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The FISH Handbook for Biological Wastewater Treatment provides all the required information for the user to be able to identify and quantify important microorganisms in activated sludge and biofilms by using fluorescence in situ hybridization (FISH) and epifluorescence microscopy. It has for some years been clear that most microorganisms in biological wastewater systems cannot be reliably identified and quantified by conventional microscopy or by traditional culture-dependent methods such as plate counts. Therefore, molecular biological methods are vital and must be introduced instead of, or in addition to, conventional methods. At present, FISH is the most widely used and best tested of these methods. This handbook presents all relevant information from the literature and, based on the extensive experience of the authors, advice and recommendations are given for reliable FISH identification and quantification.

Sewage --- Fluorescence in situ hybridization. --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization --- Biological nutrient removal (Sewage treatment) --- BNR (Sewage treatment) --- Bioremediation --- Sewage disposal --- Purification --- Biological treatment. --- Biological treatment

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In the past three decades, a stream of criminological inquiry has emerged which explores, measures, and theorizes crimes and harms to the environment at the micro-, mezzo-, and macro-levels. This “green criminology”, as it has come to be known, has widened the criminological gaze to consider crimes and harms committed against air, land (from forests to wetlands), nonhuman animals, and water in local, regional, national, and international areas or arenas. Accordingly, green criminology has endeavored to understand the causes and consequences of air and water pollution, biodiversity loss, climate change, corporate environmental crime (e.g., illegal waste disposal), food production and distribution, resource extraction and exploitation, and wildlife trade and trafficking, while also exploring potential responses to these issues. This book seeks to introduce the green criminological perspective to a broader social science audience. Recognizing that green criminology is not the first social science to explore the phenomena and harms at the intersections of humanity and ecology, this book offers an introduction to some of the unique insights developed over nearly 30 years of green criminological thought and scholarship to students, professors, researchers, and practitioners working in the fields of anthropology, economics, environmental humanities, environmental sociology, geography, history, and political ecology. This book contains contributions from researchers in green criminology from around the world, including early- and mid-career scholars, as well as more established voices in the field—all of whom are dedicated to exposing, understanding, and ultimately hoping to thwart further environmental degradation and despoliation.

biogeography --- ciliates --- Paramecium quindecaurelia --- cytochrome C oxidase subunit I gene --- sibling species --- species concept in protists --- bacterial symbionts --- symbiosis --- intranuclear bacteria --- Holospora --- Gortzia --- Paramecium --- Micractinium tetrahymenae --- Tetrahymena --- Utricularia --- facultative endosymbiosis --- ciliate-algae symbiosis --- Chlorella variabilis --- Micractinium conductrix --- diagnostic PCR --- ciliate–algae symbiosis --- Holospora-like bacteria --- host–parasite interactions --- 16S rRNA gene --- full-cycle rRNA approach --- TEM --- fluorescence in situ hybridization --- algal-ciliate symbiosis --- mycosporine-like amino acids --- Pelagodileptus trachelioides --- planktonic freshwater ciliates --- Stokesia vernalis --- Vorticella chlorellata --- Chlorella --- endosymbiosis --- intracellular algae --- Micractinium --- photobiont --- infection --- syngen --- n/a --- host-parasite interactions

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Supernumerary B chromosomes (Bs) are dispensable genetic elements found in thousands of species of plants and animals, and some fungi. Since their discovery more than a century ago, they have been a source of puzzlement, as they only occur in some members of a population and are absent from others. When they do occur, they are often harmful, and in the absence of “selfishness”, based on mechanisms of mitotic and meiotic drive, there appears to be no obvious reason for their existence. Cytogeneticists have long wrestled with questions about the biological existence of these enigmatic elements, including their lack of any adaptive properties, apparent absence of functional genes, their origin, sequence organization, and co-evolution as nuclear parasites. Emerging new technologies are now enabling researchers to step up a gear, to look enthusiastically beyond the previous limits of the horizon, and to uncover the secrets of these “silent” chromosomes. This book provides a comprehensive guide to theoretical advancements in the field of B chromosome research in both animal and plant systems.

parent-of-origin effects --- fluorescent in situ hybridization --- coverage ratio analysis --- n/a --- ribosomal DNA --- reactivation --- cytogenetics --- epigenetics --- heterochromatin --- interphase nucleus --- whole genome resequencing --- transmission --- grasshoppers --- genome instability --- dot-like (micro) Bs --- ?s --- B chromosome --- supernumerary elements --- transcription of heterochromatin --- maternal X chromosome --- supernumerary chromosome --- population analysis --- supernumerary --- repeat clusters --- extra chromosomes --- genes --- tandem repeats --- B morphotypes --- repetitive DNA --- repetitive elements --- DNA copy number variation --- chromosome polymorphism --- satellite DNA --- mammals --- maize B chromosome --- additional chromosomes --- inactivation --- drive --- B chromosomes --- FISH (fluorescence in situ hybridisation) --- organelle DNA --- Orthoptera --- origin --- supernumerary chromosomes --- karyotype evolution --- GISH (genomic in situ hybridisation) --- DNA composition --- de novo centromere formation --- genomics --- paternal X chromosome --- euchromatin degradation --- supernumerary chromosomal segments (SCS) evolution --- centromere --- sSMC --- Prospero autumnale complex --- next-generation sequencing --- Drosophila --- host/parasite interaction --- Apodemus peninsulae --- genome evolution --- evolution --- teleost --- chromosome evolution --- microdissected DNA probes --- controlling element --- mobile element --- RNA-Seq --- karyotypes --- karyotypic characteristics --- RepeatExplorer