nucleotide pyrophosphatase/phosphodiesterase --- lettuce --- ?-subunit --- protein phosphatase --- germination --- drought stress --- pyruvate biosynthesis --- weakening of carbon metabolism --- differential proteins --- heterotrimeric G protein --- organ --- LC-MS-based proteomics --- potato proteomics --- smut --- gel-free/label-free proteomics --- ? subunit --- shotgun proteomics --- 2D --- chloroplast --- proteome functional annotation --- Phalaenopsis --- Clematis terniflora DC. --- wheat --- Dn1-1 --- carbon metabolism --- physiological responses --- Zea mays --- phenylpropanoid biosynthesis --- ISR --- mass spectrometric analysis --- patatin --- leaf --- pea (Pisum sativum L.) --- maize --- ergosterol --- Camellia sinensis --- seed storage proteins --- silver nanoparticles --- elevated CO2 --- metacaspase --- SPV2 and SPVG --- SnRK1 --- MALDI-TOF/TOF --- (phospho)-proteomics --- leaf spot --- rice isogenic line --- wheat leaf rust --- pathway analysis --- phosphoproteome --- sugarcane --- senescence --- Oryza sativa L. --- Arabidopsis thaliana --- heat stress --- gene ontology --- innate immunity --- Pseudomonas syringae --- bolting --- chlorophylls --- shoot --- Simmondsia chinensis --- RT-qPCR --- stresses responses --- Solanum tuberosum --- seeds --- GC-TOF-MS --- sucrose --- proteome --- Puccinia recondita --- cultivar --- Zea mays L. --- secondary metabolism --- ROS --- Ricinus communis L. --- after-ripening --- cadmium --- Stagonospora nodorum --- virus induced gene silencing --- quantitative proteomics --- sweet potato plants non-infected by SPFMV --- affinity chromatography --- population variability --- GS3 --- fungal perception --- ammonium --- transcriptome profiling --- mass spectrometry analysis --- papain-like cysteine protease (PLCP) --- cold stress --- nitrate --- late blight disease --- early and late disease stages --- seed imbibition --- lesion mimic mutant --- protease --- proteome map --- seed dormancy --- petal --- 2-DE proteomics --- 2D DIGE --- root --- Phytophthora infestans --- differentially abundant proteins (DAPs) --- polyphenol oxidase --- degradome --- flavonoid --- 14-3-3 --- caspase-like --- proteomics --- RGG4 --- co-infection --- plasma membrane --- chlorotic mutation --- Medicago sativa --- RGG3 --- glycolysis --- barley --- 2-DE --- protein phosphorylation --- western blotting --- N utilization efficiency --- rice --- plant pathogenesis responses --- high temperature --- data-independent acquisition --- pattern recognition receptors --- vegetative storage proteins --- leaf cell wall proteome --- plant-derived smoke --- iTRAQ --- starch --- proteome profiling --- Morus

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