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"Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline."--

Nervous system --- Phenotype. --- Stem cells --- Diseases --- Treatment. --- Research. --- Phenotypes --- Genetics --- Genotype-environment interaction --- Induced pluripotent stem cells. --- Neurons. --- Induced Pluripotent Stem Cells --- Neurons --- Phenotype --- Nervous System Diseases --- therapy --- Induced Pluripotent Stem Cells. --- therapy.

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Since their development a decade ago, human induced pluripotent stem cells (iPSC) have revolutionized the study of human disease, given rise to regenerative medicine technologies, and provided exceptional opportunities for pharmacologic research. These cells provide an essentially unlimited supply of cell types that are difficult to obtain from patients, such as neurons or cardiomyocytes, or are difficult to maintain in primary cell culture. iPSC can be obtained from patients afflicted with a particular disease but, in combination with recently developed gene editing techniques, can also be modified to generate disease models. Moreover, the new techniques of 3 Dimensional printing and materials science facilitate the generation of organoids that can mirror organs under disease conditions. These properties make iPSC powerful tools to study how diseases develop and how they may be treated. In addition, iPSC can also be used to treat conditions in which the target cell population has been lost and such regenerative approaches hold great promise for currently untreatable diseases, including cardiac failure or photoreceptor degenerations.

Neurosciences. --- Regenerative medicine. --- Stem cells. --- Neuroscience. --- Regenerative Medicine and Tissue Engineering. --- Stem Cell Biology. --- Drugs --- Research. --- Induced Pluripotent Stem Cells. --- Drug Discovery.

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Current Topics in iPSCs provides a deep analysis of the underlying fundamentals that support short and mid-term developments and milestones in the business of mesenchymal stem cell therapies. This volume explores the next frontier of MSC therapies and how the transformational potential of therapeutic adult cells will be realised in all therapy areas. The impacts of clinical and economic benefits are dissected throughout each of the chapters. Written by thought leaders in the field for those curious about the interface of science and business.

Mesenchymal stem cells. --- Stem cells --- Mesenchymal Stem Cells. --- Stem Cells. --- Stem Cell Research. --- Adult Stem Cell Research --- Embryonic Stem Cell Research --- Research, Stem Cell --- Researchs, Stem Cell --- Stem Cell Researchs --- Stem Cells --- Embryo Research --- Colony-Forming Unit --- Colony-Forming Units --- Mother Cells --- Progenitor Cells --- Cell, Mother --- Cell, Progenitor --- Cell, Stem --- Cells, Mother --- Cells, Progenitor --- Cells, Stem --- Colony Forming Unit --- Colony Forming Units --- Mother Cell --- Progenitor Cell --- Stem Cell --- Cell Self Renewal --- Stem Cell Research --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Bone marrow stromal cells --- Marrow stromal cells --- MSCs (Mesenchymal stem cells) --- Stromal cells, Bone marrow --- Stromal cells, Marrow --- Multipotent stem cells --- Therapeutic use. --- Adipose Tissue-Derived Mesenchymal Stem Cells --- Adipose Tissue-Derived Mesenchymal Stromal Cells --- Adipose-Derived Mesenchymal Stem Cells --- Adipose-Derived Mesenchymal Stromal Cells --- Bone Marrow Mesenchymal Stem Cells --- Bone Marrow Stromal Cell --- Bone Marrow Stromal Cells --- Bone Marrow Stromal Cells, Multipotent --- Bone Marrow Stromal Stem Cells --- Mesenchymal Progenitor Cell --- Mesenchymal Progenitor Cells --- Mesenchymal Stem Cell --- Mesenchymal Stem Cells, Adipose-Derived --- Mesenchymal Stromal Cells, Multipotent --- Multipotent Bone Marrow Stromal Cells --- Multipotent Mesenchymal Stromal Cells --- Stem Cells, Mesenchymal --- Wharton Jelly Cells --- Wharton's Jelly Cells --- Adipose Tissue-Derived Mesenchymal Stem Cell --- Adipose-Derived Mesenchymal Stem Cell --- Bone Marrow Mesenchymal Stem Cell --- Mesenchymal Stromal Cell --- Mesenchymal Stromal Cells --- Multipotent Bone Marrow Stromal Cell --- Multipotent Mesenchymal Stromal Cell --- Adipose Derived Mesenchymal Stem Cell --- Adipose Derived Mesenchymal Stem Cells --- Adipose Derived Mesenchymal Stromal Cells --- Adipose Tissue Derived Mesenchymal Stem Cell --- Adipose Tissue Derived Mesenchymal Stem Cells --- Adipose Tissue Derived Mesenchymal Stromal Cells --- Mesenchymal Stem Cells, Adipose Derived --- Progenitor Cell, Mesenchymal --- Progenitor Cells, Mesenchymal --- Stem Cell, Mesenchymal --- Stromal Cell, Mesenchymal --- Stromal Cells, Mesenchymal --- Wharton's Jelly Cell --- Whartons Jelly Cells --- Induced pluripotent stem cells. --- Induced pluripotent stem cells --- Induced Pluripotent Stem Cells --- Mesenchymal Stem Cells --- Mesenchymal Stem Cell Transplantation --- Induced Pluripotent Stem Cells. --- Mesenchymal Stem Cell Transplantation.

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Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potential of pluripotent stem cells in basic and translational research and in cell-based therapies. This comprehensive and authoritative atlas summarizes more than a decade of experience accumulated by a leading research team in this field. Hands-on step-by-step guidance for the derivation and culturing of human pluripotent stem cells in defined conditions (animal product-free, serum-free, feeder-free) and in non-adhesion suspension culture are provided, as well as methods for examining pluripotency (embryoid body and teratoma formation) and karyotype stability.   The Atlas of Human Pluripotent Stem Cells - Derivation and Culturing will serve as a reference and guide to established researchers and those wishing to enter the promising field of pluripotent stem cell research.

Stem cells -- Atlases. --- Stem cells. --- Stem cells --- Multipotent stem cells --- Publication Formats --- Stem Cells --- Adult Stem Cells --- Culture Techniques --- Cells --- Publication Characteristics --- Clinical Laboratory Techniques --- Investigative Techniques --- Anatomy --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cell Culture Techniques --- Atlases --- Induced Pluripotent Stem Cells --- Pluripotent Stem Cells --- Biology --- Health & Biological Sciences --- Microscopy --- Cytology --- Human cell culture. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Life sciences. --- Biotechnology. --- Cell biology. --- Microscopy. --- Life Sciences. --- Biological Microscopy. --- Stem Cells. --- Cell Biology. --- Cell culture --- Cytology. --- Chemical engineering --- Genetic engineering --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Cell biology --- Cellular biology --- Cytologists

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Tissue engineering and regenerative medicine is a rapidly evolving research field which effectively combines stem cells and biologic scaffolds in order to replace damaged tissues. Biologic scaffolds can be produced through the removal of resident cellular populations using several tissue engineering approaches, such as the decellularization method. Indeed, the decellularization method aims to develop a cell-free biologic scaffold while keeping the extracellular matrix (ECM) intact. Furthermore, biologic scaffolds have been investigated for their in vitro potential for whole organ development. Currently, clinical products composed of decellularized matrices, such as pericardium, urinary bladder, small intestine, heart valves, nerve conduits, trachea, and vessels, are being evaluated for use in human clinical trials. Tissue engineering strategies require the interaction of biologic scaffolds with cellular populations. Among them, stem cells are characterized by unlimited cell division, self-renewal, and differentiation potential, distinguishing themselves as a frontline source for the repopulation of decellularized matrices and scaffolds. Under this scheme, stem cells can be isolated from patients, expanded under good manufacturing practices (GMPs), used for the repopulation of biologic scaffolds and, finally, returned to the patient. The interaction between scaffolds and stem cells is thought to be crucial for their infiltration, adhesion, and differentiation into specific cell types. In addition, biomedical devices such as bioreactors contribute to the uniform repopulation of scaffolds. Until now, remarkable efforts have been made by the scientific society in order to establish the proper repopulation conditions of decellularized matrices and scaffolds. However, parameters such as stem cell number, in vitro cultivation conditions, and specific growth media composition need further evaluation. The ultimate goal is the development of “artificial” tissues similar to native ones, which is achieved by properly combining stem cells and biologic scaffolds and thus bringing them one step closer to personalized medicine. The original research articles and comprehensive reviews in this Special Issue deal with the use of stem cells and biologic scaffolds that utilize state-of-the-art tissue engineering and regenerative medicine approaches.

nerve conduit --- tissue engineering --- regenerative medicine --- mixed lymphocyte reaction --- histological images --- future scaffold engineering --- multiparameter --- 3DPVS --- MSCs --- Wnt signaling --- Mesenchymal Stromal Cells --- factorial design --- novel scaffold --- Wharton’s Jelly tissue --- stem cells --- umbilical arteries --- SDS --- platelet rich plasma --- TGF? signaling --- traditional scaffold --- pluripotency and commitment --- tissue engineered construct --- HLA-G --- CHAPS --- platelets --- proteomic analysis --- vibrating nature of universe. --- VS55 --- cell culture --- FGF signaling --- evolution of scaffold --- dynamicity and dimensionality --- fibrin gel --- scaffold classification --- decellularization --- vitrification --- seven-folder logics --- IIEF-5 questionnaire --- TGF-?1 --- erectile dysfunction --- human induced pluripotent stem cells --- iPSCs --- scaffolds --- Barret’s esophagus --- nerve regeneration --- long term storage --- laws of system evolution --- scaffold categorization --- platelet lysate --- 3D scaffold --- esophagus --- language of relativity --- cord blood units