Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Techniques for separating cells are needed in many areas of cell biology. This text presents modern methods from the laboratories of experts in the field and includes tested, reproducible protocols, hints and tips and troubleshooting techniques.

Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Separation

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.

Regenerative medicine. --- Stem cells. --- Regenerative Medicine and Tissue Engineering. --- Stem Cell Biology. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Medicine --- Regeneration (Biology) --- immune response --- cell sorting --- stem cells --- cell isolation --- apoptosis --- Open Access

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

Flow cytometry. --- Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Separation --- Cell separation --- Cytodiagnosis --- Flow cytometry --- Cell Separation --- Flow Cytometry --- Cytologic diagnosis --- Diagnosis, Cytologic --- Diagnostic cytopathology --- Diagnosis, Laboratory --- Pathology, Cellular --- methods

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Bionanoparticles such as microorganisms and exosomes are recognized as important targets for clinical applications, food safety, and environmental monitoring. Other nanoscale biological particles, including liposomes, micelles, and functionalized polymeric particles are widely used in nanomedicines. The recent development of microfluidic and nanofluidic technologies has enabled the separation and analysis of these species in a lab-on-a-chip platform, while there are still many challenges to address before these analytical tools can be adopted in practice. For example, the complex matrices within which these species reside in create a high background for their detection. Their small dimension and often low concentration demand creative strategies to amplify the sensing signal and enhance the detection speed. This Special Issue aims to recruit recent discoveries and developments of micro- and nanofluidic strategies for the processing and analysis of biological nanoparticles. The collection of papers will hopefully bring out more innovative ideas and fundamental insights to overcome the hurdles faced in the separation and detection of bionanoparticles.

n/a --- magnetic field --- microfluidic device --- ballpoint pen printing --- paper-based microfluidic device --- online analysis --- nanoporous membrane --- dielectric film --- digital microfluidic chip --- HIV diagnostics --- precipitation --- optically induced dielectrophoresis (ODEP) --- digital microfluidic device --- fluorescence --- ferrofluids --- cancer metastasis --- flow focusing --- image processing --- electrowetting --- light diffraction --- lensfree --- nanoparticle characterization --- multi-step assay --- cell isolation --- biomarker detection --- microparticles --- conductive electrode --- single particle analysis --- plastic wrap --- second-hand smoke --- flow control --- surface acoustic wave --- droplet actuation --- circulating tumour cells (CTCs) --- lipid nanoparticles --- crop disease --- cross-flow filtration --- oxidized hollow mesoporous carbon nanosphere --- microfluidic systems --- 3-ethenylpyridine --- microfluidic --- microfluidics --- COMSOL --- plug flow mixer