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In biofilms, microorganisms are able to communicate together and assemble by themselves, creating a consortium with different properties from the original free-floating microorganisms. In fact, biofilm cells bind strongly to a living or non-living surface, enclosed in a self-produced extracellular matrix that is composed of extracellular polymeric substances. One benefit of this lifestyle is the increased resistance or tolerance to antimicrobial agents (e.g., antibiotics). Hence, research on the development of alternative strategies to prevent and control biofilms is highly relevant for society in terms of human health, industry and the environment. Different approaches to prevent or control biofilms using antibiotic alternative strategies were submitted to this Special Issue.

antibacterial drug --- vitamin B12 --- antisense oligonucleotides --- nucleic acid mimics --- LNA --- 2′OMe --- biocidal particles --- functionalization --- benzalkonium chloride --- Escherichia coli --- antimicrobial activity --- antimicrobial resistance --- natural alkylglycerols --- ether lipids --- 1-O-alkyl-sn-glycerols --- antibiofilm activity --- quorum sensing inhibition --- stainless steel --- polypropylene --- organic matter --- microbial resistance --- peracetic acid --- biofilms --- prosthesis-related infections --- heat shock --- ciprofloxacin --- antibacterial agents --- E. coli biofilms --- food contact surfaces --- biocontrol --- bacteriophages --- aluminum nitride --- composite --- antibacterial --- mechanical --- thermal --- cellulose acetate --- CAPE --- Candida --- antifungal --- biofilm --- apoptosis --- urinary tract devices --- probiotics --- Lactobacillus plantarum --- Lactobacillus rhamnosus --- displacement --- oral biofilm --- dental implants --- titanium implants --- antimicrobial --- surface coating --- anti-fouling --- n/a --- 2'OMe