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Transgenes --- Muscle, Skeletal --- Genetic Heterogeneity --- Genetic Therapy --- Receptors, Cell Surface --- Gene Transfer Techniques --- Adenoviridae --- genetics --- metabolism --- methods --- therapeutic use --- genetics

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Angiotensin II --- Peptide Fragments --- Renin-Angiotensin System --- Gene Transfer Techniques. --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Genetic Therapy --- Transgenes --- physiology. --- Theses --- Gene Transfer Techniques --- physiology

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The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.

Gene Transfer Techniques. --- Genetic Engineering. --- Stem Cells. --- Transgenes. --- Transgenes --- Genetic Techniques --- Genes --- Biological Science Disciplines --- Investigative Techniques --- Natural Science Disciplines --- Genome Components --- Disciplines and Occupations --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Genome --- Genetic Structures --- Genetic Phenomena --- Phenomena and Processes --- Physiology --- Gene Transfer Techniques --- Biology --- Health & Biological Sciences --- Genetics --- Biomedical Engineering --- Transgenic animals. --- Gene targeting --- Targeting, Gene --- Chemistry. --- Gene expression. --- Gene therapy. --- Medical genetics. --- Genetic engineering. --- Gene Therapy. --- Gene Function. --- Gene Expression. --- Genetic engineering --- Animals --- Transgenic organisms --- Animal genetic engineering --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Therapy, Gene --- Therapeutics --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Genetic regulation --- Genetic aspects --- Expression

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Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

Cloning, Molecular --- Gene Transfer Techniques --- Cells, Cultured --- Gene Targeting --- Mammals --- Transfection --- Animal cell biotechnology --- Cellules --- cytology --- methods --- genetics --- Biotechnologie --- Gene Transfer Techniques. --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Genetic Therapy --- Transgenes --- Transfection. --- Animal cell biotechnology. --- Animal cells in biotechnology --- Animal biotechnology --- Genetic transformation --- Nucleic acids --- Viral genetics --- Cloning, Molecular. --- cytology. --- methods. --- genetics. --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Gene therapy --- Molecular genetics --- Thérapie génique --- Gene therapy. --- Molecular genetics. --- Genetic Therapy. --- Molecular Biology. --- Gene Transfer Techniques. --- Gene Delivery Systems --- Gene Transfer Technique --- Transgenesis --- Delivery System, Gene --- Delivery Systems, Gene --- Gene Delivery System --- Technique, Gene Transfer --- Techniques, Gene Transfer --- Transfer Technique, Gene --- Transfer Techniques, Gene --- Biochemical Genetics --- Biology, Molecular --- Genetics, Biochemical --- Genetics, Molecular --- Molecular Genetics --- Biochemical Genetic --- Genetic, Biochemical --- Genetic, Molecular --- Molecular Genetic --- DNA Therapy --- Gene Therapy, Somatic --- Genetic Therapy, Gametic --- Genetic Therapy, Somatic --- Therapy, DNA --- Therapy, Gene --- Therapy, Somatic Gene --- Gene Therapy --- Somatic Gene Therapy --- Gametic Genetic Therapies --- Gametic Genetic Therapy --- Genetic Therapies --- Genetic Therapies, Gametic --- Genetic Therapies, Somatic --- Somatic Genetic Therapies --- Somatic Genetic Therapy --- Therapies, Gametic Genetic --- Therapies, Genetic --- Therapies, Somatic Genetic --- Therapy, Gametic Genetic --- Therapy, Genetic --- Therapy, Somatic Genetic --- Genetic Therapy --- Gene Therapy Techniques --- Molecular Biology --- Genetic engineering --- Therapeutics --- Transgenes --- Genetic Phenomena --- Gene Transfer Techniques --- Genetic Services --- Genes, Transgenic, Suicide --- Genetics --- Molecular biology --- Thérapie génique