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Protein microarrays

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RNA --- Molecular diagnosis. --- Protein microarrays. --- Polymerase chain reaction. --- Reverse transcriptase. --- Aviation medicine --- Purification. --- Technological innovations

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Protein arrays make possible the detection and quantitation of many proteins simultaneously, thus enabling researchers to ask fundamental questions about biological processes and to discover biomarkers that can be used diagnostically. In Protein Arrays: Methods and Protocols, innovative experimentalists describe in detail the methods they have developed to synthesize and construct protein arrays for basic and clinical research. The authors present protocols to create and immobilize the capture substrate-the first task in designing a protein array-using a variety of affinity capture reagents, including antibodies, peptides, aptamers, biotin, chemical reagents, and chromatographic substrates. Once synthesized, these arrays can be used to analyze protein-protein interactions and posttranslational modifications, such as phosphorylation, as well as to discover and characterize potential diagnostic markers. Protocols to accomplish these tasks are also presented. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Diverse and highly practical, Protein Arrays: Methods and Protocols offers basic and clinical investigators a broad spectrum of approaches to the generation of protein arrays, as well as their uses in biomarkers discovery, in assay development, in clinical sample testing, in signal transduction analysis and characterization, and for creating the next generation of molecular tools.

Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Protein microarrays. --- Proteins --- Analysis.

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This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays. Since their development in 2001, cell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody arrays. The surface used to coat the glass slides has also been significantly improved to allow non-adherent cells to bind to the arrays.

Protein microarrays --- Life sciences --- Statistical methods. --- Biology. --- Biosciences --- Sciences, Life --- Science --- Microarrays, Protein --- Peptide biochips --- Peptide microarrays --- Protein biochips --- Biochips --- Immobilized proteins --- Cytology. --- DNA microarrays. --- Human genetics. --- Cell Biology. --- Microarrays. --- Human Genetics. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- DNA biochips --- Microarrays, DNA --- Immobilized nucleic acids --- Cell biology.

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The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.

DNA microarrays. --- Protein microarrays. --- Microarrays, Protein --- Peptide biochips --- Peptide microarrays --- Protein biochips --- Biochips --- Immobilized proteins --- DNA biochips --- Microarrays, DNA --- Immobilized nucleic acids --- Medicine. --- Biomedical engineering. --- Nanotechnology. --- Chemistry, Physical organic. --- Biomedicine general. --- Biological and Medical Physics, Biophysics. --- Biomedical Engineering and Bioengineering. --- Physical Chemistry. --- Medicine/Public Health, general. --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Molecular technology --- Nanoscale technology --- High technology --- Health Workforce --- Biophysics. --- Biological physics. --- Physical chemistry. --- Biomedicine, general. --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Chemistry --- Biological physics --- Biology --- Physics