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Western immunoblotting --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Immunoblotting, Western --- Protein blotting --- Western blot analysis --- Western blotting --- Immunoblotting --- Proteins --- Analysis

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  A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions.  The method that is most widely used is the Western blot analysis.  While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations.  In particular, the method is semi-quantitative with limited dynamic range.  Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment.  New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins.  One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM).  Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites.  The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages.  Most importantly, it is fundamentally quantitative with a wide dynamic range.  The output of the analysis is a numerical result that can range over several orders of magnitude.  Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures.  Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment.     This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins.

Spectrometric and optical chemical analysis --- Genetics --- General biochemistry --- Human biochemistry --- Pathological biochemistry --- Human medicine --- massaspectrometrie --- western blotting --- medische biochemie --- proteomics --- biochemie --- biomedische wetenschappen --- moleculaire biologie --- proteïnen

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Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Me

Nucleic acid probes --- Polymerase chain reaction --- Virus diseases --- -Molecular diagnosis --- 57.083 --- 616-093 --- HIV human immunodeficiency virus --- NASBA --- PCR polymerase chain reaction --- biochemie --- biologie --- blotting --- chemiluminescentie --- diagnostiek --- geneeskunde --- hybridisatie --- kwaliteitscontrole --- laboratoriumbiologie --- ligase kettingreactie --- nucleïnezuren --- virusinfecties --- virussen --- Viral diseases --- Viral infections --- Virus infections --- Communicable diseases --- Medical virology --- Pathogenic viruses --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Oligonucleotide probes --- Probes, Nucleic acid --- Molecular probes --- Molecular diagnosis --- Polymerase chain reaction. --- Nucleic acid probes. --- Molecular diagnosis. --- Virus diseases - - Molecular diagnosis

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This text gives a broad, but concise, coverage of gene cloning and manipulation, suitable for undergraduates and beginning graduate students. Assuming only general biochemical knowledge, it stresses the concepts underlying particular types of cloning vector, and uses examples to illustrate them, rather than simply presenting a mass of detailed lists and vector maps. The book starts by describing the principles behind cloning DNA in E. coli, the enzymes used, the range of cloning vectors available, and how to screen libraries to find particular clones. The author shows how PCR can be used as an alternative, or complementary, approach. He then goes on to describe how sequences can be exploited, after cloning and identification, by site-directed mutagenesis and over-expression. The book finishes with a detailed presentation of the genetic manipulation of other organisms, including other bacteria, yeast, plants, insects, and mammals.

genetische engineering (manipulatie) --- kloneren (klonen) --- genetica (genen) --- génie génétique (ingénierie génétique) --- clonage --- génétique (gènes) --- PCR polymerase chain reaction --- bacteriofagen --- blotting --- clonen --- gastheerorganismen --- gelelektroforese --- laboratoriumbiologie --- plasmiden --- rDNA recombinant DNA --- restrictie enzymen --- vectoren biologie --- biologische technieken, experimentele methoden en uitrusting in het algemeen --- Molecular cloning --- 57.08 --- 577.21 --- biochemie --- biologie --- genetica --- genetische manipulatie --- modificatie --- moleculaire biologie --- mutagenen --- #ABIB:aimm --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.

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  A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions.  The method that is most widely used is the Western blot analysis.  While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations.  In particular, the method is semi-quantitative with limited dynamic range.  Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment.  New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins.  One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM).  Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites.  The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages.  Most importantly, it is fundamentally quantitative with a wide dynamic range.  The output of the analysis is a numerical result that can range over several orders of magnitude.  Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures.  Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment.     This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins.

Biomedicine. --- Data mining. --- Proteins -- Analysis. --- Proteomics. --- Proteomics --- Proteins --- Amino Acids, Peptides, and Proteins --- Biochemistry --- Immunoblotting --- Immunoassay --- Biological Science Disciplines --- Chemicals and Drugs --- Immunologic Tests --- Natural Science Disciplines --- Disciplines and Occupations --- Laboratory Techniques and Procedures --- Diagnosis --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Blotting, Western --- Analysis --- Analysis. --- Methodology. --- Medicine. --- Mass spectrometry. --- Biochemistry. --- Biomedicine general. --- Mass Spectrometry. --- Biochemistry, general. --- Molecular biology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Mass spectra --- Mass spectrograph --- Mass spectroscopy --- Mass spectrum analysis --- Mass (Physics) --- Nuclear spectroscopy --- Spectrum analysis --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Composition --- Health Workforce --- Biomedicine, general.