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This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.
Proteins --- Protein Biosynthesis. --- Combinatorial Chemistry Techniques. --- High-Throughput Screening Assays. --- Protéines --- Protéines --- Synthèse protéique. --- isolation & purification. --- Biotechnologie. --- Purification.
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Molecular biology --- Molecular Biology --- Biologie moléculaire --- Cytologie --- cytology --- Biochimie --- biochemistry --- Génétique --- genetics --- ADN --- DNA --- ARN --- RNA --- Synthèse protéique --- protein synthesis --- DNA. --- RNA. --- Molecular biology. --- Biologie moleculaire
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cancer --- anticancéreux --- innovation thérapeutique --- pétrole --- homéopathie --- médecine naturelle --- parasitisme latent du sang --- test de dépistage --- régulation de la synthèse protéique --- musique et l'organisme --- nomenklatura scientifique --- voyages spatiaux
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Peptide --- peptides --- Biochimie --- biochemistry --- Propriété physicochimique --- chemicophysical properties --- Synthèse protéique --- protein synthesis --- Acide aminé --- Amino acids --- Génie chimique --- Chemical engineering --- 577.112.6 --- 547.057 --- #WSCH:LOSH --- Synthetic peptides and polypeptides --- Organic chemistry--?.057 --- Peptides --- Peptiden --- Synthese --- Synthesis. --- Peptiden. --- Synthese. --- 547.057 Organic chemistry--?.057 --- 577.112.6 Synthetic peptides and polypeptides
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Comprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.
Molecular biology --- Enzymes --- Micro-organisme --- microorganisms --- Propriété optique --- Optical properties --- Biochimie --- biochemistry --- Génie biochimique --- biochemical engineering --- Variation génétique --- genetic variation --- Oxydation --- Oxidation --- Synthèse protéique --- protein synthesis --- Contamination biologique --- Biological contamination --- Contamination chimique --- Chemical contamination --- Surveillance de l'environnement --- Biochemistry. --- Bioluminescence assay. --- Electronic books. -- local. --- Bioluminescence assay --- Photometry --- Molecular Probe Techniques --- Luminescent Measurements --- Biosensing Techniques --- Investigative Techniques --- Chemistry Techniques, Analytical --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Human Anatomy & Physiology --- Animal Biochemistry --- Health & Biological Sciences --- Life sciences. --- Life Sciences. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Biosciences --- Sciences, Life --- Science --- Composition --- ATP luminescence assay --- Bioluminescent assay --- Biological assay --- Application --- Luciferase
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Cytology --- Molecular biology --- Genetics --- Cytologie --- Biologie moléculaire --- Génétique --- genes --- Hérédité --- genetic inheritance --- Métabolisme --- Metabolism --- Bacteria --- Virus --- viruses --- ADN --- DNA --- ARN --- RNA --- Synthèse protéique --- protein synthesis --- Développement embryonnaire --- Embryonic development --- Réaction antigène anticorps --- antigen antibody reactions --- Néoplasme --- Neoplasms --- 577.21 --- Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- 577.21 Molecular mechanism of coding, storage and realization of inheritance information. Molecular genetics. Molecular biology of the gene --- Biologie moléculaire --- Génétique --- viruses. --- DNA. --- RNA. --- Molecular biology. --- Genes, regulator
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Protéines --- Biotechnologie --- Structure --- Proteines --- Structure. --- Biotechnologie. --- Protéine --- proteins --- Acide aminé --- Amino acids --- Structure chimique --- chemical structure --- Minéralogie --- Mineralogy --- Spectroscopie RMN --- NMR spectroscopy --- Solution --- solutions --- Hémoglobine --- Haemoglobin --- Insuline --- Insulin --- Anticorps --- antibodies --- Vaccin --- Vaccines --- Enzymes --- Industrie pharmaceutique --- pharmaceutical industry --- Modèle --- Models --- Synthèse protéique --- protein synthesis --- Protéines --- Proteines - Structure --- Proteines - Biotechnologie --- Structure moleculaire --- Proteine en solution --- PROTEINS --- MOLECULAR STRUCTURE --- CRYSTAL STRUCTURE --- NUCLEAR MAGNETIC RESONANCE --- HEMOGLOBINS --- ANTIBODIES --- VACCINES --- INSULIN --- RECEPTORS --- PHARMACEUTICAL PREPARATIONS --- ENZYMES --- FOOD --- PREPARATION --- Genetic engineering. --- Proteins. --- Molecular structure. --- Biotechnology. --- Génie génétique. --- Protéines. --- Structure moléculaire. --- Technologie des protéines.
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The study of protein-nucleic acid interactions is currently one of the most rapidly growing areas of molecular biology. DNA binding proteins are at the very heart of the regulation and control of gene expression, replication, and recombination: Enzymes that recognize and either modify or cleave specific DNA sequences are equally important to the cell. Some of the techniques reported in this volume can be used to identify previously unknown DNA binding proteins from crude cell extracts. Virtually all are capable of giving direct information on the molecular basis of the interaction—the location of the DNA binding site; the strength and specificity of binding; the identities of individual groups on specific bases involved in binding; the specific amino acid residues of the protein that interact with the DNA; or the effects of protein binding on gross conformation and local structure of DNA. The recognition of DNA sequences by proteins is a complex phenomenon, involving specific hydrogen bonding contacts to the DNA bases ("direct readout") and/or interactions with the sugar-phos phate backbone ("indirect readout"). The latter interactions can also be highly specific because of sequence-dependent conformational changes in the DNA. In addition, intercalation of planar aromatic amino acid side-chains between the DNA bases can occur, most notably with single-stranded DNA binding proteins. Furthermore, when bound, many DNA binding proteins induce drastic structural changes in the DNA as an integral part of their function.
DNA-protein interactions --- ADN --- DNA --- Protéine --- proteins --- Acide nucléique --- Nucleic acids --- Protéine de liaison --- Binding proteins --- Synthèse protéique --- protein synthesis --- DNA-protein interactions. --- Electronic books. -- local. --- Protein binding. --- Genetic Techniques --- DNA-Binding Proteins --- Proteins --- Investigative Techniques --- Amino Acids, Peptides, and Proteins --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Chemicals and Drugs --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- DNA-protein binding --- Interactions, DNA-protein --- Protein-DNA interactions --- DNA-ligand interactions --- Protein binding --- Binding, Protein --- Biochemistry --- Allosteric proteins --- Radioligand assay --- DNA. --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Internationalement reconnu, Biochimie est l’ouvrage de référence incontesté dans son domaine. Au fil des éditions successives – la première datant de 1975 –, il a couvert cette discipline de façon claire et attrayante. La 7e édition de Biochimie ne déroge pas à cette règle : l’étudiant qui se lance dans la découverte de cette discipline y trouvera un langage direct et accessible, des illustrations dédiées à chaque fois à une unique notion de façon à ne pas démultiplier les informations, les rapports entre les mécanismes biochimiques et la physiologie, la santé ou les pathologies, et un développement des concepts biochimiques en lien constant avec les processus de l’Évolution. Les acquisitions les plus récentes, constamment enrichies des découvertes des chercheurs du monde entier, sont bien sûr prises en compte. Ainsi la 7e édition intègre les données les plus récentes sur l’obésité et l’épidémie de diabète provenant des nouvelles connaissances du rôle des leptines ; elle présente les avancées dans la compréhension de la régulation biochimique des gènes eucaryotes, à travers notamment les cellules souches pluripotentes induites et le rôle des microRNA ; elle met à jour les principes des techniques expérimentales – tels la spectrométrie de masse, la cristallographie aux rayons X, le séquençage de nouvelle génération ou la PCR en temps réel – afin d’apporter aux étudiants une compréhension pratique des avantages et des limites des techniques qu’ils utiliseront au laboratoire. Dans un but volontairement pédagogique, les 36 chapitres constituant cette 7e édition s’achèvent tous selon la même structure : un résumé des notions fondamentales qu’il faut retenir, une liste des mots clés et une trentaine de problèmes portant sur le chapitre qui vient d’être lu. L’ouvrage se termine par les corrigés détaillés de tous les problèmes, une sélection de références bibliographiques classées par chapitre et par thème, et un index de près de 8000 entrées.
Biochemistry --- Biochimie --- Molecular biology --- Genes --- Metabolism --- Biologie moléculaire --- biochemistry --- Génie biochimique --- biochemical engineering --- Biotechnologie --- Biotechnology --- Activité enzymatique --- Enzyme activity --- Voie biochimique du métabolisme --- Biochemical pathways --- Génétique moléculaire --- Molecular genetics --- Biosynthèse --- Biosynthesis --- Synthèse protéique --- protein synthesis --- Transformation génétique --- genetic transformation --- Study and teaching (Higher) --- Etude et enseignement (supérieur) --- Textbooks --- Manuels d'enseignement supérieur --- Manuels d'enseignement supérieur --- Biologie moléculaire. --- Textbooks. --- Études et enseignement (supérieur) --- Biochemistry. --- Molecular biology. --- Genes. --- Metabolism. --- biochemistry. --- biochemical engineering. --- Biotechnology. --- Enzyme activity. --- Biochemical pathways. --- Molecular genetics. --- Biosynthesis. --- protein synthesis. --- genetic transformation.
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General biochemistry --- Nucleic acids --- Acides nucléiques --- Nucleic Acids --- Génie génétique --- genetic engineering --- Acide nucléique --- Biochimie --- biochemistry --- Nucléotide --- Nucleotides --- chromosomes --- Clonage --- cloning --- Synthèse protéique --- protein synthesis --- Métabolisme des protéines --- Protein metabolism --- Localisation de gène --- gene location --- Transformation génétique --- genetic transformation --- Nucleic Acids. --- 577.123 --- Acids, Nucleic --- Nucleic acid metabolism --- Nucleic acids. --- Basic Sciences. Molecular Biology --- Moleculaire biologie --- Nucleinezuren --- Moleculaire biologie. --- Nucleinezuren. --- 577.123 Nucleic acid metabolism --- Acides nucléiques --- Polynucleotides --- Biomolecules --- Acides nucléiques. Biochimie. --- Nucleïnezuren. Biochemie. --- Nucleic Acid --- Acid, Nucleic --- Molecular structure. --- Molecular structure --- DNA Repair. --- DNA Replication. --- DNA, Recombinant. --- DNA Repair --- DNA Replication --- DNA, Recombinant --- Chromosomes --- Transcription, genetic --- DNA REPAIR --- DNA --- REPLICATION --- NUCLEOTIDES --- NUCLEASES --- CHROMOSOMES --- NUCLEIC ACIDS --- TEXTBOOKS --- METHODS --- M-RNA --- GENES --- RNA --- GENETIC RECOMBINATION --- METABOLISM --- FUNCTIONS --- STRUCTURES --- BIOCHEMISTRY --- BIOSYNTHESIS
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