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This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.
Microbis --- Hibridació --- Fluorescència --- Citogenètica --- Manuals de laboratori --- Tècniques de laboratori --- Genètica cel·lular --- Citologia --- Genètica --- Citogenètica humana --- Mapatge cromosòmic --- Luminescència --- Radioactivitat --- Immunofluorescència --- Microscòpia de fluorescència --- Efecte Mössbauer --- Enllumenat fluorescent --- Espectroscòpia de fluorescència --- Hibridació genètica --- Hibridització --- Herència (Biologia) --- Millora de les espècies --- Espècies (Biologia) --- Lleis de Mendel --- Microorganismes --- Gèrmens --- Organismes microscòpics --- Microbiologia agrícola --- Acritarcs --- Bacteris --- Microalgues --- Microbiota --- Microorganismes extremòfils --- Microorganismes patògens --- Perífiton --- Probiòtics --- Procariotes --- Protozous --- Virus --- Microbiologia --- Microscòpia --- Hibridisme --- Cytology. --- Microbiology. --- Cell Biology. --- Microbial biology --- Biology --- Microorganisms --- Cell biology --- Cellular biology --- Cells
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Over the last few decades, the study of microbial biofilms has been gaining interest among the scientific community. These microbial communities comprise cells adhered to surfaces that are surrounded by a self-produced exopolymeric matrix that protects biofilm cells against different external stresses. Biofilms can have a negative impact on different sectors within society, namely in agriculture, food industries, and veterinary and human health. As a consequence of their metabolic state and matrix protection, biofilm cells are very difficult to tackle with antibiotics or chemical disinfectants. Due to this problem, recent advances in the development of antibiotic alternatives or complementary strategies to prevent or control biofilms have been reported. This book includes different strategies to prevent biofilm formation or to control biofilm development and includes full research articles, reviews, a communication, and a perspective.
antibiofilm --- antimicrobial agent --- bacteria --- fungi --- polymicrobial biofilm --- microalga --- free fatty acids --- encapsulation --- biofilm --- chronic wounds --- host response --- S100A8/A9 --- dental plaque --- quorum sensing --- microbial resistance --- bacterial adhesion --- blocking effect --- hydrodynamics --- parallel plate flow cell --- carbon nanotubes --- poly(dimethylsiloxane) --- adhesion --- Escherichia coli --- Biofilm --- Public Engagement --- Outreach --- Control Strategies --- Oral Biofilm --- TiO2 nanofibers --- electrospinning --- biofilm prevention and control --- multidrug-resistant bacteria --- biomedical application --- biofilms --- biofilm inhibition --- dental implants --- peri-implantitis --- polyether-ether-ketone --- Pseudomonas aeruginosa --- Candida albicans --- mixed-species biofilm analysis --- flow cytometry --- bacteriophage therapy --- prosthesis related infections --- hardware infections --- left ventricular assist devices --- Acinetobacter baumannii --- antibiotic resistance --- antibiotic tolerance --- persister --- intraspecies community --- EPS matrix --- peptide nucleic acid-fluorescence in situ hybridization --- urinary tract infections --- catheter-associated urinary tract infections --- confocal laser scanning microscopy --- recalcitrance --- biofilm control --- Klebsiella pneumoniae --- KPC and OXA-48-like carbapenemases --- Galleria mellonella infection model --- linear oligoethyleneimine hydrochloride --- bacteriophage --- endotracheal tube --- n/a
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