Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

General microbiology --- Biology --- Immunology. Immunopathology --- immunologie --- N (stikstof) --- koolstofkringloop --- SARS (severe acute respiratory syndrome) --- CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) --- biologie --- microbiologie --- analyse (chemie)

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

CRISPR-Cas is a recently discovered defense system which protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR-Cas immune systems has been identified in at least half of the available prokaryotic genomes. On-going biochemical and functional analyses have resulted in substantial insight into the functions and possible applications of these fascinating systems, although many secrets remain to be uncovered. In this book, experts summarize the state of the art of this exciting field.

Genetics --- General microbiology --- Biology --- Medical microbiology, virology, parasitology --- medische microbiologie --- genomen (geneeskunde) --- medische genetica --- CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) --- biologie --- microbiologie --- genetica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Genome Editing Techniques are seen to be at the frontier of current research in the field of emerging biotechnologies. The latest revolutionary development, the so-called CRISPR technology, represents a paradigmatic example of the ambiguity of such techniques and has resulted in an international interdisciplinary debate on whether or not it is necessary to ban the application of this technique by means of a moratorium on its use for human germline modifications, particularly in human embryos in the reproduction process. However, given that other germline engineering techniques like mitochondrial (mt) DNA transfer techniques are already permitted and applied, the question arises what lies at the root of the apparent social unease about the modification of the human germline by Genome Editing Techniques like CRISPR. Against this background, the book seeks to make a substantial contribution to the current debate about a responsible and participatory framework for research on emerging biotechnologies by analysing underlying perceptions, attitudes, arguments and the reasoning on Genome Editing Techniques. Contents Mapping the current legal framing Theoretical Challenges Orientation markers for a Governance Frame in dealing with Genome Editing Target Groups Scientist, Policy Makers, Lecturers and students in the fields of Ethics, Philosophy, Theology, Law, Social and Political Science. The Editors Matthias Braun is assistant Professor at the Chair of Systematic Theology II (Ethics), Friedrich-Alexander University Erlangen-Nuremberg. Hannah Schickl is research assistant in the Interdisciplinary Research Group “Gene Technology Report”, Berlin-Brandenburg Academy of Sciences and Humanities. Peter Dabrock is Professor of Systematic Theology/Ethics at the Friedrich-Alexander University Erlangen-Nuremberg and Chair of the German Ethics Council.

Philosophy --- Professional ethics. Deontology --- General ethics --- Engineering sciences. Technology --- CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) --- ethiek --- filosofie --- biotechnologie --- bio-ethiek --- medische ethiek --- ingenieurswetenschappen

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.

Genetics --- Histology. Cytology --- Biology --- Animal genetics. Animal evolution --- Pharmacology. Therapy --- Human medicine --- Manufacturing technologies --- farmacologie --- CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) --- genomics --- stamcellen --- biologie --- geneeskunde --- genetica --- stamceltransplantatie --- histologie

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Bioremediation refers to the clean‐up of pollution in soil, groundwater, surface water, and air using typically microbiological processes. It uses naturally occurring bacteria and fungi or plants to degrade, transform or detoxify hazardous substances to human health or the environment. For bioremediation to be effective, microorganisms must enzymatically attack the pollutants and convert them to harmless products. As bioremediation can be effective only where environmental conditions permit microbial growth and action, its application often involves the management of ecological factors to allow microbial growth and degradation to continue at a faster rate. Like other technologies, bioremediation has its limitations. Some contaminants, such as chlorinated organic or high aromatic hydrocarbons, are resistant to microbial attack. They are degraded either gradually or not at all, hence, it is not easy to envisage the rates of clean-up for bioremediation implementation. Bioremediation represents a field of great expansion due to the important development of new technologies. Among them, several decades on metagenomics expansion has led to the detection of autochthonous microbiota that plays a key role during transformation. Transcriptomic guides us to know the expression of key genes and proteomics allow the characterization of proteins that conduct specific reactions. In this book we show specific technologies applied in bioremediation of main interest for research in the field, with special attention on fungi, which have been poorly studied microorganisms. Finally, new approaches in the field, such as CRISPR-CAS9, are also discussed. Lastly, it introduces management strategies, such as bioremediation application for managing affected environment and bioremediation approaches. Examples of successful bioremediation applications are illustrated in radionuclide entrapment and retardation, soil stabilization and remediation of polycyclic aromatic hydrocarbons, phenols, plastics or fluorinated compounds. Other emerging bioremediation methods include electro bioremediation, microbe-availed phytoremediation, genetic recombinant technologies in enhancing plants in accumulation of inorganic metals, and metalloids as well as degradation of organic pollutants, protein-metabolic engineering to increase bioremediation efficiency, including nanotechnology applications are also discussed.

General biochemistry --- Eumycetes --- Botany --- Insects. Springtails --- Electrical engineering --- Pedology --- Agriculture. Animal husbandry. Hunting. Fishery --- Biochemical engineering --- Biotechnology --- bodemkunde --- systematische plantkunde --- mycologie --- nanotechniek --- bodembescherming --- proteomics --- CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) --- biochemie --- bevolking --- biotechnologie --- grondwater --- insecten --- planten --- enzymen