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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple persp

Mitochondria. --- Phosphoprotein phosphatases. --- Protein kinases. --- Subcellular Fractions --- Nervous System --- Cells --- Organelles --- Metabolic Diseases --- DNA, Circular --- Cytoplasmic Structures --- DNA --- Cellular Structures --- Anatomy --- Nutritional and Metabolic Diseases --- Diseases --- Nucleic Acids --- Cytoplasm --- Intracellular Space --- Nucleic Acids, Nucleotides, and Nucleosides --- Chemicals and Drugs --- Mitochondria --- Mitochondrial Diseases --- DNA, Mitochondrial --- Neurons --- Protein kinase --- Protein phosphotransferases --- Phosphoprotein phosphohydrolases --- Protein phosphatases --- Protein phosphoesterases --- Protein phosphohydrolases --- Chondriosomes --- Phosphotransferases --- Phosphatases --- Cell organelles --- Protoplasm

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Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics. Included protocols utilize several model organisms, including yeast, Drosophila, and various plant and human cells. Protein Phosphatase Protocols provides a concise introduction to protein phosphatase research, with particular attention to phosphatase families. Assays for identification, quantification, purification, and functional characterization are presented throughout in specific detail.

Phosphoprotein phosphatases --- Phosphoprotein Phosphatases --- analysis --- isolation and purification --- Phosphatases. --- Phosphoprotein phosphatases -- Laboratory manuals. --- Phosphoric Monoester Hydrolases --- Phosphoesterases --- Phosphohydrolases --- Phosphoprotein phosphohydrolases --- Protein phosphatases --- Protein phosphoesterases --- Protein phosphohydrolases --- Esterases --- Phosphatases --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition --- Phosphoprotein phosphatases - Laboratory manuals --- Phosphoprotein Phosphatases - analysis --- Phosphoprotein Phosphatases - isolation and purification

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In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes.The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is rele

Biochemistry -- Periodicals. --- Enzymes -- Periodicals. --- Enzymes. --- Mitochondria. --- Mitochondrial pathology. --- Mitochondrial Diseases --- Phosphoprotein Phosphatases --- Mitochondria --- Protein Kinases --- Subcellular Fractions --- Phosphoric Monoester Hydrolases --- Organelles --- Metabolic Diseases --- Phosphotransferases (Alcohol Group Acceptor) --- Cellular Structures --- Nutritional and Metabolic Diseases --- Phosphotransferases --- Cytoplasmic Structures --- Esterases --- Hydrolases --- Diseases --- Cells --- Transferases --- Cytoplasm --- Enzymes --- Anatomy --- Intracellular Space --- Enzymes and Coenzymes --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Phosphoprotein phosphatases. --- Protein kinases. --- Active oxygen. --- Electron transport. --- Phosphoprotein phosphohydrolases --- Protein phosphatases --- Protein phosphoesterases --- Protein phosphohydrolases --- Protein kinase --- Protein phosphotransferases --- Oxygen radicals --- Reactive oxygen --- Singlet oxygen --- Superperoxide anion --- Chondriosomes --- Phosphatases --- Electrons --- Energy-band theory of solids --- Free electron theory of metals --- Transport theory --- Free radicals (Chemistry) --- Ionization of gases --- Oxygen --- Cell organelles --- Protoplasm