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dissertation (6)

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French (10)


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Dissertation
Etude de l'activité d'un promoteur de glutenine de haut poids moléculaire en expression transitoire dans des protoplastes de feuilles de Nicotiana plumbaginifolia
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Year: 1988 Publisher: [S.l. : Chez l'auteur],

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Book
Protoplastes, fusion somatique et métabolisme secondaire.
Authors: ---
Publisher: [S.l.] : Rennes : Rennes : AUPELF-UREF ; CNED (Centre National d'Enseignement à Distance) ; Institut de Rennes.

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Dissertation
Contribution à l'étude du processus d'activation du virus de la mosaïque à tirets dans les protoplastes de Musa spp.
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Year: 2002

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Dissertation
Production d'haploïdes et hybridation somatique chez le piment, Capsicum annuum L.
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Year: 1997 Publisher: Gent : Universiteit Gent. Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen,

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Dissertation
Etude de la régénération de plantules de pomme de terre à partir de protoplastes : effets du génotype et des milieux de culture.
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Year: 1993

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Dissertation
Etude des techniques d'obtention de protoplastes de poirier (Pyrus communis var. Durondeau).
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Year: 1988

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Dissertation
Culture de protoplastes et contaminants procaryotiques chez un clone de Prunus cerasus L. 'Montmorency'.
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Year: 1996

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A protocol for the isolation of protoplasts from leaf mesophyll, leaf and root calluses of Prunus cerasus L. "Montmorency" was developed. The experimental factors investigated were : the age and the physiological status of the source materials, the pre-treatment of source tissues, the enzymatic treatment (source, concentration and specific activity of enzymes), the incubation time, and the isolation protocol. Bacterial contaminations were revealed after protoplast isolation. These bacteria were formed from endophytic contaminants present as wall-free prokaryote cells, as observed in the micropropagated plants by electron microscopy, or after grinding leaf tissues in solutions with high osmotic pressure. After synthesis of a cell wall and subsequent development on nutritive media, four contaminating bacterial strains were identified as Pseudomonas syringae and Agrobacterium rhizogenes species. The amplification by PCR of the gene coding for a receptor protein of siderophores in fluorescent species of Pseudomonas, confirmed the presence of those wall free prokaryote contaminants in mesophyll tissues of apparently healthy micropropagated plants. In presence of antibiotics, leaf callus protoplasts remained alive for 20 to 25 days on MHABZ medium. During this period, the synthesis of a new cell wall was observed in 43 % of leaf callus protoplasts after 10 to 20 days of incubation, and first divisions were observed in 17.6 % after 15 to 20 days of incubation.


Book
Colloque international sur la culture " in vitro " des essences forestières, Fontainebleau, France, 31 août-4 septembre 1981 : exposés
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Year: 1981 Publisher: Nangis (France) : AFOCEL (Association FOrêt-CELlulose),

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Book
Cultures de cellules, tissus et organes végétaux : fondements théoriques et utilisations pratiques
Authors: --- --- ---
ISBN: 2880741521 9782880741525 Year: 1988 Publisher: Lausanne : Presses Polytechniques Romandes,

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