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Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as

Enzymes --- Enzyme activity --- antibodies --- Immunoenzyme techniques --- Enzyme-linked immunosorbent assay --- Molecular biology --- Technique. --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis

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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. .

Enzyme-linked immunosorbent assay. --- Biomedical engineering. --- Immunology. --- RNA-ligand interactions. --- Biotechnology. --- Bioinformatics. --- Biomedical Engineering. --- Biological and Medical Physics, Biophysics. --- Protein-Ligand Interactions. --- Computational Biology/Bioinformatics. --- ELISA --- Enzyme immunoassay --- Enzyme labeled antibody technique --- Enzyme labeled immunoassay --- Enzyme linked immunoassay --- Enzyme linked immunospecific assay --- Immunoassay, Enzyme --- Immunoenzymometric assay --- Immunoenzyme technique --- Solid-phase analysis --- Biomedical Engineering and Bioengineering. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Chemical engineering --- Genetic engineering --- Immunobiology --- Life sciences --- Serology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Data processing --- Biophysics. --- Biological physics. --- Proteins . --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biological physics --- Medical sciences --- Physics