Abstract | Keywords | Export | Availability | Bookmark

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Speci?c binding of a ligand to a receptor is a key step in a variety of biol- ical processes, such as immune reactions, enzyme cascades, or intracellular transport processes. The ligand-receptor terminology implies that the rec- tor molecule is signi?cantly larger than the ligand, and the term bioactive conformation  usually characterizes the conformation of a ligand when it is bound to a receptor. In a more general sense, bioactive conformation applies toanymoleculeinabiologicallyrelevantboundstateregardlessofsizecons- erations. Mostofthecontributions tothisbookaddressligandsthat aremuch smaller than their receptors. X-ray crystallography and high resolution NMR spectroscopy are the two main experimental techniques used to study bioactive conformations. The- fore,the twovolumes ofthisbookcover approachesthat use either ofthetwo techniques, or a combination thereof. The combination of X-ray crystallog- phy and NMR spectroscopy is particularly useful when a crystal structure of areceptorprotein,butnotofthereceptorprotein-ligandcomplex,isavailable. Anumberofexperimentaltechniquestoanalyzethebioactiveconformationof aligandwithNMRarebasedontheobservationoftheresonancesignalsofthe free ligand that is in exchange with the bound ligand. Several chapters focus onsuchapproachesthat rangefromclassical transferredNOEexperiments, totransferred dipolar couplings,toSTD (saturation transfer difference) NMR techniques. Incaseswhere tightbinding inthesub-nanomolar rangeprevents the analysis of the bioactive conformation via free ligand signals, the ligand- proteincomplexhas tobeanalyzed withproteinNMR-based techniques orby crystallography.Sincethisareahasbeenthesubjectofmanyreviewsandmo- graphsitwill not be covered here in particular detail. As a unifying theme, all contributionstargetthequestionofhowmolecular recognitionofbiologically active molecules is achieved on the atomic scale. Depending on the research topic the results from these studies have a strong impact not only in basic research but also in several ?elds of application ranging frompharmaceutical applications tothe use of biomolecules as, for example, cryoprotectants.

Chemistry. --- Organic Chemistry. --- Biochemistry, general. --- Physical Chemistry. --- Medicinal Chemistry. --- Analytical Chemistry. --- Analytical biochemistry. --- Chemistry, Organic. --- Physical organic chemistry --- Biochemistry. --- Chimie --- Biochimie analytique --- Chimie organique --- Chimie organique physique --- Biochimie --- Conformational analysis --- Bioactive compounds --- Ligand binding (Biochemistry) --- Molecular Conformation --- Binding Sites --- Ligands --- Nuclear Magnetic Resonance, Biomolecular --- Protein Binding --- Analysis --- Chemistry --- Biochemistry --- Physical Sciences & Mathematics --- 577.354.3 --- Chemoreception --- 577.354.3 Chemoreception --- Conformational analysis. --- Analysis. --- Binding, Ligand (Biochemistry) --- Analysis, Conformational --- Biologically active compounds --- Compounds, Bioactive --- Compounds, Biologically active --- Compounds, Physiologically active --- Physiologically active compounds --- Analytical chemistry. --- Organic chemistry. --- Physical chemistry. --- Medicinal chemistry. --- Dye-ligand affinity chromatography --- Radioligand assay --- Molecular rotation --- Chemicals --- Chemistry, Physical organic. --- Analytic biochemistry --- Chemistry, Analytic --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Organic chemistry --- Composition --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Bioactive compounds - Analysis