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Stem cells --- Cloning --- Stem Cells. --- Cloning, Organism. --- Cloning, Molecular. --- Biotechnology. --- Cloning. --- Stem cells. --- Histology. Cytology --- stamcellen --- klonen --- Life Sciences --- Biology --- embryonic stem cells --- adult stem cells --- blastocysts --- cord blood stem cells --- therapeutic cloning --- ethical issues --- medical ethics

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Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. ... The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.

polymers --- DNA (deoxyribonucleic acid) --- moleculaire biologie --- genetische manipulatie --- klonen --- Molecular biology --- Chemical laboratory practice --- polymeren --- Macromolecules --- Molecular cloning --- Cloning, Molecular --- Clonage moléculaire --- Laboratory manuals. --- Manuels de laboratoire --- ADN --- DNA --- molecular cloning --- genes --- technique de laboratoire --- laboratory techniques --- Vecteur génétique --- Genetic vectors --- Plasmide --- plasmids --- PCR --- Bioinformatique --- Bioinformatics --- Transfection --- transfection --- Expression des gènes --- gene expression --- Interaction génique --- gene interaction --- Cloning, Molecular. --- Molecular Cloning --- Cosmids --- Cloning, Organism --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- 542 --- clonen --- laboratoriumtechnieken --- groene chemie --- Clonage moléculaire --- Klonen --- Moleculaire genetica --- Genetische manipulatie --- DNA. --- transfection. --- bio-informatica --- mutageniteit --- oligonucleotides --- microarrays --- gisten --- Monograph --- Molecular cloning - Laboratory manuals

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Biotechnology --- Biotechnologie --- Periodicals --- Périodiques --- Biotechnology. --- Genetic Engineering. --- Genetic Techniques. --- 66.098 --- Genetic Technic --- Genetic Technics --- Genetic Technique --- Technic, Genetic --- Technics, Genetic --- Technique, Genetic --- Techniques, Genetic --- Genetics --- Genetic Counseling --- Genetic Processes --- Engineering, Genetic --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Biotechnologies --- Biological processes. Biotechnology --- methods --- Life Sciences --- Biology --- Genetic Phenomena --- 66.098 Biological processes. Biotechnology --- Genetic Engineering --- Genetic Techniques --- biotechnologie --- Chemical engineering --- Genetic engineering --- Bioengineering --- biotechnology --- applied microbiology --- biomedical research methods

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Interferon --- Genetic engineering --- Recombinant proteins --- Genetic Engineering. --- Interferons. --- Recombinant Proteins. --- Genetic engineering. --- Interferon. --- Recombinant proteins. --- Agriculture Sciences --- Chemistry --- Engineering --- Life Sciences --- Agricultural Biotechnology --- Soil Chemistry, Microbiology, Fertility & Fertilizers --- Biochemistry --- Biotechnology --- biotechnology --- biomedicine --- biosciences --- microbiology --- molecular genetics --- immunology --- Genetically engineered proteins --- Proteins --- Interferons --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Proteins, Biosynthetic --- Proteins, Recombinant DNA --- Biosynthetic Proteins --- DNA Recombinant Proteins --- DNA Proteins, Recombinant --- Proteins, DNA Recombinant --- Proteins, Recombinant --- Recombinant DNA Proteins --- Recombinant Proteins, DNA --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- Recombinant molecules --- Antineoplastic agents --- Antiviral agents --- Glycoproteins --- Lymphokines --- Genetic recombination --- Transgenic organisms --- Bioreactors --- Viral Interference --- Cloning, Molecular --- DNA, Recombinant --- Industrial Microbiology --- Artificial Gene Fusion --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- biotechnologie --- Biosynthetic Protein --- Recombinant Protein --- Protein, Biosynthetic --- Protein, Recombinant

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Now in its sixth edition, Principles of Gene Manipulation provides an excellent introduction to the area of genetic engineering of plants, animals and microbes for advanced level undergraduates, with a basic understanding of genetics. This classic textbook has been substantially updated and revised to reflect the rapid advances that have been made in the core technologies in the seven years since the last edition. Furthermore, to put these technologies into context, the final chapter has been structured into six themes: 
· nucleic acids as diagnostic tools 
· new drugs and new therapies for genetic diseases 
· combating infectious disease 
· protein engineering 
· metabolic engineering 
· modern plant breeding 
A website is now available to complement this text, at www.blackwellpublishing.com/primrose


577.2 --- 575.08:577.21 --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- Genetic engineering, genetic manipulation, recombinant DNA --- 577.2 Molecular bases of life. Molecular biology --- Molecular bases of life. Molecular biology --- genen --- Chromosome Mapping. --- Base Sequence. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic surgery --- Genetic recombination --- Transgenic organisms --- Human Genome Project --- Genome --- DNA Sequences --- DNA Sequence --- Nucleotide Sequence --- RNA Sequence --- Base Sequences --- Nucleotide Sequences --- RNA Sequences --- Sequence, Base --- Sequence, DNA --- Sequence, Nucleotide --- Sequence, RNA --- Sequences, Base --- Sequences, DNA --- Sequences, Nucleotide --- Sequences, RNA --- Nucleotides --- Genome Mapping --- Gene Mapping --- Linkage Mapping --- Chromosome Mappings --- Gene Mappings --- Genome Mappings --- Linkage Mappings --- Mapping, Chromosome --- Mapping, Genome --- Mapping, Linkage --- Mappings, Chromosome --- Mappings, Gene --- Mappings, Genome --- Mappings, Linkage --- Genetic Markers --- Gene Order --- Synteny --- Intervention, Genetic --- Genetic Intervention --- Genetic Interventions --- Interventions, Genetic --- DNA, Recombinant --- Industrial Microbiology --- Organisms, Genetically Modified --- Animals, Genetically Modified --- Plants, Genetically Modified --- Genetic Engineering. --- Genetic engineering --- Base Sequence --- Chromosome Mapping --- Genetic Engineering --- Genomics --- Genetic intervention --- Biotechnology --- Cloning, Molecular --- Artificial Gene Fusion --- Recombinant DNA Research --- Recombination Joint --- Genes, Spliced --- Recombinant DNA --- DNA Research, Recombinant --- Joint, Recombination --- Research, Recombinant DNA --- Spliced Genes --- DNA Transposable Elements --- Genetic Vectors --- Genomic Library --- Gene Library --- Mapping, Gene --- Cytogenetics --- Cytogenetic Analysis --- Genes --- moleculaire biologie --- Molecular biology --- genetische manipulatie --- Genetics --- Comparative Genomics --- Comparative Genomic --- Genomic, Comparative --- Genomics, Comparative --- Genetic engineering. --- Dna, recombinant. --- genetic engineering --- besmettelijke ziekten