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Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Cell lines. --- Cell culture. --- Cultures (Biology) --- Cytology --- Cell culture --- Technique --- Medicine. --- Cytology. --- Genetic engineering. --- Biochemistry. --- Biomedicine general. --- Cell Biology. --- Genetic Engineering. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Composition --- Cell biology. --- Biomedicine, general. --- Health Workforce --- Medicine --- Biotechnology. --- Biomedical Research. --- Chemical Bioengineering. --- Research. --- Chemical engineering --- Genetic engineering --- Biological research --- Biomedical research

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Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.

Immunoglobulins -- Biotechnology. --- Immunoglobulins. --- Immunology. --- Immunoglobulins --- Serum Globulins --- Immunoproteins --- Blood Proteins --- Globulins --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Antibodies --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Biotechnology --- Immune globulins --- Immune serum globulin --- Medicine. --- Biomedicine. --- Biomedicine general. --- Blood proteins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Health Workforce --- Biomedicine, general. --- Medicine --- Biomedical Research. --- Research. --- Biological research --- Biomedical research

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Integrating advances in molecular biology into bioprocesses presents a continuous challenge to scientists and bioengineers. This series is conceived to help meet this challenge. It examines and assesses the feasibility of new approaches for the modification of cellular function such as gene expression, protein processing, secretion, glycosylation, immortalisation, proliferation, and apoptosis as well as the systematic study of the metabolic genotype-phenotype relationship. The series provides detailed coverage of the methodology for improving cellular properties of cells used in the production of biopharmaceuticals, gene and cell therapies and tissue engineering. It also seeks to explain the cellular mechanisms underlying in vitro physiological activity and productivity. This volume, which is based on presentations at the `European Workshop on Animal Cell Engineering' held in Costa Brava, Spain, contains a collection of chapters relating to cellular function and modification by leading authorities in several different areas of basic research and the biopharmaceutical industry.

Animal cell biotechnology. --- Genetic engineering. --- Genetic regulation. --- Cell proliferation. --- Zoology. --- Biotechnology. --- Cytology. --- Morphology (Animals). --- Biomedical engineering. --- Pharmacy. --- Cell Biology. --- Animal Anatomy / Morphology / Histology. --- Biomedical Engineering and Bioengineering. --- Cell biology. --- Animal anatomy. --- Chemistry --- Medicine --- Drugs --- Materia medica --- Pharmacology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Animal anatomy --- Animals --- Biology --- Physiology --- Cell biology --- Cellular biology --- Cells --- Chemical engineering --- Genetic engineering --- Natural history --- Anatomy --- Animal cells in biotechnology --- Animal biotechnology --- Cell renewal --- Cellular proliferation --- Cell cycle --- Cell division --- Cell populations --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Gene expression --- Gene expression regulation --- Gene regulation --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- Growth --- Regulation --- Anatomy, Comparative. --- Animal Anatomy.

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A comprehensive guide to the revolutionary area of systems biology and its application in cell culture engineering, this volume presents an overall picture of the current topics central to structural and functional genomics, proteomics, metabolomics and bioinformatics, including such hot topics as RNAi, metabolic engineering and unfolded protein response. It includes reviews of the cellular response of environmental modulation such as low temperature and osmolarity, critical assessments of the applications of metabolomics and fluxomics approaches, examination of the utility of modulation of key genes and a presentation of a theory of chemical organisation which provides a new view of the system's structure. The clearly written chapters by experts in the field describe methods applicable to investigating the unique facets of cell culture. The book should be of interest to all those working in cell culture development and drug discovery in pharmaceutical and biotechnology companies as well as in academic institutions. It provides an invaluable resource for students and researchers in biotechnology, cell culture, genomics and bioinformatics.

Cell physiology. --- Biological systems. --- Biosystems --- Systems, Biological --- Biology --- System theory --- Systems biology --- Cell function --- Cytology --- Physiology --- Philosophy

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With the discovery of stem cells capable of multiplying indefinitely in culture and differentiating into many other cell types in appropriate conditions, new hopes were born in repair and replacement of damaged cells and tissues. The features of stem cells may provide treatment for some incurable diseases with some therapies are already in clinics, particularly those from adult stem cells. Some treatments will require large number of cells and may also require multiple doses, generating a growing demand for generating and processing large numbers of cells to meet the need of clinical applications. With this in mind, our aim is to provide a book on the subject of stem cells and cell therapy for researchers and students of cell biotechnology, bioengineering and bioproduction. This book is exceptional as it teaches researchers stem cells and cell therapy in that it covers the concepts and backgrounds necessary so that readers get a good understanding of the production of stem cells. The book covers three topics: The basics of stem cells and cell therapy, the use of stem cells for the treatment of human diseases, and stem cell processing. It includes chapters on neural and vascular stem vascular stem cell therapy, expansion engineering of embryonic stem cells, stem cell based production of blood cells and separation technologies for stem cells and cell therapy products. It is an informed and informative presentation of what modern research, science and engineering have learned about stem cells and their production and therapies. Addressing both the medical and production issues, this book is an invaluable contribution to having an academic and industrial understanding with respect to R&D and manufacturing of clinical grade stem cells.

Cellular therapy. --- Stem cells. --- Brain -- Diseases -- Treatment. --- Neural stem cells. --- Medicine. --- Cell biology. --- Cell culture. --- Biomedicine. --- Biomedicine general. --- Cell Biology. --- Stem Cells. --- Cell Culture. --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Cell therapy --- Therapy, Cellular --- Organotherapy --- Therapeutics, Physiological --- Transplantation of organs, tissues, etc. --- Cell transplantation --- Therapeutic use --- Cytology. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Cultures (Biology) --- Cytology --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Technique --- Health Workforce --- Biomedicine, general. --- Medicine --- Biomedical Research. --- Stem Cell Biology. --- Cytological Techniques. --- Research. --- Technique. --- Biological research --- Biomedical research